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Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City

Molecular Detection of Leucocytozoon spp. in Broiler Chickens in Baghdad City

Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2

1Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq; 2Department of Parasitology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq. 

 
*Correspondence | Rana Mohammed Ibrahim, Department of Microbiology, College of Veterinary Medicine, University of Baghdad, Baghdad, Iraq; Email: [email protected]

ABSTRACT

The purpose of this study was to detect the infection by Leucocytozoon spp. parasite in broiler chickens by using thin blood smears Nested and quantitative real time (qRT)- Polymerase Chain Reaction methods in Baghdad city between 1/11/ 2021 and 31/3/2022. Fifty jugular venous blood samples (about 5ml) were collected. The total infection rate of Leucocytozoon spp. in the blood smears was 10% (5/50), which divided into males 6% and in females 4%, while by qRT-PCR was 4% (2/50) with an equal ratio between males and females (2%). In conclusion, it is the first diagnosed study in Baghdad city to detect Leucocytozoon spp. in broiler chickens by using qRT– PCR. 
 
 
Keywords | Leucocytozoon, Broiler, Chickens, q RT–PCR, Haemosporidian, Avian, Birds

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Advances in Animal and Veterinary Sciences

March

Adv. Anim. Vet. Sci., Vol. 13, Iss. 3,

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