Establishment of Multiplex PCR for Detection of Calf Diarrhea Associated Virus and Analysis of its Clinical Infection Status
Liyun Chang*, Yazi Li, Yumei Cai and Chenghui Li
Faculty of Life Science, Tangshan Normal University, Thangshan, Hebei, 064001, China
Liyun Chang and Yazi Li contributed equally to this work.
* Corresponding author: changliyun2006@163.com
Fig. 1.
PCR amplification of (a) BVDV DNA (b) BRV DNA and (c) BCV DNA. M, DL1000 Marker Lane 2 is Negative control.
Fig. 2.
Optimization of the annealing temperature (A), PCR primer concentraction; (B), multiplex PCR primer concentration.
Fig. 3.
Results of the multiplex PCR specificity (A), and sensitivity (B) test.