Lemongrass Extract and Anticancer Impact: mRNA Levels of Expressing Apoptosis and Mitochondrial Fission in MCF7 Cells
Lemongrass Extract and Anticancer Impact: mRNA Levels of Expressing Apoptosis and Mitochondrial Fission in MCF7 Cells
Mashael Alhumaidi Alotaibi*
Cytotoxicity assessment by MTT assay on MCF7 breast cancer cells exposed to various concentrations of lemongrass leaves extract for 24 h. Stars indicate statistically significant differences of cytotoxicity and cell viability assessment between the concentrations (0, 50, 100, 200 and 300 μg/mL). Values are put as percentages of the control (mean ± SEM, n = 5) ***P < 0.001, **P < 0.01, *P < 0.05 in comparison to the control (0 μg/mL).
The effect of lemongrass extract on MCF7 cells showing abnormal shape of nuclei by (Hochest 33342-blue stain) and mitochondrial fusion by (100 nM MitoView™ 405- Blue stain). A, represents untreated MCF7 cells with normal structure and undamaged mitochondrial status. In contrast, other MCF7 cells were treated by lemongrass extract had mitochondrial changes in shape with fragmented patterns and punctiform morphology when MCF7 cells treated at four different concentrations (50, 100, 150 and 200 µg/mL) as seen in B, C, D, and E, respectively.
