Feeding a High-Concentrate Diet Reduces Milk Production is Associated with Endogenous Growth Hormone in Lactating Goats

Ping Sheng Ye1, Lin Li2, Yu Long Wu3 and Yuan Shu Zhang1*

1Key Laboratory of Animal Physiology and Biochemistry, Ministry of Agriculture, Nanjing Agricultural University, PR China

2School of Biological Science and Engineering, Xingtai University, Xingtai 054001, China

3The Food Science College, Nanjing Xiaozhuang University, PR China

ABSTRACT

The aim of this work was to evaluate the effects of endogenous GH-IGF-1 axis on lactating goats fed with high-concentrate diets. Ten lactating goats were used and randomly divided into two groups, in a 2×2 Latin square experiment design with different forage to concentrate rations of 40:60 (the control group) and 60:40 (the high-concentrate group), respectively. During the experiment, milk samples were collected to assay the content of milk compositions; plasma samples were collected to measure the content of IGF-1 and GH. The liver and mammary gland samples were collected to observe the expression of IGF-1R and GH. The results showed that compared with the control group, the content of plasma GH and IGF-1 were decreased in the high-concentrate group, and the percentage of lactose and milk yield were also decreased. Meantime, the mRNA expression of IGF-1R in mammary gland was down-regulated in the high-concentrate group. Our results indicated that feeding with high-concentrate diets for 9 weeks would decrease the contents of endogenous GH and IGF-1, and eventually lead to the decreased milk yield.

Article Information

Received 16 February 2023

Revised 09 March 2023

Accepted 27 March 2023

Available online 18 May 2023

(early access)

Published 12 April 2024

Authors’ Contribution

PSY performed the experiment, drafted the manuscript and analysed the data. LL and YLW contributed to experimental design and manuscript revision. YSZ conceived the idea, designed the experiment and finalized the manuscript. All authors read and approved the final manuscript.

Key words

High-concentrate diets, Endogenous GH, Galactopoietics effect, Milk yield, Sannen goats

DOI: https://dx.doi.org/10.17582/journal.pjz/20230216080219

* Corresponding author: [email protected]

0030-9923/2024/0003-1225 $ 9.00/0

Copyright 2024 by the authors. Licensee Zoological Society of Pakistan.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

The growth hormone (GH) plays a crucial role in ruminant mammary development, onset of lactation, and maintenance of milk secretion (Akers, 2006). GH was synthesized and secreted by the acidophilic cell of pituitary anterior lobe, and regulated by growth hormone releasing hormone and growth hormone release inhibiting hormone of the hypothalamus (Tucker, 2000). The primary function of GH is to regulate somatic growth, but many of its growth-promoting effects are indirectly mediated by stimulation of liver to produce insulin-like growth factor-1(IGF-1) (McCoard et al., 2016). In vivo, GH and IGF-1 form the GH-IGF-1 axis to produce important action to regulate cell growth, differentiation, maintenance of normal cellular function and metabolism (Berryman et al., 2008). Several studies have found that GH-IGF-1 pathway is related to lactation. It has been shown that in lactating ruminant, high levels of GH profit glucose and fat into the mammary gland, and could significantly improve milk yield. The content of plasma GH has an overall increasing trend with the increased proportion of dietary roughage. The increased proportion of roughage in total mixed ration diets can not only promote the synthesis and secretion of GH, but also contribute to the process of anabolic metabolism that improvs milk yield (Min et al., 2005).

At present, concentrate supplementation is used to compensate nutritional deficiencies in the forage supply to improve animal performance such as milk production (Tarazon-Herrera et al., 2000). However, long-time feeding with high-concentrate not only induces the decrease of milk quality and milk yield, but also causes metabolic disorders and increases the risk of various diseases (Ma et al., 2022). How to find a balance between milk yield and body health is the main focus of dairy production. The GH-IGF-1 pathway participates in lactating and is directly related to milk yield, but its change and effect on milk production under high-concrntrate feeding are unclear. In this study, we assessed the level of endogenous GH and IGF-1in lactating goats with different forage to concentrate ratio diets and analyzed the expression level of related receptor genes to reveal the effect of endogenous GH-IGF-1 axis on the decreased of milk yield caused by feeding with high-concentrate diet.

