Pankaj Kumar Jha1,2*, M. Golam Shahi Alam1 and Farida Yeasmin Bari1
Pregnancy and Lambing Rate Following Laparoscopic Artificial Insemination with Two Different Types of Diluent and Frozen-thawed Sperm Dose in Ewes
...h two different types of diluent (commercial Triladyl® and home-made tris based) and number of frozen-thawed ram sperm (100 × 106 and 50 × 106 sperm dose) on pregnancy and lambing rate following laparoscopic artificial insemination (LAP-AI) in indigenous ewes in Bangladesh were studied. A total of 78 non- pregnant ewes were synchronized for oestrus with two doses of 100 μg PGF2α (Cloprostenol) 9 days apart. The ewes were randomly assig...
Ibrar Muhammad Khan1, Dezhi Xu1, Zubing Cao1, Hongyu Liu1, Adnan Khan2, Sajid Ur Rahman3, Jam Zaheer Ahmed4, Muhammad Akmal Raheem5 and Yunhai Zhang1*
...E) were added into semen diluent and assessed the freeze-thawed semen quality parameters. The ejaculates were collected from two crossbred groups (Sahiwal × Holstein-Friesian), and (Achai × Jersey) and, split into seven aliquots while extended with three different concentration (5, 7.5 and10 mmol) of L-cysteine diluents and followed by other three concentrations (1.2, 2.4 and 4.8 mmol) of vitamin E ...
Uzma Hameed1*, Aneela Liaqat1, Muhammad Asjad Khan2, Ikram-Ul-Haq1 and Maham Aslam1
... U/ml) was observed when diluent with initial pH of 7 was used. Among different carbon sources (maltose, glucose, starch, galactose, arabinose and sucrose) best results were obtained with soluble starch. Alpha-amylase production is sensitive to the supply of nitrogen sources. In this context, both inorganic and organic nitrogen sources, were evaluated. Optimal enzyme production was obtained with diluent supplemented with NaN...
Tinda Afriani*, Jaswandi, Adisti Rastosari, Muhammad Cadilac Al Razzak, Dwiki Wahyudi
...tive in egg yolk citrate diluent on the quality of Pesisir cattle semen stored at 5oC. The study was conducted on freshly collected Pesisir cattle semen diluted in egg yolk citrate with or without tomato juice viz., P0 (100% egg yolk citrate), P1 (90% egg yolk citrate + 10% tomato juice) and P2 (80% egg yolk citrate + 20% tomato juice). After diluting the semen samples were stored at 5oC for 4 hours. The observed variables included spermatozoa motility, sperma...
Sigit Bintara1*, Riyan Nugroho Aji1, Panjono2, Ali Agus3
...ed some egg yolk citrate diluent of 2.9% (v/v), Sodium citrate dihydrate buffer, 100 mL of distilled water, and an egg yolk mixture to a proportion of 20% egg yolk 8% (v/v) glycerol (Merck, Germany), penicillin (1,000 IU/mL), and streptomycin (1,000 mg/mL). Sperm was diluted to 50 million/mL and treated with lycopene extract levels of 0% (control), 1%, 2%, 3%, and 4% in a diluent, equilibrated at the temperature of 5°C f...
Chairdin Dwi Nugraha1, Rafika Febriani Putri1, Ari Ardiantoro2, Wike Andre Septian1, Ahmad Furqon3, Kuswati Kuswati1, Nashi Widodo4, Suyadi Suyadi1*
...lementation in the basic diluent of tris-aminomethane on the quality of spermatozoa. Bulls aged 3–4 years used in this research. This research used an experimental method to examine the effect of genistein levels in the diluent on the characteristics of spermatozoa during pre-freezing at 4–5 0C and post-thawing (PT). The data obtained in the form of quantitative data were analyzed descriptively and analysis of va...
Lukmanhy1*, Enny Yuliani1, I Wayan Lanus Sumadiasa1, Lalu Ahmad Zaenuri1, Mardiansyah2
... to egg yolk tris-buffer diluent on the semen quality of Kacang goats at 5°C storage. Semen samples were collected twice a week from a 2-year-old Kacang goat using an artificial vagina. The study design consisted of three treatments; T0, T1, T2 and T3, consisting of 0%, 10%, 15%, and 20 % of sugarcane juice, respectively. The measured parameters included progressive motility, viability, and abnormalities of spermatozoa. The data obtained were analyzed usin...
