Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...n breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible association with egg production performances including age at first egg (AFE), laying rate (LR), egg production (EP), egg weight (EW)...
Gabriela Mansano do Nascimento, Helena Lage Ferreira and Clarice Weis Arns
...ressive disease in young chickens. The first line of defence against the virus is innate immunity whose activation is independent of the antigen and depends on the ability of the host to recognize pathogens by means of specific pattern recognition receptors. This interaction induces the production of cytokines, chemokines and interferons. Through Janus kinase/signal transducers and transcription activators pathway, interferon induces the transcription of inter...
Hui Zhang2, Yajing Wang2, Kun Li2, Mujeeb Ur Rehman2, Fazul Nabi2, Rui Gui2, Yanfang Lan2 and Houqiang Luo1,2*
...ant neoplasm in domestic chickens with subsequent economic losses to poultry industry. Effected birds show lymphocyte tumor, abnormal proliferation and series of tumors in connective tissues and other visceral organs. To evaluate the status of lymphoid leukosis virussubgroup A (ALV-A) infection in free-range chickens in five different cities of Anhui province, a total of 732 serum samples were analyzed through enzyme-linked ...
Wesam Hasan Mohamed Mady 1*, Bing Liu2, Dong Huang2, A. Arafa1, M.K. Hassan1, M.M. Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...ular immunization of SPF chickens with different concentrations of plasmid DNA and the sera were collected weekly post vaccination for antibody detection by HI test. All immunized chickens shown high HI antibody titers (9Log2) two weeks post-booster dose. The chickens were then challenged using homogenous AI H9N2 strain. During the course of infections, oropharyngeal and cloacal swabs were...
Vladimir Avdalovic1, Marijana Vucinic2, Radmila Resanovic3, Jelena Avdalovic4, Danka Maslic-Strizak5 and Milos Vucicevic3*
...matitis (FPD) in broiler chickens. The study included 300 one-day-old Ross 308 broilers. One group of broilers was reared on chopped wheat straw (the particle length of straw ≥ 10 cm). The second group was kept on newly formulated pelleted wheat straw. The duration of the trial was 42 days. Moisture content in litter materials and FPD occurrence and severity were assessed weekly. First signs of FPD were observed in 21-day old chicke...
Wesam Hasan Mohamed Mady1*, Bing Liu2, Dong Huang2, Abdel Satar Arafa1, Mohamed Khalifa Hassan1, Mona Mehrez Aly1, Pucheng Chen2, Yongping Jiang2* and Hualan Chen2
...ific pathogen free (SPF) chickens with two different concentrations of Egy-H5 plasmid DNA 15µg and 60 µg. Results demonstrate that chickens developed detectable HI antibody titers up to 6log2 within two weeks post-booster vaccination. The challenge experiment was performed using isolate A/chicken/Egypt/A6/2011 (H5N1) with concentration of 105 EID50 to evaluate the protective efficacy of the Egy-H5 plasmid DNA vac...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool3, Fatima Ahmed4, Saiqa Tabassum3,6, Syeda Madiha3, Tahira Perveen3 and Saad Bilal Ahmed5
...he increasing demand the chickens are reared commercially through feeds and battery cages allowing maximum weight gain and growth in considerably less time. The constituents that are fed to chickens concentrate in the flesh and bring deleterious health outcomes on the consumers. The ill effects account for hyper-lipidemias and imbalance in the steroidal sex hormones may result in the development of cysts in the ovaries with ...
Alaeldein M. Abudabos*, Abdullah N. Al-Owaimer, Elsayed O.S. Hussein and Mutahar H. Ali
Zhiping Hu, Hussain Ahmad, Jingfei Zhang, Lili Zhang and Tian Wang*
Yan Li1, Kai Zhan1,*, Junying Li1, Wei Liu1, Ruiyu Ma1, Shengnan Liu1, Tao Han2, Shaoquan Li2, Shaolin Wang2 and Yang Hu2
...hering Huainan partridge chickens. A total number of 360 fast feathering Huainan partridge hens and 40 roosters were randomly divided into two equal groups. For NM, 4 mating cages were maintained each with 5 roosters and 45 laying hens. The other birds were reared in 3-layer complete ladder cages for AI, for which 180 hens were maintained in 60 cages with 3 hens/cage and 20 roosters in 20 cages. Laying performances, mortality, egg quality characteristics and w...
Evolutionary Analysis and Phylodynamics of Avian Influenza Virus H5N1 between 2015 and 2016 in Egypt
Osama Elshazly1, AbdelSatar Arafa1, Mohammed A. Rohaim2, Ismaeil M. Reda2 and Hussein A. Hussein2*
...isolates from humans and chickens. Our results revealed that linking the epidemiological and sequence data is important to understand the prevalence, transmission, persistence and evolution of the virus, and to monitor the circulating AIV strains and emergence of new AIV subtypes in Egypt. The wide circulation of the 2.2.1.2 subclade, carrying triple mutations (120, 129∆, I151T) associated with increased binding affinity to human receptors, is an alarmin...