Materials and Methods

Experimental animal and experimental design

The trial was conducted in a standard animal house at Nanjing Agricultural University (Nanjing, China). Ten health multiparous dairy Sannen goats, with an average body weight of 45±7 Kg (mean ± SD), were used at mid-locating with an average milk yield of approximately 0.5 Kg.

After 14 days adaptation period for experiment environment, goats were divided into two experimental groups in a 2×2 Latin square experiment design, which were balanced for body weight, parity and milk yield. The experimental design was a completely randomized, with four replicates per treatment. One group received diets with low concentrate (40% of dry matter) as the control group, and another group received high concentrate diets (60% of dry matter) as the high-concentrate group. Ingredients and nutrient compositions of experimental diets were presented in Table I. The content of total energy and total protein were equivalent between the two experimental diets. Goats were housed in individual stalls and free access to fresh water thoughout the experimental time. Goats were fed twice daily (feeding time were from 07:00 to 08:00 h and from 19:00 to 20:00 h) and milked twice daily (the time were consistent with feeding time) for statistical daily milk yield. The health condition was checked thoughout the study period and no cases of clinical mastitis were recorded.

Sample collections

Daily milk yield of each goat was recorded by means of graduated measuring cylinders attached to individual milking units. Fifty milliliter of milk was sampled from individual goats, consisting of proportional volumes of morning (from 07:00 to 08:00 h) and evening (from 19:00 to 20:00 h). Samples were collected in 60 mL sterile plastic containers with potassium dichomate and stored at the 4℃ until milk composition analysis conducted by the commercial company of Nanjing Weigang dairy industry Co., Ltd.

We collected blood samples in the 2nd, 5th and 8th week. Samples were taken at the same hour (from 07:00 to 08:00 h) each time. At the 9th week, blood was sampled at feeding before, after feeding 15, 30, 45 and 60 min. Blood samples were obtained by puncturing of the jugular vein and stored at EDTA-containing vacuum tubes. Blood samples were centrifuged (2,300 g×15 min at 4oC), and plasma was removed and stored at -20oC until assayed.

Goats were executed at the end of experiment, the liver and mammary gland tissue samples were collected immediately, and immediately frozen in liquid nitrogen. All samples were stored at -80oC until assayed.

 

Table I. Ingredient and nutrient composition of experimental diets.

Ingredients (%)	Forge to concentrate ratio
	4:6	6:4
Leymus chinensis	40.00	26.70
Medicago sativa Hay	20.00	13.30
Corn	22.99	23.24
Bran	0	20.77
Soybean meal	15.00	13.66
Powder	0.65	1.43
DCP	0.46	0
Premixa	0.50	0.50
Salt	0.40	0.40
Total	100	100
Nutrient levels
Net energy(MJ/kg)	5.63	5.83
Digestible crude protein (%)	9.90	10.00
Neutral detergent fiber (%)	36.64	34.55
Acid detergent fiber (%)	24.74	20.35
Calcium (%)	0.80	0.90
Phosphorus (%)	0.33	0.38

 

a, Provided per kg of premix: Vitamin A 6 000U, Vitamin D 2 500U, Vitamin E 80ng, Cu 6.25mg, Fe 62.5mg, Ze 62.5mg, Mn 50mg, I 0.125mg, Co 0.125 mg, Mo 0.125 mg.

 

Analyses

IGF-1 concentration in the jugular plasma samples were determined by enzyme linked immunosorbent assay using commercial kits (Goat Insulin Like Growth Factor 1(IGF-1) ELISA Kit, Beijing Rigobio Science Development Co.,Ltd. Beijing, China). GH concentration were determined by radioimmunoassay using commercial kits (Iodine [125I] Growth Hormone Radioimmunoassay Kit, Beijing North Institute of Biotechnology, Beijing, China).

Total RNA was extracted from the liver and mammary gland tissue samples using the Trizol reagent (Takara, Dalian, China) according to the manufacturer’s instructions. The purity of the extracted RNA was verified by measuring the ratio of absorbencies at 260nm and 280nm.

