Histopathological and Cytogenetic Effects of Hyperdoses of Vitamin D in Female Rats
Histopathological and Cytogenetic Effects of Hyperdoses of Vitamin D in Female Rats
Ghaith Z. Hasan Al-Askari *, Eman H. Yousif Al-Taee
ABSTRACT
The Highlights of the present study was undertaken to investigate the toxic effects of the higher dose group (HG) (549.640 IU, i.e., 1.3741 mg/kg body weight), which represent 1/10 LD50, and the lower dose group (LG) (274.820 IU, i.e., 0.6875 mg/kg body weight), which represents 1/20 LD50 of vitamin D3. So, the objectives of this rapid review were to assess the median lethal dose (LD50) of VD in female albino rats and to assess the effects of vitamin D (VD) on the severity of acute upper or chronic vitamin D intoxication (VDI) through the study of cytogenetic effects in addition to histopathological changes in the tissues of female rats. The methods included in the first experiment, the female rats were orally administered the following lethal doses: 0.5, 1, 1.5, 2, 2.5, 3, 4, 5, 6, 7, 8, 9, 10, 11, 12, 13, 14, 15, and 16 mg/kg b.w. of vitamin D. Survival and mortality numbers were observed following 24 hours of intoxication. In the second experiment, the selected groups were divided according to the doses they received. In a cross-sectional study, 30 female rats were divided into three groups: a control group (CG: 0 mg/kg VD), a high VD group (HG: 1/10 LD50), and a low VD group (LG: 1/20 LD50). While the Results included at the dose of 1/10 LD50, the lesions were more severe than the lesions at 1/20 LD50. The characteristic histological finding in the liver is severe and generalized hepatocellular necrosis, which revealed congestion, hemorrhage, and degeneration of hepatic cells. Some sections of the kidneys show vacuolation of mesangial cells in the glomerular tuft. Muscles of treated rats revealed severe histopathological changes under stress from both doses of toxic cholecalciferol, also showing the widespread mineralization of muscle fibers. From this study concluded that both doses of vitamin D can be toxic to organisms at higher levels, so they need to be used carefully and should not be taken excessively.
Keywords | Hypervitaminosis D, Chromosome gap, Histopathological, Cytogenetic effects, Vitamin D, Median lethal dose, Mitotic index, Rats, Female rats.
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April 2024
Vol. 12, Iss. 4, pp. 586-801
