Submit or Track your Manuscript LOG-IN

Effectiveness of Butylated Hydroxytoluene in Maintaining the Quality of Gaga Chicken Sperm in Liquid Storage for 72 Hours

Effectiveness of Butylated Hydroxytoluene in Maintaining the Quality of Gaga Chicken Sperm in Liquid Storage for 72 Hours

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

1Doctoral Program in Animal Science, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 2Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; 3Livestock Reproduction Laboratory, Faculty of Animal Science, Universitas Hasanuddin Jl. Perintis Kemerdekaan, Makassar 90245, South Sulawesi, Indonesia; 4Politeknik Pembangunan Pertanian Malang, Jl. DR. Cipto No.144a, Malang 65215, East Java, Indonesia.

 
*Correspondence | Sri Wahjuningsih, Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; Email: yuning@ub.ac.id

ABSTRACT

Artificial insemination is beneficial for the poultry industry and breeding programs due to the ability to efficiently use superior male chickens for mating with multiple females. This study aimed to examine the effect of adding BHT (butylated hydroxytoluene) into the diluent and storage time on the quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment without BHT. Liquid semen was stored for 72 hours at 5°C, with spermatozoa quality monitored every 24 hours in 10 replicated experiments. The results showed that sperm motility, viability, and plasma membrane integrity were significantly higher (P<0.01) when 2-3 mM BHT was added, while acrosome integrity was elevated after the addition of 1 and 3 mM BHT. Furthermore, the addition of BHT did not significantly change semen pH. DNA damage was lower (P<0.05) when 3 mM BHT was added and mitochondrial activity was enhanced under the same condition. The concentration of malondialdehyde (MDA) was lower (P=0.05) with the addition of 3 mM BHT at 24 hours of storage. In conclusion, the addition of 3 mM BHT showed promising potential to maintain chicken sperm quality after liquid storage.
 
Keywords | Butylated hydroxytoluene, Gaga chicken, Diluent, Liquid storage, Semen, Sperm

To share on other social networks, click on any share button. What are these?

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

Featuring

Click here for more

Subscribe Today

Receive free updates on new articles, opportunities and benefits


Subscribe Unsubscribe