 

Table II. The primer sequences of the target genes.

Gene	Genbank accession number	Primers sequence (5’-3’)	Orientation	Product size(bp)
Β-actin	JN033788	CCGTCATTAGTGCGTCAGTTC	Forward	175
		ATTCCTCACCCAGTCTTCGTC	Reverse
GH	M82912.1	TCCAGCCTCTGTTTCA	Forward	94
		CCACTGCCAAGGTCAA	Reverse
IGF-1R	JN200823.1	GCTCA ACCCA GGGAA CTACA C	Forward	161
		CCACT ATCAA CAGAA CCGCA AT	Reverse

 

The RNA was stored at -80oC until use. Total RNA was transcribed into cDNA by using standard techniques of the Superscript first strand synthesis system (Shengxin Bio, Nanjing, China). The relative expression quantification of IGF-1R and GH mRNA in liver and mammary gland were observed by the Real-time PCR method. The genes sequence were obtained from Genbank and designed with the software of Primer 5 according to the principle of primer design (the primer sequences of the target genes were reported in Table II). All primers were synthesized by Beijing Dingguo Changsheng Biotechnology Co., Ltd (Beijing, China). The SYBR Green Supermix (Bio-Rad, 10023434, USA) was used to quantify the relative abundance of target mRNA. The detection was performed on a Real-Time PCR Detection System (Bio-RAD Q5 Applied Biosystems Inc., Foster City, USA). β-actin was used as a reference gene. The theshold cycle (CT) for the test samples was subtracted from the CT for the control sample to obtain the change(delta) in CT (△CT). In order to calculate differences in the expression level of the target gene, the △△CT method for relative quantification was used according to the manufacturer’s manual.

Statistical analyses

All data were analyzed statistically by using SPSS 16.0 with independent-samples T-test. Results were expressed as the average ± standard error (mean±SE). The significance level was declared at P<0.05.

Results

Milk yield and milk compositions

In the experiment, we analyzed the change of weekly milk yield between the control group and the high-concentrate group. As shown in Figure 1, the average milk yield of goats with high-concentrate diet was lower than the control group during the 9 weeks trial time. However, there was no significant differences between the two groups.

We also collected milk samples in the 2nd and 5th week and sent to the commercial company of Nanjing Weigang dairy industry Co., Ltd to analyze the milk compositions. According to the results (Fig. 2), compared to the control group, the percentage of milk lactose in the high-concentrate group were lower.

 

 

 

Plasma IGF-1 and GH.

As shown in Figure 3A, the content of plasma IGF-1 in the high-concentrate group were significantly lower than the control group in samples collected from week 2 (P<0.05) and week 5 (P<0.01).

As shown in Figure 3B, the plasma GH levels in the 9th week samples showed decrease after feeding in both groups. And the contents of plasma GH in the high-concentrate group were lower than the control group, with no significant differences.

 

 

mRNA expression of IGF-1R and GH in liver and mammary gland tissues

The results (Fig. 4A) showed that the mRNA expression of IGF-1R and GH in the liver of the high-concentrate group were higher than the control group, but there had no significant different (P>0.05).

As shown in Figure 4B, the mRNA expression of IGF-1R of mammary gland in the high-concentrate group was significantly lower than the control group (P<0.05), while the mRNA expression of GH was higher than the control group.

Discussion

GH is one of the most important hormones which participate in ruminant mammogenesis, onset and maintenance of milk secretion. A large number of studies indicated that GH has a galactopoietics effect (Tucker, 2000). With respect to animal agriculture, administration of exogenous somatotropin (ST) is a biotechnology that increases the food output (meat or milk) per unit of feed resource input (Ferreira et al., 2021). Pocius and Herbein (1986) demonstrated that milk yield increased up to 40% with no adverse effects in the treated cows which were injected with bovine growth hormone lasting 10-12 weeks (Pocius and Herbein,1986). In 1985, the first longer term study reported that there was sufficient recombinant bst for 188 days of treatment, and impressive increases in milk yield and productive efficiency were observed (Bauman et al., 1985). Milk yield response to bst treatment has been observed in cows of all parities, but the magnitude of the increase in milk yield varies according to the stage of lactation. Typical milk yield responses are increase by 10-15% (4-6kg/day), although even greater increases occur when the management and care of the animal are excellent (Tucker, 2000). In our experiment, we observed that the content of plasma GH in the high-concentrate group (the forage to concentrate ratio was 40:60) was lower than the control group (the forage to concentrate ratio was 60:40). Scilicet the release of endogenous GH in the control group was more than the high-concentrate group. Considering the changes of milk lactose and milk yield, this finding was justified, because prior studies had demonstrated that milk yield would increase when the content of GH increased (Pocius and Herbein, 1986), indicating the endogenous GH has the same galactopoietic effect as exogenous GH.

