Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq
Isolation and Identification of Pink-Pigmented Facultative Methylotrophic Bacteria (PPFM) from the North Rumaila Field, Iraq
Anwar A. Maki* and Asaad M.R. Al-Taee
Pink pigment facultative methylotrophic bacteria on MSM medium supplemented with 1% methanol as a sole source of carbon.
Agarose gel electrophoresis of the amplified MxaF gene at 60 volts for 60 min. Lane 1: 100 bp ladder; Lane 2: band of the MxaF gene in the selected Methylorubrum pseudosasae (detected as a single band of 550 bp); Lane 3: negative control.
Growth of Methylorubrum pseudosasae strain AAZ2 in MSM medium supplemented with 1 g of crude oil after 10 d of incubation.
Gas chromatography (GC) of n-alkane for crude oil as a control (a) and of n-alkane obtained from culture fluids of Methylorubrum pseudosasae strain AAZ2 after 5 d (b), and 10 d (c) of incubation at 30 °C.
Gas chromatography of PAH compounds for crude oil as a control (a) and PAH compound from the culture fluids of the Methylorubrum pseudosasae strain AAZ2 after 5 d (b) and 10 d (c) of incubation at 30 °C.