Nashwa Mohamed Eid1, Al-Hussien Mohamed Dahshan1, El-Shayma El-Nahass2, Basma Shalaby3 and Ahmed Ali1*
...nteritis (NE) in broiler chickens. The influence of varying concentrations of both EOs was determined in vitro using the agar dilution test. Concentration as low as 0.25% and 0.125% of thyme and clove oils, respectively, were able to completely inhibit the growth of CP. An in vivo study was conducted in commercial broiler chickens, where, 2 groups of 10 day-old broilers were fed a balanced ration with addition of thyme (0.25...
Fazul Nabi1,2, Hui Zhang1, Muhammad Kashif Iqbal1 , Mujeeb ur Rehman1, Muhammad Shahzad3, Khalid Mehmood1,3 and Jiakui Li1,*
...contents in TD afflicted chickens compared to control group. The SM administration to TD affected birds significantly ameliorated lameness, stimulated ALP level with a decrease in ALT and AST contents, increase in antioxidant parameter and decrease in MDA contents significantly (P<0.05). SM treatment of TD-afflicted birds prevented lameness and reinstated antioxidant imbalance. SM may be effective for the treatment and control of TD and minimizing the liver...
Abdul Wajid1,*, Asma Basharat2, Muhammad Akbar Shahid3, Sidra Tul Muntaha1, Abdul Basit4, Tanveer Hussain5, Muhammad Farooq Tahir6, Muhammad Azhar7, Masroor Ellahi Babar1 and Shafqat Fatima Rehmani2
...ardium syndrome (HPS) in chickens worldwide. Identification of fowl adenovirus (FAdV) serotype is of most importance in epidemiological studies of disease outbreak and the adaptation of vaccine strategies. In spite of appropriate administration of vaccination, FAdV outbreaks have been reported and caused significant losses in poultry flocks throughout Pakistan in recent years. To identify the serotype and gain a better understanding of the genetic properties o...
Ali Karabayir1,*, Nazan Arifoğlu2 and Mustafa Öğütcü3
...(local mix-breed village chickens) was evaluated in the present study. Supplementation of disinfectant clearly improved the microbiological quality of chicken drinking water, by reducing the total bacteria, coliform and E.coli counts. On the other hand, disinfectant addition decreased feed and water intake of all chicken strains. Furthermore, the results revealed that feed and water intake was strain dependent. Lower feed conversion ratio was observed f...
Mushtaq Hussain Lashari1*, Fozia Afzal1 and Umer Farooq2
...es(%) values of negative chickens were 9.43±0.49 g/dl,, 25.81±1.34%, 1.84±0.13 x106/µL, 151.7±11.0 fl, 3.85±0.22 pg, 39.34±2.07%, 3.98±0.13 x103/µL, 45.71±1.26%, 2.08±0.29%, 1.54±0.17%, 2.58±0.29% and 48.10±1.36% and for positive chickens were 9.35±0.89 g/dl, 24.63±2.21%, 1.68±0.17 x106/µL, 168.9±...
Kalimullah Khan1,*, Hafsa Zaneb2, Zia Ur Rehman3, Hamza Maris4 and Habib ur Rehman1
Ahmed Samy1*, Hala Mohamed Nabil Tolba2, Gamelat Kotb Farag3 and Ahmed Abd El Halim1
...n but also it can infect chickens and its virulence enhanced upon passaging in chickens causing clinical signs and deaths. In the present study brain samples were collected from twelve pigeon flocks exhibiting signs of nervousness and diarrhea in a live bird market (LVM) in Egypt. All collected samples were positive for NDV and negative for avian influenza virus and infectious bronchitis virus. Seven positive samples were su...
Ali Raza Jahejo1,2, Nasir Rajput2, Wen-xia Tian1,*, Muhammad Naeem2, Dildar Hussain Kalhoro2, Asmatullah Kaka2, Sheng Niu1 and Fa-jie Jia1
Hande Küçükönder1, Pınar Çelebi Demirarslan1,*, Aykut Burgut2 and Mustafa Boğa3
... Lohman Brown genus 8000 chickens. According to the findings of the analysis, it was seen that January, March, October, November and December are the months when production performance is high. When these months were ranked among themselves, it was observed that January, March and November are the first three ranks, respectively, and that the rank was also supported by the combined results of different techniques. As a result, in terms of production performanc...
Emel Banu Buyukunal1,*, Rojan Ibrahim Albazaz1 and Mehmet Ali Bal2,3
...3 Japanase quails and 22 chickens were analyzed by conventional and molecular methods for E. coli isolation in this study. Subsequently, antimicrobial susceptibility profiles of E. coli isolates (n=71) were determined against thirteen agents; amoxicillin-clavulanic acid, piperacillin-tazobactam, ceftazidime, cefotaxime, ceftriaxone, cefpodoxime, aztreonam, ciprofloxacin, amikacin, ertapenem, imipenem, meropenem, trimethoprim-sulfamethoxazole by d...
