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Development of a Taqman Real-Time PCR Assay for Detection of Bordetella bronchiseptica

Development of a Taqman Real-Time PCR Assay for Detection of Bordetella bronchiseptica

Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2

E-mail | Tomas.Jinnerot@sva.se

1Department of Microbiology, National Veterinary Institute, SE-751 89 Uppsala, Sweden; 2Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O Box 7054, SE-750 07 Uppsala, Sweden.

Figure 1

Amplification efficiency of the bfrZ real-time PCR. A standard curve was generated by 10-fold serial dilutions of B. bronchiseptica CCUG 219 DNA. PCR in six replicates revealed a good linear relationship (E=95%) and a wide dynamic range.

Veterinary Sciences: Research and Reviews

June

Vol.10, Iss.1, Pages 1-39

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