The effect of increasing milk yield by adding exogenous GH has been reported by numerous studies, but the underlying mechanism is not fully understood. Most studies indicated that the galactopoietic effect of GH is indirectly mediated by the endocrine-, autocrine-, or paracrine-acting of IGF-1 (Belli et al., 2018). Cohick et al. (1989) demonstrated circulating concentrations of IGF-1 began to increase about 6-12 h after the bst injection and reached to a maximum concentration in approximately 48h (Cohick et al., 1989). Likewise, IGF-1 also stimulates DNA synthesis in mammary tissue cultures and thus may play a role in maintaining cell number during long-term ST treatment. Prosser et al found that infusion of IGF-1 into the pudenda artery of lactating goats for 6 h could increase milk production by about 30%. So IGF-1 plays a direct effect on promoting lactating and maintaining lactating. In our experiment, though detecting the content of plasma IGF-1, we found that compared to the control group, the content of plasma IGF-1 in the high-concentrate group was significantly lower. The result matched with the changes of plasma GH. Therefore, we thought that the increase of milk yield in control group was related to the higher level of endogenous GH, which promotes the release of IGF-1.

The somatomedin hypothesis suggested that GH exerts its effects by stimulating IGF-1 release from the liver, which in turn circulates to the target tissues (Isaksson et al., 2001; Kleinberg et al., 2011). The liver is the primary source for IGF-1 secretion in to the blood, and IGF-1 is transported to local tissues to exert its effects (Nguyen et al., 2013). A reasonable explanation is that IGF-1 binds to the IGF-1 receptor (IGF-1R) in mammary gland, which increases the activity and the proliferation of mammary epithelial cell, and ultimately promotes lactating (Nguyen et al., 2013). In our experiment, we found that the mRNA expression of IGF-1R in mammary gland was down-regulated in the high-concentrate group. The result was consistent to the results of milk yield, plasma GH and IGF-11. These results illustrated that the galactopoietics effect of endogenous GH was exerted by up-regulation of the IGF-1 and IGF-1R.

Lactose synthesis and milk yield have been found to be positively correlated with glucose uptake in the mammary gland of ruminants. It indicates that glucose uptake of mammary gland may be an important determining factor for the rate of milk synthesis (Shi et al., 2022). According to Baumruker, uptake of glucose and other nutrients in the mammary gland is determined by the supplement to the gland and the rate of transmembrane transport into the cell, however, supply is determined by the arterial nutrient concentration and mammary blood flow (Baumrucker, 1985). Bequette et al. (2001) has shown that milk yield and mammary blood flow are stimulated in goats when infused with IGF-1 from close-arterially into one mammary gland. Heo et al. (2017) found that IGF-1 increased both casein synthesis and glucose transport in the culture of mammary explants from mid-pregnant mice. A plausible mechanism may be that IGF-1 could promote glucose uptake in mammary gland by increasing mammary blood flow and glucose transport, and subsequently improves milk yield. In our study, we also found that the percentage of milk lactose in the high-concentrate group was lower than in the control group. We speculated that the galactopoietics effect of endogenous GH was related to the high level of lactose, which caused by IGF-1 promoting glucose uptake in the mammary gland.