Sheng Niu1, Ali-Raza Jahejo1, Fa-jie Jia1, Xin Li1, Guan-bao Ning1, Ding Zhang1, Hai-li Ma1, Wei-fang Hao2, Wen-wei Gao1, Yu-jun Zhao1, Shi-min Gao1, Jian-hui Li1, Gui-lan Li1, Fang Yan1, Rong-kun Gao1, Huan-chun Chen1,3 and Wen-xia Tian1,*
...ondroplasia (TD) and the chickens were injected with different doses of recombinant glutathione-S-transferase A3 (rGSTA3) proteins. We explored the responses of HDPs in erythrocytes to thiram-induced TD and rGSTA3 protein by quantitative real time PCR (qRT-PCR). The results showed many HDPs expressions were suppressed by thiram-induced TD, and the expressions of HDPs were upregulated by rGSTA3 protein. These findings demonstrated that mRNA expressions of HDPs ...
Chen-Yu Sun, Wen-Chao Liu*, Mei Xiao, Zhi-Hui Zhao and Li-Long An*
...ale, one-d-old Huaixiang chickens with an average initial BW of 44.52 ± 1.07 g were randomly allotted to 4 treatments. Each dietary treatment consisted of 6 replicate cages, with 15 birds per replicate. The 4 dietary treatments were corn-soybean meal-based diets and supplemented with 0, 500, 1000 and 2000 mg/kg betaine, respectively. During d 1-14, with increasing the dietary betaine levels from 0 to 2000 mg/kg, the BWG was improved (quadratic, P<0.0...
Tahir Iqbal1, Umer Rashid1*, Naveed Shahzad2, Amber Afroz1, Muhammad Faheem Malik3 and Muhammad Idrees4
...megaly syndrome (HSS) in chickens. To the best of our knowledge the circulation of aHEV in chicken has not been studied in Pakistan so far. Therefore, in the present study, we aimed to isolate and identify aHEV from layer chickens of Pakistan. The bile fluids, liver and spleen tissues were collected from overnight dead layer chickens (n = 8) from Pattoki region of Punjab province, Pakistan...
Zia-ud-Din1, Muhammad Nauman Aftab2, Tanveer Hussain3,*, Irfana Iqbal4 and Asma Zafar5
...rieties (P<0.05). The chickens given feed with probiotic (@1g/kg of feed were of more economic value than those fed on antibiotic supplemented feed and control with highest live body weight of 183.58±6.31g gaining 143.13±6.37g weight, multiplying their weight 4.56±0.17 times over the initial body weight with feed intake of 668.82±31.11g showing most efficient FCR (4.67±0.25) with least mortality and highest net profit (Rs....
Hande Kucukonder1, Pinar Celebi Demirarslan1*, Sezai Alkan2 and Özgür Baris Bırgul3
...broiler growth curves of chickens with nonlinear regression analysis and grey prediction model. For this, the growth of 118 broilers was analyzed by using their weekly individual live weights from hatch to 49 day-old. In the analysis, nonlinear functions and Rolling-Grey Model (1,1) prediction method were used. The time-dependent growths of mixed sexes broilers were analyzed in the aspects of testing the parallelism of female and male growth samples, determini...
Shwe Yee Win1, Lat Lat Htun1, Myint Myint Hmoon1, Hla Myet Chel1, Yu Nandi Thaw1, Nyein Chan Soe1, Htet Lin Oo3 and Saw Bawm2*
...nal parasites in village chickens from four Townships (Mawlamyine, Nyaung U, Zay Yar Thi Ri and Katha) of Myanmar from April to June 2019. A total of 200 faecal samples were randomly collected from village chickens. Centrifugal sugar floatation and sedimentation methods were carried out to detect the gastrointestinal parasites. The overall occurrence of gastrointestinal parasites in village chickens<...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
.../i>Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, including three novel mutations [SNP4 (A4812316G), SNP6 (C4813363A) and SNP7 (C4813618T)] and five known loci, were found within 3292 bp sequenced fragments. The allele frequency and genotype frequency of SNP...
Saara Ahmad1,*, Iftikhar Ahmed2, Saida Haider3, Zehra Batool4, Laraib Liaquat3, Fatima Ahmed5, Asra Khan1, Tahira Perveen3, Mirza Jawad ul Hasnain6, Saima Khaliq7 and Saad Bilal Ahmed8
...n of commercial feed for chickens and their meat which is considered safe for human consumption.
...
Nahed H. Ghoneim, Maha A. Sabry, Zeinab S. Ahmed and Esraa A. Elshafiee*
...ontact with the examined chickens. Eleven Campylobacter isolates were recovered through bacteriological examination. Campylobacter spp. were identified by polymerase chain reaction (PCR) as C. jejuni (63.6 %) and C. coli (36.4 %) through the detection of the Map A and Ceu E genes, respectively. The antibiotic resistance of the Campylobacter isolates was determined via the disc diffusion method and was observed m...
