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Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq

Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq

Anwar A. Maki* and Asaad M.R. Al-Taee

Department of Biological Development, Marine Science Center, University of Basrah, Basra, Iraq.

 
*Correspondence | Anwar A. Maki, Department of Biological Development, Marine Science Center, University of Basrah, Basra, Iraq; Email: [email protected] 

Figure 1:

Pink pigment facultative methylotrophic bacteria on MSM medium supplemented with 1% methanol as a sole source of carbon.

Figure 2:

Agarose gel electrophoresis of the amplified MxaF gene at 60 volts for 60 min. Lane 1: 100 bp ladder; Lane 2: band of the MxaF gene in the selected Methylorubrum pseudosasae (detected as a single band of 550 bp); Lane 3: negative control.

Figure 3:

Growth of Methylorubrum pseudosasae strain AAZ2 in MSM medium supplemented with 1 g of crude oil after 10 d of incubation.

Figure 4:

Gas chromatography (GC) of n-alkane for crude oil as a control (a) and of n-alkane obtained from culture fluids of Methylorubrum pseudosasae strain AAZ2 after 5 d (b), and 10 d (c) of incubation at 30 °C.

Figure 5:

Gas chromatography of PAH compounds for crude oil as a control (a) and PAH compound from the culture fluids of the Methylorubrum pseudosasae strain AAZ2 after 5 d (b) and 10 d (c) of incubation at 30 °C.

Pakistan Journal of Zoology

November

Pakistan J. Zool., Vol. 56

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