Development of a Taqman Real-Time PCR Assay for Detection of Bordetella bronchiseptica
Tomas Jinnerot1*, Karin Malm1, Erik Eriksson1 and Jonas Johansson Wensman2
E-mail | Tomas.Jinnerot@sva.se
1Department of Microbiology, National Veterinary Institute, SE-751 89 Uppsala, Sweden; 2Department of Clinical Sciences, Swedish University of Agricultural Sciences, P.O Box 7054, SE-750 07 Uppsala, Sweden.
Figure 1
Amplification efficiency of the bfrZ real-time PCR. A standard curve was generated by 10-fold serial dilutions of B. bronchiseptica CCUG 219 DNA. PCR in six replicates revealed a good linear relationship (E=95%) and a wide dynamic range.