Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

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Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Saba Mehnaz¹*, Farhan Ahmad Atif², Rao Zahid Abbas¹, Muhammad Kasib Khan¹ and Muhammad Saqib³

Author Affiliations

¹Department of Parasitology, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.

²Medicine Section, Department of Clinical Sciences, College of Veterinary and Animal Sciences, Jhang (Sub-Campus), University of Veterinary and Animal Sciences, Lahore, Pakistan.

³Department of Clinical Medicine and Surgery, Faculty of Veterinary Science, University of Agriculture, Faisalabad, Pakistan.

* Corresponding author: [email protected], [email protected]

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Fig. 1.

District wise prevalence of Anaplasma marginale and Theileria annulate in study districts.

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Fig. 2.

Phylogenetic analysis of Anaplasma marginale based on msp1β gene. Our revealed sequences are indicated with Pak-3* and Pak-4*.

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Fig. 3.

Phylogenetic analysis of Theileria annulate based on cytochrome b gene. Our revealed sequences are indicated with Pak-2*.

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Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Saba Mehnaz¹*, Farhan Ahmad Atif², Rao Zahid Abbas¹, Muhammad Kasib Khan¹ and Muhammad Saqib³

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Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Molecular Detection of Anaplasma marginale and Theileria annulata in Buffaloes by Using Duplex PCR from Sheikhupura, Jhang, and Rawalpindi Districts of Punjab, Pakistan

Saba Mehnaz1*, Farhan Ahmad Atif2, Rao Zahid Abbas1, Muhammad Kasib Khan1 and Muhammad Saqib3

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Saba Mehnaz¹*, Farhan Ahmad Atif², Rao Zahid Abbas¹, Muhammad Kasib Khan¹ and Muhammad Saqib³