Irfan Shahzad Sheikh, Masroor Ahmad Bajwa, Nadeem Rashid, Muhammad Zahid Mustafa, Mohammad Masood Tariq*, Majed Rafeeq, Abdul Samad, Tauseef M Asmat and Asad Ullah
...ne competence of broiler chickens. In three experiments (n=240 chicks in each), the immune modulators vitamin C (500 mg/l), vitamin E (200 mg/l), dietary nucleotides (100 mg/l) and DNA (100 mg/l) extracted from chicken liver were separately offered on days 1-21, 1-42 and 22-42 in experiments 1, 2 and 3, respectively. Under standard broiler management conditions, birds were divided into five treatment groups of 48 with four replicates. Commercial pre-starter fe...
Muhammad Saeed Imran1, Asim Aslam1*, Muhammad Yasin2, Tahir Yaqoob2 and Beenish Zahid3
...ine efficacy, commercial chickens were vaccinated with La Sota (against NDV) and Massachusetts (against IBV) vaccine preparations. After screening the protective titers in birds at 19 days post inoculation three week-old broilers were challenged with 10-5.76 and 10-6.03 EID50 dose of NDV and IBV, respectively. The hosts were divided into six groups of 35 birds each. These were groups A (NDV-challenged), B (IBV- challenged), C (...
Yan Zhou1,2, Hai Xia Han1,2, Qiu Xia Lei1,2, Jin Bo Gao1,2, Wei Liu1,2, Fu Wei Li1,2, Jie Liu1,2 and Ding Guo Cao1,2*
...VLDLR on reproduction in chickens, the haplotypes and diplotypes based on three genetic mutations (NC_006127.2:g.8467G>A, NC_006127.2:g.12321G>A and NC_006127.2:g.13876A>G) were constructed, and the associations of diplotypes with reproduction traits were assessed, their effects on gene expression were evaluated also. As a result, three haplotypes H1 (G-G-A), H2 (G-G-G) and H3 (A-A-G) were obtained, H1 was the main haplotype with a frequency of 91.75%...
Christian Marco Hadi Nugroho1 and Jeanne Adiwinata Pawitan2,3,4*
Production of Antibody for Direct Fluorescence Antibody Assay against Avian Influenza H9N2
...in unimmunized humans or chickens. In reducing losses due to H9N2 infection, rapid diagnosis is an option to prevent further infections. Office International des Epizooties (OIE) suggested some methods for the detection of H9N2 infection including the immunofluorescence method that is commonly known as the direct fluorescent antibody (DFA) assay. This method is a combination of immunology and bio-imaging. This DFA assay is rapid and sensitive, but specificity ...
Muhammad Faisal Ayoob1,2, Zaheer Ahmed Nizamani1, Asghar Ali Kamboh3, Mansoor Ayoob4, Waseem Ali Vistro3 and Abdul Sattar Baloch3*
...with VVND virus infected chickens. Clinical signs were observed in Japanese quails and mynas on 4th day post-inoculation. The major clinical signs were green yellowish diarrhea, ruffled feathers, anorexia and torticollis in all groups except Q3. The mean clinical scores and mean death time (days) were statistically non-significant (P>0.05) among all the groups. Higher mortality rate (75%) was recorded in mynas as compared to Japanese quails (50%)...
Iram Liaqat1*, Tahir Hussain1,2, Aisha Waheed Qurashi3, Gulbeena Saleem2, Asia Bibi4, Muhammad Fiaz Qamar5,ShaukatAli1 and Ikram-ul-Haq6
Natalija Grittner1, Radomir Mandić2,* and Milivoje Urošević3
...alo, sheep, goats, pigs, chickens, geese, turkeys, ducks, guinea fowls, dogs and bees were analyzed. The number of populations these indigenous breeds in Serbia has been shown in this paper. It is proposed that 15 breeds (13 breeds and 2 strains), which are not protected by the Decree of the Ministry of Agriculture of Serbia on animal genetic resources, be included in the Decree in the future, in order to preserve and improve their numbers. An urgent establish...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Ying Wang1, Ding Zhang1, Yu-hai Bi1,2, Ali Raza Jahejo1,Qian-qian Cheng1 and Wen-xia Tian1,*
...rocytes of H9N2 infected chickens. In current study, qRT-PCR and Western blot analysis were performed to identify the expression levels of PG-related genes (COX-2, GSTA3, PTGDS, PTGES, PTGER3 and PTGER4) in erythrocytes of H9N2 infected chickens at time intervals of 0, 2, 6 and 10 h and P < 0.05 was considered as statistically significant. Expression levels were increased significantly in case of COX-2 at 0 and 6 h...
