Development of a Taqman Real-Time PCR Assay for Detection of Bordetella bronchiseptica
Development of a Taqman Real-Time PCR Assay for Detection of Bordetella bronchiseptica
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
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Amplification efficiency of the bfrZ real-time PCR. A standard curve was generated by 10-fold serial dilutions of B. bronchiseptica CCUG 219 DNA. PCR in six replicates revealed a good linear relationship (E=95%) and a wide dynamic range.