Identification of Reproductive Performance in Landrace Boar: The Sperm Quality and Molecular Weight of Protein
Identification of Reproductive Performance in Landrace Boar: The Sperm Quality and Molecular Weight of Protein
Wilmienthe Marlene Mesang Nalley1*, Thomas Mata Hine1, Aloysius Marawali1, Solvi Mariana Makandolu1, Maria Marsefar Raja Kota1, Athhar Manabi Diansyah2, Annisa Rahmi3, Abdullah Baharun3
ABSTRACT
The study aimed to investigate the role of specific proteins in improving the quality of Landrace boar semen, focusing on how these proteins may influence semen characteristics related to reproductive performance. Using the 1D-SDS-PAGE technique, relevant proteins were separated and identified. Semen from six Landrace boars was evaluated macroscopically (volume, pH) and microscopically (concentration, motility, viability, and abnormality), while protein content was analyzed using 1D-SDS-PAGE. Statistical analysis using ANOVA revealed variations in semen quality across the boars. Notably, semen volume in ID6 was significantly lower (p < 0.05) compared to the others, though pH did not significantly differ (p > 0.05) across all boars. Similarly, no significant differences (p > 0.05) were found in sperm motility, concentration, viability, or abnormality. In frozen semen, ID1 had significantly higher sperm motility and viability (p < 0.05) than ID5 and ID6, suggesting differences in preservation potential. Additionally, frozen semen production potential was highest in boars ID1, ID2, and ID6. Proteomic analysis identified three common protein bands in the semen plasma, with molecular weights (MW) of 10-15 kDa, 75-100 kDa, and 250 kDa, and in sperm, with MWs of 10-15 kDa, 50 kDa, and 75 kDa. The pearson’s correlation showed a strong positive correlation (p < 0.05) was found between sperm motility and MWSP, and between sperm viability and MWSP, particularly in ID5 and ID6. These findings suggest that the variation in sperm quality across Landrace boars is associated with the presence of critical proteins, which can significantly impact reproductive performance. Proteins such as OPN12, HSP90, and GRP94, identified in the analysis, are known to play key roles in sperm motility, viability, and fertility, offering insights into their influence on sperm quality and infertility in Landrace boars.
Keywords | Landrace boar, Sperm quality, 1D-SDS-PAGE, Protein, Molecular weight, Reproductive performance
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