Rizkie Elvania1, Tintin Rostini1, Sakiman2, Ani Susilawati2, Emilda Adriani2, Abd Malik1*
...o the skim milk-egg yolk diluent at concentrations of 1%, 2%, 3%, and 4%, while the control group received no lycopene substitution. The freshly collected semen underwent macroscopic and microscopic evaluations. Subsequently, the semen was assessed pre-freezing and post-thaw with parameters such as viability, motility, abnormality, and plasma membrane integrity. The findings revealed that the inclusion of 1% and 2% lycopene in the dilu...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
...hydroxytoluene) into the diluent and storage time on the quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, 2, and 3 mM, respectively as well as a control treatment wit...
Nurcholis1*, Syetiel M. Salamony1, Abdullah Baharun2
...ity. Antioxidants in the diluent can maintain the motility of bull sperm during the cooling process until after thawing. This research examines the effect of Myrmecodia extract (MYE) supplementation, which has high antioxidants, on semen diluent during the cooling to cryopreservation process. Specifically, five PO bulls aged four years were collected using an artificial vagina (AV) twice weekly at the Semangga District...
Khaeruddin1,2, Gatot Ciptadi1, Muhammad Yusuf3, Hermawansyah2, Sahiruddin3 and Sri Wahjuningsih1*
...emination techniques and diluents are important factors in determining the quality of chicken spermatozoa. The aim of this study was to determine the effect of the use of three types of crystallized liquids and two types of monosaccharides on the quality of Gaga chicken spermatozoa. This study used a completely randomized design with a factorial pattern with the first factor being the type of crystalloid liquid (Ringer’s lactate, Ringer’s acetate, ...
Aulia Puspita Anugra Yekti, Ani Atul Arif, Putri Utami, Habib Asshidiq Syah, Anggita Dian Pramudhita, Rr. Ani Rizqianti, Trinil Susilawati*
...on various gradients and diluents in Belgian Blue bull. The research material used was a semen sample of Belgian Blue cross bull with progressive motility of fresh semen of≥ 70%. The research used a Completely Randomized Design (CRD) with four treatments and ten replications. Data were analyzed using the Tukey test. The treatment in the study was T0: sexing PDGC 10 fractions using Andromed® diluent. T1: sexing PDGC 10...
Sigit Bintara1*, Diah Tri Widayati1 and Pradita Iustitia Sitaresmi2
... C. xanthorriza in semen diluent significantly enhanced post-thaw sperm quality in thin-tailed ram semen by reducing free radical-mediated oxidative stress during pre-freezing or after thawing....
Wilmienthe Marlene Mesang Nalley1*, Aloysius Marawali1, Kirenius Uly1, Petrus Kune1, Franky Ms Telupere1, Ni Made Paramita Setyani1, Alvrado Bire Lawa1, Yustiany Yuliana Bette1, Thomas Mata Hine1, Abdullah Baharun2
...uctose) to Tris-egg yolk diluents , providing additional energy substrates that may extend sperm viability and motility. Good-quality sperm samples, with motility levels of ≥70% and abnormalities <20%, were diluted in a TEy control solution, with additional treatments including sucrose (TS), lactose (TL), fructose (TF), and combinations of the carbohydrates (TSF, TLF). The diluted samples were stored at 18-20°C and evaluated at 12-hour interval...
Andi Nirmala1, Abdul Latief Toleng2, Muhammad Yusuf2*, Herdis Herdis3, Athhar Manabi Diansyah2,3, Muhammad Fajar Amrullah2, Rahmat Rahmat2,5, Rajamuddinn Rajamuddin1, Andi Muhammad Alfian1, Hasrin Hasrin4
...extract to Tris-Egg yolk diluent on the quality of Bali bull spermatozoa. The study was designed by comparing four treatments: T0 (non-Kasumba Turate), T1 (0.5% Kasumba Turate extract), T2 (1% Kasumba Turate extract), and T3 (1.5% Kasumba Turate extract). The semen was diluted with Tris-Egg yolk diluent containing Kasumba Turate extract, along with penicillin and streptomycin antibiotics. After equilibration at 3-5°C for...