We found that in the high-concentrate group, the mRNA expression of IGF-1R in the liver was higher than in the control group. But the result was contrary to the mRNA levels in the mammary gland. It is possible that the decreased endogenous GH caused by feeding with high-concentrate diet leads to the decrease of IGF-1, which generates in liver tissue. In mammary gland, IGF-1 binding to IGF-1R exerts its effects of promoting lactating and glucose uptake in the mammary gland. Therefore, glucose uptake in the mammary gland was attenuated when the content of IGF-1 decreased, and the milk yield was decreased. The reason for the induction of the mRNA expression of IGF-1R in the liver of high-concentrate group could be related to desensitizing protection, which caused by excess IGF-1 generated in liver. The detailed mechanism needs further study.

CONCLUSIONS

Numerous studies have found that milk quality and milk yield were decreased by long-term feeding with high-concentrate diets. Our study has demonstrated that in the high-concentrate diets feeding conditions, the content of endogenous GH was decreased, and then the content of IGF-1 was decreased possibly via the GH-IGF-1 axis. The mRNA expression of IGF-1R in mammary gland was down-regulated, leading to the weak action of glucose uptake in the mammary gland. It leads to the decreased lactose content and decreased milk yield.

Acknowledgments

We thank our colleagues from the Laboratory of Animal Physiology and Biochemistry for their assistance and comments that greatly improved the manuscript.

Funding

Financial support for this work was provided by Priority Academic Program Development of Jiangsu Higher Education and National Basic Research Program of China (Project No. 2011CB100802). This work was supported by the Hebei Youth Natural Science Foundation (C2020108002), Provincial Science and Technology Project of Hebei Province (22326616D), Scientific Research Project of Hebei Higher Education Institutions (BJK2024001), Xingtai Innovation Capability Plan Project (2022zz104).

IRB approval

The protocol for this study was reviewed and approved under project number 2011CB100802. The slaughter and sampling procedures strictly followed the `Guidelines on Ethical Treatment of Experimental Animals’ (2006) no. 398 created by the Ministry of Science and Technology in China as well as `Regulation regarding the Management and Treatment of Experimental Animals’ (2008) no. 45 from the Jiangsu Provincial People’s Government.

Ethical approval

The Institutional Animal Care and Use Committee of Nanjing Agricultural University (Nanjing, People’s Republic of China) approved all of the procedures (surgical procedures and care of goats).

Statement of conflict of interest

The authors have declared no conflict of interest.

References

Akers, R.M., 2006. Major advances associated with hormone and growth factor regulation of mammary growth and lactation in dairy cows. J. Dairy Sci., 89: 1222-1234. https://doi.org/10.3168/jds.S0022-0302(06)72191-9

Bauman, D.E., Eppard, P.J., DeGeeter, M.J. and Lanza, G.M., 1985. Responses of high-producing dairy cows to long-term treatment with pituitary somatotropin and recombinant somatotropin. J. Dairy Sci., 68: 1352-1362. https://doi.org/10.3168/jds.S0022-0302(85)80972-3

Baumrucker, C.R., 1985. Amino acid transport systems in bovine mammary tissue. J. Dairy Sci., 68: 2436-2451. https://doi.org/10.3168/jds.S0022-0302(85)81119-X

Belli, A.L., Reis, R.B., Veronese, A., Moreira, R., Flanagan, K., Driver, J., Nelson, C.D., Clapper, J.A., Ballou, M.A., Jeong, K.C. and Chebel, R.C., 2018. Effects of treatment of preweaning dairy calves with recombinant bovine somatotropin on immune responses and somatotropic axis. J. Dairy Sci., 101: 6602-6615. https://doi.org/10.3168/jds.2017-13917

Bequette, B.J., Kyle, C.E., Crompton, L.A., Buchan, V. and Hanigan, M.D., 2001. Insulin regulates milk production and mammary gland and hind-leg amino acid fluxes and blood flow in lactating goats. J. Dairy Sci., 84: 241-255. https://doi.org/10.3168/jds.S0022-0302(01)74474-8

Berryman, D.E., Chistiansen, J.S., Johannsson, G., Thorner, M.O. and Kopchick, J.J., 2008. Role of the GH/IGF-1 axis in lifespan and healthspan: Lessons from animal models. Growth Horm. IGF Res., 18: 455-471. https://doi.org/10.1016/j.ghir.2008.05.005

