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Effectiveness of Butylated Hydroxytoluene in Maintaining the Quality of Gaga Chicken Sperm in Liquid Storage for 72 Hours

Effectiveness of Butylated Hydroxytoluene in Maintaining the Quality of Gaga Chicken Sperm in Liquid Storage for 72 Hours

Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*

1Doctoral Program in Animal Science, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 2Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; 3Livestock Reproduction Laboratory, Faculty of Animal Science, Universitas Hasanuddin Jl. Perintis Kemerdekaan, Makassar 90245, South Sulawesi, Indonesia; 4Politeknik Pembangunan Pertanian Malang, Jl. DR. Cipto No.144a, Malang 65215, East Java, Indonesia.

 
*Correspondence | Sri Wahjuningsih, Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; Email: yuning@ub.ac.id

Figure 1:

Evaluation of chicken sperm viability using eosin-nigrosin staining. a: dead sperm, b: alive sperm) (left), and evaluation of DNA damage in chicken sperm using toluidine blue staining (c: damaged DNA, d: intact DNA) (right).

Figure 2:

Evaluation of plasma membrane integrity of chicken sperm using HOST (left: intact plasma membrane, right: damaged plasma membrane).

Figure 3:

Evaluation of acrosome integrity of chicken sperm using coomassie brilliant blue staining (left: intact acrosome, right: damaged acrosome).

Figure 4:

DNA damage of Gaga chicken sperm with the addition of BHT 3 mM in diluent during cold storage (%) (n=10).

Figure 5:

Mitochondrial activity of Gaga chicken sperm with the addition of BHT 3 mM in diluent during cold storage (%) (n=10).

Figure 6:

Evaluation of mitochondrial activity of chicken sperm using DAB assay.

Figure 7:

MDA levels of Gaga chicken semn with the addition of BHT 3 mM in diluent during cold storage (n=10).

Figure 8:

pH of Gaga chicken semen with the addition of in diluent during cold storage (n=10).

Advances in Animal and Veterinary Sciences

November

Vol. 12, Iss. 11, pp. 2062-2300

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