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Isolation and Molecular Identification of Enterotoxigenic Strains of Escherichia coli in Raw and Pasteurized Milk

Isolation and Molecular Identification of Enterotoxigenic Strains of Escherichia coli in Raw and Pasteurized Milk

Rahman Ullah1*, Muhammad Junaid2, Nabila Gulzar2, Rahat Ullah Khan3, Baseer Ahmad4, Ambrina Tariq5, Aamir Iqbal6, Mushtaq Ahmed7 and Mirwaise Khan8*

1Faculty of Veterinary and Animal Sciences, The University of Agriculture, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan; 2Department of Dairy Technology, University of Veterinary and Animal Sciences, Lahore, Pakistan; 3Institute of Microbiology, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan; 4Faculty of Veterinary and Animal Sciences, Muhammad Nawaz Sharif University of Agriculture, Multan, Pakistan; 5Directorate of Livestock and Dairy Development Department, Khyber Pakhtunkhwa, Pakistan; 6Department of Animal Nutrition, Faculty of Veterinary and Animal Sciences, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan; 7Livestock and Dairy Development Department, Quetta, Government of Balochistan, Pakistan; 8Department of Clinical Sciences, Faculty of Veterinary and Animal Sciences, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan.

 
*Correspondence | Mirwaise Khan, Gomal University, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan; Email: drmirwaise2321@gmail.com & Rahman Ullah,  The University of Agriculture, Dera Ismail Khan, Khyber Pakhtunkhwa, Pakistan; Email: rahmanmohmand99@yahoo.com 

Figure 1:

PCR amplified result (gel electrophoresis) for LT-I in all E. coli positive samples.

Figure 2:

PCR amplified result (gel electrophoresis) for LT-II in all E. coli positive samples.

Figure 3:

PCR amplified result (gel electrophoresis) for ST-I in all E. coli positive samples.

Figure 4:

PCR amplified result (gel electrophoresis) for ST-II in all E. coli positive samples.

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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