Cohick, W.S., Plaut, K., Sechen, S.J. and Bauman, D.E., 1989. Temporal pattern of insulin-like growth factor-I response to exogenous bovine somatotropin in lactating cows. Domest. Anim. Endocrinol., 6: 263-273. https://doi.org/10.1016/0739-7240(89)90020-9

Ferreira, ACG., Teixeira, RMA., Mendes, B.P., Vaneli, NR., de Oliveira, L.F., Silva, PSD. and de Almeida Neto, O.B., 2021. Effects of bovine somatotropin on productive performance and Minas Padrão cheese. Trop. Anim. Hlth. Prod., 53: 519. https://doi.org/10.1007/s11250-021-02968-w

Heo, Y.T., Ham, W.T., Lee, R., Lee, W.Y., Jeong, H.Y., Hwang, K.C. and Song, H., 2017. Mammary alveolar cell as in vitro evaluation system for casein gene expression involved in glucose level. Asian. Australas. J. Anim. Sci., 30: 878-885. https://doi.org/10.5713/ajas.16.0515

Isaksson, O., Ohlsson, C., Sjögren, K., Wallenius, K. and Jansson, J.O., 2001. The somatomedin hypothesis revisited in a transgenic model. Growth Horm. IGF Res., 11: S49-52. https://doi.org/10.1016/S1096-6374(01)80008-3

Kleinberg, D.L. and Barcellos-Hoff, M.H., 2011. The pivotal role of insulin-like growth factor I in normal mammary development. Endocrinol. Metab. Clin. North Am., 40: 461-471. https://doi.org/10.1016/j.ecl.2011.06.001

Ma, N., Abaker, J.A., Wei, G., Chen, H., Shen, X. and Chang, G., 2022. A high-concentrate diet induces an inflammatory response and oxidative stress and depresses milk fat synthesis in the mammary gland of dairy cows. J. Dairy Sci., 105: 5493-5505. https://doi.org/10.3168/jds.2021-21066

McCoard, S.A., Hayashi, A.A., Sciascia, Q., Rounce, J., Sinclair, B., McNabb, W.C. and Roy, N.C., 2016. Mammary transcriptome analysis of lactating dairy cows following administration of bovine growth hormone. Animal, 10: 2008-2017. https://doi.org/10.1017/S1751731116000987

Min, B.R., Hart, S.P., Sahlu, T. and Satter, L.D., 2005. The effect of diets on milk production and composition, and on lactation curves in pastured dairy goats. J. Dairy Sci., 88: 2604-2615. https://doi.org/10.3168/jds.S0022-0302(05)72937-4

Nguyen, D.V., Li, Calzi. S., Shaw, L.C., Kielczewski, J.L., Korah, H.E. and Grant, M.B., 2013. An ocular view of the IGF-IGFBP system. Growth Horm. IGF Res., 23: 45-52. https://doi.org/10.1016/j.ghir.2013.03.001

Pocius, P.A. and Herbein, J.H., 1986. Effects of in vivo administration of growth hormone on milk production and in vitro hepatic metabolism in dairy cattle. J. Dairy Sci., 69: 713-720. https://doi.org/10.3168/jds.S0022-0302(86)80460-X

Shi, H., Jiang, N., Wei, L., Cai, J., Zhang, W., Jiang, Q., Loor, J.J. and Liu, J., 2022. AMPK-ChEBP axis mediates de novo milk fatty acid synthesis promoted by glucose in the mammary gland of lactating goats. Anim. Nutr., 10: 234-242. https://doi.org/10.1016/j.aninu.2022.05.003

Tarazon-Herrera, M.A., Huber, J.T., Santos, J.E. and Nussio, L.G., 2000. Effects of bovine somatotropin on milk yield and composition in Holstein cows in advanced lactation fed low- or high-energy diets. J. Dairy Sci., 83: 430-434. https://doi.org/10.3168/jds.S0022-0302(00)74899-5

Tucker, H.A., 2000. Hormones, mammary growth, and lactation: A 41-year perspective. J. Dairy Sci., 83: 874-884. https://doi.org/10.3168/jds.S0022-0302(00)74951-4