Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig
Effect of Chronic Adrenocorticotropine Hormone Treatment on LPS-induced Slight Inflammation via Inhibiting Nuclear Translocation of Glucocorticoid-glucocorticoid Receptor Complex in Lung of Pig
Zhiyuan Sun1, 3, Xuhui Zhang2,*, Xian Li3, Yanping Huang3, Shiyan Sui3, Weifeng Liu3, Guochao Ni3, Xiaozhou Xu1, Jianbo Yang1, Xue Lian1 and Hui Jia1
Effects of LPS/ACTH on serum TNF-α concentration. Pigs were administered LPS injection with or without continuous ACTH pretreatment. TNF-α protein level in serum was determine by ELISA. TNF-α, Tumor necrosis factor; LPS, lipopolysaccharide; ACTH, adrenocorticotropine. Mean values with different letters (a, b, c, or d) are significantly different (P <0.05) from each other. n = 5-6.
Expression of TLR2 mRNA (a) TLR4 mRNA (b) in porcine lung tissue. Pigs were administered LPS injection with or without continuous ACTH pretreatment. TNF-α protein level in serum was determine by ELISA. TLR, toll-like receptor 2; LPS, lipopolysaccharide; ACTH, adrenocorticotropine hormone. b-actin was chosen as a reference for normalization in the qPCR and Western blot analyses. Mean values with different letters (a, b, c, or d) are significantly different (P <0.05) from each other. n = 5-6.
GR mRNA (a) total GR protein (b) cytoplasmic (c) and nuclear (d) GR protein content in porcine lung tissue.