Majed Rafeeq1,*, Nadeem Rashid1, Muhammad Masood Tariq1, Irfan Shahzad Sheikh1, Muhammad Zahid Mustafa1, Muhammad Shafee1, Khalid Mehmood1, Rana Muhammad Bilal2 and Tauseef Asmat1
...e performance of broiler chickens. The study used 120 unsexed Hubbard one day old broiler chicks randomly divided into three groups of 40 chicks that were each further divided into four replicates of ten chicks. Extracts were introduced at 200 mg/Kg as feed supplement to two groups of broilers and the third group was kept as control (C) and offered a basal diet. The chicks were reared in floor pens bedded with saw dust litter. Measured feed was offered twice a...
RehamM. El-Bakrey1, Shimaa M.G. Mansour2, Mohammed A. El Sisi1 AndAmal A.M. Eid1
...AI viruses isolated from chickens, Egypt during 2009-2011. Both inactivated and recombinant fowlpox virus expressing H5 (rFP-AI-H5) vaccines were measured. Vaccine efficacy was evaluated by percentage of protection from mortality, morbidity and reduction in virus shedding from respiratory and/orintestinal tracts. Despite the H5 antibody responses in vaccinated chickens with program I (rFP-AI-H5" vaccine then Re-5) being...
Afaf. A. Khedr*, Ekram Salama*, A. A. Samy**, Abdella, Y.A.*** and Suzan. K. Tolba***
...s bronchitis diseases in chickens....
Amir M. Walaa1 ; Ausama Yousif1; Walid H. Kilany2; Magdy El-Sayed1; Ahmed A. El-Sanousi1
...ect experimental broiler chickens against challenge with wild ND and IB viruses. Two hundred and eighty commercial one-day-old broiler saso chicks were divided into 8 groups (35 birds each). Groups 2, 3, 4&5 were vaccinated at one-day-old with live hitchner + IB Primer. Then all groups at 7 days old were boosted with inactivated combined NDV+IBV vaccines with different adjuvants except for group 1 & 2. Group 1 was kept as unvaccinated control. At 28 da...
Hanan M. El-Zahed, Hanaa A. Mostafa, Nermeen M. El Sayed and Lamiaa M. Omar
...of nine week old Hubbard chickens were vaccinated with two different commercial live attenuated Chicken anemia virus (CAV) vaccines. One was vaccinated with 26 P4 strain vaccine subcutaneously and the other was vaccinated with Cux strain vaccine through drinking water (DW). Then the inactivated avian influenza (AI) H5N1 vaccine was inoculated intra-muscularly either at the 1st, 2nd or 3rd WPV with CAV vaccine forming three subgroups per each group. It is clear...
Amany Adel1, Abdelsatar Arafa1, Hussein A. Hussein2 and Ahmed El-Sanousi 2
...ruses were isolated from chickens and ducks in both live bird markets and backyard populations. NS1 gene was amplified, sequenced and the phylogenetic analysis of the obtained sequences revealed deletion of 5 amino acids at positions 80 to 84 in the NS1 protein along all Egyptian isolates under study. Ala149, which is a pathogeneicity marker for interferon antagonism was found in the studied indicating capability of these viruses to transmit between different ...
Serageldeen Sultan1, Nabila Osman2
...) and commercial broiler chickens (n=84) from different farm flocks (24 flocks) in Egypt. These samples were examined for the presence of avian HEV antibody using enzyme linked immuno-sorbent assay (ELISA). The results revealed that the occurrence of avian HEV was 15.7% (80/510) and 66.7% (16/24) of the examined flocks. HEV antibody prevalence increased as age dependent and most commonly occurred among broiler breeder flocks (10/24). In conclusion, the occurre...
Ehab M. El-Nahas1, Hemat S. El-Sayed2, Sawsan S. El-Basuni3, Gabr F. El-Bagoury1
... from 14-day-old broiler chickens with proventriculitis in Qaluobia province of Egypt in 2014. Objective: The isolate was identified by a real time RT-PCR targeting the nucleocapsid (N) gene with further genetic and antigenic characterization. Methods: Beside a real time RT-PCR; sequence analysis of partial S1 gene, cross-neutralization and the challenge experiment for H120 vaccinated broiler chickens were also performed. Re...
Ayman S. El-Habbaa 1, Gabr F. El-Bagoury1, Samar F. El-Adaway2, and Susan S. El-Mahdy2
... occurring in vaccinated chickens with great economic losses. Objective: This work aimed for diagnosis and characterization of Newcastle Disease Virus (NDV) from infected broiler chickens in Egypt. Methods: Suspected virus isolates on embryonated chicken eggs was detected by Hemagglutination (HA) test and identified using hemagglutination inhibition (HI) test. F protein encoding gene of NDV was amplified using Reverse Transc...
Maha M. Khedr1, Reem A. Suliman1, Marwa F. Mohamed1, Mounir D. El Safty1, Hussein A.
Hussein2
...00%, 60% and 60 % of the chickens in tracheal swabs
and 100%, 80% and 60%in cloacal swabs when tested by virus isolation in eggs at 3, 5 and 7 days post
challenge; respectively. Swabs of 10 days post challenge of group 3 were positive by rRT-PCR and
negative by virus isolation. On the other hand, group 6 (vaccinated with imported vaccine) demonstrated
shedding % at 3 and 5 days post challenge by both rt-RT-PCR an...
