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HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes

HSPC117 Improves the Rate of Embryonic Development by Up-Regulating the Transcription of Proto-Oncogenes

Hong Ma*, Bo Fu, Liang Wang, Zhong-qiu Li and Di Liu*

 

Heilongjiang Academy of Agriculture Science, Harbin 150086, P.R. China

*      Corresponding author: [email protected]; [email protected]

 

Fig. 1.

Identification of positive cell lines. A, HSPC117-transfected porcine fetal fibroblast cells line; B, HSPC117-transfected cell line confirmed using three differential size specific primer pairs.

Fig. 2.

Relative HSPC117 mRNA levels of cell clones. A, detection of HSPC117 mRNA expression in positive cell clones; B, electrophoresis of HSPC117 PCR product of cells.

Fig. 3.

Early embryonic development. A, HSPC117 transgenic embryo; B, pcDNA3.1 embryo (control); C, HSPC117 mRNA relative expression level.

Fig. 4.

Relative expression levels of c-Fos, c-Jun, Raf-1, c-Myc and control mRNA in HSPC117-transfected reconstructed blastocysts.

Pakistan Journal of Zoology

November

Pakistan J. Zool., Vol. 56

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