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Isolation and Complete Capsid Sequence of Enterovirus D111 from Faeces of a Child with Acute Flaccid Paralysis in Nigeria

Isolation and Complete Capsid Sequence of Enterovirus D111 from Faeces of a Child with Acute Flaccid Paralysis in Nigeria

Faleye Temitope Oluwasegun Cephas1,2*, Adewumi Moses Olubusuyi1, Olayinka Olaitan Titilola3 and Adeniji Johnson Adekunle1,3 

1Department of Virology, University of Ibadan, Ibadan, Oyo State, Nigeria; 2Center for Human Virology and Genomics, Department of Microbiology, Nigerian Institute of Medical Research, Lagos, Nigeria; 3WHO National Poliovirus Laboratory, University of Ibadan, Ibadan, Oyo State, Nigeria.

[email protected] 

Figure 1

A schematic representation of the algorithm followed in this study. 

Figure 2

The Contigs assembled from the Illumina sequencing data generated for the three PE-5UTR positive isolates. The contigs and the genomic regions covered are displayed using the EGT. 

Figure 3

All the EV-D111 sequences in GenBank described till date alongside a reference EV-D70 and the EGT identification glitch. 

Figure 4

Phylogram of partial VP1 sequences of EV-D111. The phylogenetic tree is based on an alignment of partial EV-D111 VP1 sequences available in GenBank and the one described in this study. The EV-D111 described in this study is indicated with a black triangle. Bootstrap values are indicated if >50%.  

Figure 5

Global distribution of EV-D111 detection sources. It is crucial to note that, till date, all have been detected in sub-Saharan Africa. 

Hosts and Viruses

December

Vol.11, Pages 01-115

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