Shimaa M. Mansour, Reham M. ElBakrey, Ahmed Orabi, Haytham Ali, Amal A. Eid
...rus infection in broiler chickens with
availability of Vertical Transmission
Methods: Herein, 18 chickens derived from 4 broiler flocks within Sharkia Province, Egypt suffering
from different degrees of lameness and/or stunting were clinically and molecularly examined for the
presence of ARV.
Results: By clinical examination, one third (6/18) of examined
Hussein A. Hussein, Maha Khedr, Reem A. Suliman, Marwa F. Mohamed, Mounir D. El
Safty
... and 80% and 20 % of the chickens in tracheal swabs and 80% and 40% in
cloacal swabs when tested by virus isolation in eggs at 3 and 5 days post challenge; respectively. Whereas
swabs of 7 and 10 days post challenge of group 3 were negative by rRT-PCR and virus isolation. On the
other hand, group 6 (vaccinated with imported 500HAU vaccine) demonstrated shedding % at 3 and 5 days
post challenge by rRT-PCR and for ...
Nermeen A. Marden1, Lamiaa M. Gaafar1, Hussein A. Hussein2
...challenge pathway of SPF chickens
to estimate the protection percent of each vaccine against both classical and variant HPAI viruses.
While, the invitro method was done by the direct detection of the viral content and viral identity using
the rRT-PCR technique from final product of these inactivated vaccines.
Results: The data of this study showed that sufficient HA antigen content and similar HA sequences
...
Rana A. Rabiea1, Mohamed Fawzy2, Mohamed H. Khodeir3 and MokhtarM. EL-Tarabili2
...mmune response of turkey chickens.
Methods: Four groups of bronzy turkey chickens were used in this study; group-1 received live
attenuated TRT vaccine, group-2 received inactivated AI vaccine, group-3 received TRT and AI
vaccines and group-4 served as non-vaccinated control. The humoral immune response evaluated
serologically by using hemagglutination inhibition test (HI), ser...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer, Basem M. Ahmed and Ahmed A. El-Sanousi
...the respiratory tract of chickens. In
Lebanon; there is a few data concerning the IBV among poultry flocks.
Objective: The aim of the present study is to detect the molecular characterize IBV in samples
collected from Lebanese flocks geographically distributed in 7 Governorates.
Methods: Blood sera, tracheal swabs and tissue organs were collected from 60 poultry flocks from
different ages, different t...
Riham H. Bassam, Hussein A. Hussein, Mohamed M. Amer and Ahmed A. El-Sanousi
...N2 in different types of chickens including broilers,
breeders, laying hens, backyard chickens and ducks. Tracheal swabs were collected from flocks raised
in different governorates in Lebanon.
Results:Testing of samples with rt-RT-PCR revealed that 6 flocks reared in 4 Lebanese governorates
were positive (CT ranged from 20.42 to 33.86). A Positive sample with CT 20.42 was teste...
Wesley D. Nafarnda, Japhet H. Baraya, Olatunde H. Olabode, Malang K. Simon
...stable ND vaccination in chickens. Sera obtained from forty (40) chickens per LGA during
pre-vaccination, post-vaccination and post-booster vaccination with NDV4 thermostable vaccine were
subjected to Hemagglutination Inhibition (HI) test.
Result: Antibody detection showed seroconversion in chickens 28day post vaccination and post
booster vac...
Karim M. Selim1, Abdullah A. Selim1, abdelsattar Arafa1, Hussein A. Hussein2, Ahmed A. El-Sanousi2
... programs against NDV in chickens,
one using genotype VII inactivated and live NDV vaccine are available in the market and
second group used genotype II inactivated LaSota vaccine with live vaccine.
Method: NDV isolate designated NDV-GZ-986F-2015 was isolated from a commercial
broiler farm in Egypt and characterized as a virulent strain was used for challenge. Contact
chicks were added in both groups ...
Ahmed Ali Moryani1, Nasir Rajput1*, Muhammad Naeem1, Atta Hussain Shah1 and Hidayatullah Soomro2
...e performance of broiler chickens challenged with coccidiosis. A total of 450 broiler chickens were allotted into 6 groups (n = 75/group). Group A controlled without supplementation, Group B, C and D were supplemented with Aloe barbadensis (5ml/L), Ferulafoetida regal (500mg/L), Tamarindus indica (50mg/L), respectively. Group E and F were supplemented with a mixture of all 3 herbs/plants at 2ml/L; mixed with distilled water ...
