Effectiveness of Butylated Hydroxytoluene in Maintaining the Quality of Gaga Chicken Sperm in Liquid Storage for 72 Hours
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
1Doctoral Program in Animal Science, Faculty of Animal Science, University of Brawijaya, Jl. Veteran, Malang 65145, East Java, Indonesia; 2Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; 3Livestock Reproduction Laboratory, Faculty of Animal Science, Universitas Hasanuddin Jl. Perintis Kemerdekaan, Makassar 90245, South Sulawesi, Indonesia; 4Politeknik Pembangunan Pertanian Malang, Jl. DR. Cipto No.144a, Malang 65215, East Java, Indonesia.
*Correspondence | Sri Wahjuningsih, Department of Animal Science, Faculty of Animal Science, University of Brawijaya. Jl. Veteran, Malang 65145, East Java, Indonesia; Email: yuning@ub.ac.id
Figure 1:
Evaluation of chicken sperm viability using eosin-nigrosin staining. a: dead sperm, b: alive sperm) (left), and evaluation of DNA damage in chicken sperm using toluidine blue staining (c: damaged DNA, d: intact DNA) (right).
Figure 2:
Evaluation of plasma membrane integrity of chicken sperm using HOST (left: intact plasma membrane, right: damaged plasma membrane).
Figure 3:
Evaluation of acrosome integrity of chicken sperm using coomassie brilliant blue staining (left: intact acrosome, right: damaged acrosome).
Figure 4:
DNA damage of Gaga chicken sperm with the addition of BHT 3 mM in diluent during cold storage (%) (n=10).
Figure 5:
Mitochondrial activity of Gaga chicken sperm with the addition of BHT 3 mM in diluent during cold storage (%) (n=10).
Figure 6:
Evaluation of mitochondrial activity of chicken sperm using DAB assay.
Figure 7:
MDA levels of Gaga chicken semn with the addition of BHT 3 mM in diluent during cold storage (n=10).
Figure 8:
pH of Gaga chicken semen with the addition of in diluent during cold storage (n=10).