Guan-bao Ning1, Sheng Niu1, Yue-jian Li1,2, Xiao-xiao Lu1,3, Shi-xiong Yang1, Ali-Raza Jahejo1, Ding Zhang1, Wei-fang Hao4, Wen-wei Gao1, Yu-jun Zhao1, Jian-hui Li1, Fang Yan1, Rong-kun Gao1, Yu-hai Bi1,5, Wen-xia Tian1* and Ling-xia Han6*
...’s disease (MD) in chickens. Chicken erythrocytes are nucleated cells of myeloid origin which contribute to gases exchange, transportation and immunity. The objective of this study was to reveal the immune responses of MDV-infected erythrocytes of chicken by transcriptome analysis. Twenty specific pathogen-free (SPF) chickens were divided into two groups as control and treatment. The chickens
Rizwana Sultan1, Asim Aslam1, Muhammad Yasin Tipu1, Habib ur Rehman2, Saba Usman1, Ahsan Anjum1,*, Muhammad Saeed Imran1, Muhammad Usman3 and Muhammad Zahid Iqbal3
...asitic disease affecting chickens. In Pakistan, there has been no previously published report on phylogenetic analysis of Eimeria tenella. In this retrospective study, tissue samples were collected from a flock of clinically infected chicken followed by haematology, serum biochemistry, and histopathology. Species specific PCR based on polymorphic site of the ITS1 gene was developed and used to identify the organism. Haematological examination of ...
Muhammad Farhan Qadir1, Xin-Yu Han1, Meng-li Qiao1, Qian-qian Cheng1,
Raza Ali Mangi1, Ali Raza Jahejo1, Afrasyab Khan1, Yu-hai Bi1,2 and Wen-xia Tian1*
...ajor cause of illness in chickens with potential threats to human health. Chicken erythrocytes are known as most abundant circulating cells in the body which participate in inflammation and immune responses. Influenza viruses interact with the host cells at cell surface, leading to cell death in human and animals through apoptosis. Current study was designed to investigate the expression levels of apoptosis-related genes in the H9N2 infected chicken erythrocyt...
Jia Liu1, Zhen Wang1, Zifan Ning1, Ali Mujtaba Shah2,3,Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Yaofu Tian1, Diyan Li1,Gang Shu2,4, Lin Ye1 and Xiaoling Zhao1,*
..., and slow- growing (SG) chickens, however, the underlying molecular mechanisms responsible for the differences in post-hatch muscle development are unclear. Here, we report on the identification of several candidate genes that may modulate myofiber growth and thus explain some of the differences in the skeletal muscle phenotype of FG and SG chickens. We collected pectoralis major (PM) and gastrocnemius muscles (GM) on d 1, ...
Ziyue Qin1, Zhen Wang1, Ali Mujtaba Shah3,4, Zifan Ning1, Yaofu Tian1, Qing Zhu1, Yan Wang1, Huadong Yin1, Zhichao Zhang1, Lu Zhang1, Lin Ye1, Diyan Li1, Gang Shu2 and Xiaoling Zhao1,*
...rs. However, whether the chickens with different genetic background have the same response to feed restriction is not reported, herein we evaluated three levels of feed restriction effects on growth performance and blood glucose concentrations in faster- and slower- growing chicken (different genotypes). In the present study, birds were restricted to 100% to full feed in faster- and slower- growing broilers groups. Body weight gain, feed consumption, blood glu...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
... Pradu Hangdum Chiangmai chickens (HPHC). PCR-RFLP protocols were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and ...
Ahmed M. Elbaz*, Engy F. Zaki, Morsy A. S.
... meat quality in broiler chickens. A total of four hundred and eighty 1-day-old Ross 308 chicks were randomly divided into four experimental groups: Control (CON), 5% of quinoa seeds (QS5), 10% of quinoa seeds (QS10), and 15% of quinoa seeds (QS15) groups. Results indicated that increasing inclusion levels of QS improved body weight gain, feed conversion ratio, and crude protein digestibility of broiler chickens. Including q...
Shoaib Ahmed Pirzado1,2*, Wu Zhengke1, Adanan Purba1, Chen Jiang1, Huiyi Cai1, Chen Guilan1 and Guohua Liu1*
...ineralization of broiler chickens. A total of 240-day old male chicks were randomly assigned into comprising four treatments with six replicates (n=10), which included control (basal diet), control +0.25% AZO, control + 0.50% AZO and control + kitasamycin (as AGPs). The results indicate that LBGW, ADG and FCR was significantly (P<0.05) improved in AZO 0.25% and 0.50% than the control. Eviscerated (EV), breast muscle (BM) and leg muscle (LM) were significant...
Keiven Mark B. Ampode1*, Federico C. Mendoza2
...ated immunity of broiler chickens. A total of sixty-day-old Cobb-broiler chickens were used in the study and arranged in a Completely Randomized Design experimental set-up with four dietary treatments. Each treatment was replicated three times, having five birds in every replication. The experimental rations containing graded levels of OP (0%, 1%, 3%, and 5%) were formulated and fed ad libitum in 42 days feeding trial. The r...
Ziyue Qin1, Ali Mujtaba Shah2, 3, Qing Zhu1, Yan Wang1, Diyan Li1, Gang Shu4, Yaofu Tian1 and Xiaoling Zhao1*
...n glucose homeostasis in chickens, we conducted the oral glucose tolerance test, and insulin sensitively test (OGTT and IST) in the fast- (FG) and slow- (SG) growing broilers. Each of 120 one-day-old males was raised in 10 batteries, respectively. In the OGTT test, ten broilers on d 42 fasted 12 h from each stock were randomly assigned into two groups: glucose and vehicle, respectively. The glucose group received a glucose syrup by oral gavage (2 g/kg BW), whi...
Mohamed S. Hussein1*, Hanan A. Fahmy2, Ashraf A. Abd El Tawab3
...parently healthy broiler chickens in Egypt. A total of 150 cloacal swabs collected from broiler chickens from farms and bird markets were subjected to bacteriological and biochemical examination. The results revealed that 92% (138/150) of samples were found positive for E. coli. The antimicrobial susceptibility testing of 72 confirmed E. coli isolates against seven quinolones and fluoroquinolones antibiotics revealed that 62...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Hoda M. Mekky1, Kh. M. Elbayoumi1, 3, Mohamed M. Amer2
... disease particularly in chickens. Thus, this preliminary study was performed to identify intestinal fungal population in immunosuppressed chickens. Cloacal swabs were collected from freshly dead chickens suffering from infectious bursal disease (IBD) for isolation of fungal species on specific medium. A total of 19 purified fungal isolates have been identified morphologically. Aspergillus...
Ho Le Quynh Chau, Than Thi Thanh Tra, Duong Thi Huong, Du Thanh Hang, Le Thi Thu Hang, Vo Thi Minh Tam, Dinh Van Dung*, Ho Trung Thong
...onik (2010) for coloured chickens. At 12 weeks of age, 8 male and 8 female chicks per treatment were randomly chosen for the carcass yield, meat quality, and myogenic gene expression evaluation (myostatin, myf5, MEF2B). The results showed that the higher level of dietary SID methionine than that of Evonik recommendation (2010) improved growth performance in slow-growing colored chickens but had no effect on body weight gain ...
Mohammed H. Galhoum1, Hamza M. Eed2, Essam S. Soliman1*
...| Antimicrobial, Broiler chickens, Conventional, Molecular, Prevalence, Salmonella ...
Mirza Lena1,2, Dinda Fathia Syahramadani2, Alfi Nurrachma Gustya2, Arif Darmawan2,3, Sumiati2, Wiwin Winarsih4, Minoru Maeda5, Komang Gede Wiryawan2*
Mian Abdul Hafeez1*, Adeel Sattar2, Faiza Aslam3, Muhammad Imran3, Kamran Ashraf1, Rashid Zia2 and M. Muntazir Mehdi1
Sajjad Khan*, Naila Chand, Abdul Hafeez, Nazir Ahmad
...enic bacteria in broiler chickens. A total of 160 day-old Ross chicks were assigned to four groups, containing 0, 0.5,1 and 1.5% GA along with basal feed for 42 days. A completely randomized design (CRD) was followed during statistical analysis while difference in means was calculated through Tukey’s test. Results indicated that supplementation of GA at 1.5% significantly (P<0.05) improved feed intake, body weight gain, feed conversion ratio (FCR), Eu...
Alshimaa A. Hassanien1*, Asmaa Osama Tolba2, Asmaa A. A. Hussein3, Walaa M. Elsherif4
...meat products as grilled chickens, grilled beef kofta, and cooked beef meat sold in Assiut city restaurants, and among food handlers working in the same food premises. In addition to the sequencing of 23S rRNA gene and phylogenetic relation between S. aureus isolated from meat products and food handlers. Also, detect the antimicrobial profile and effect of two natural agents of cow milk proteins such as casein and α-lactalbumin on S. aureus isolates of c...
Zulu Blantina Fangele, Tyasi Thobela Louis, Gunya Busisiwe*
...sp;...
Aly M. Ghetas1*, Dalia M. Sedeek1, Hanaa S. Fedawy1, M.A. Bosila1, Asmaa M. Maatouq1, Hoda M. Mekky1, Kh. M. Elbayoumi1,2, Mohamed M. Amer3
...aturally infected native chickens. Thus, bursa specimens were collected from two IBD vaccinated native broiler chicken flocks showing clinical signs, mortality and lesions of IBD in this study. The collected bursae showed pathological changes related to those of IBD infection. Furthermore, the isolates were molecularly characterized using reverse transcription polymerase chain reaction (RT-PCR) technique and gene sequencing of partial portion of VP2 gene. The ...
Shewangizaw Wolde1,2*, Tadele Mirkena1,3, Aberra Melesse1, Tadelle Dessie4, Solomon Abegaz5
...owth performance of male chickens and carcass traits of old hens, and genotype and age effects on carcass traits of male chickens. A total of 150 Normal Feathered local chickens (LL), 150 Sasso-RIR (SRSR) and 150 their F1 crossbred (LSR) chickens were hatched and reared on station until 18-weeks of age. Then, 32 males of each genotype were evaluated for ...
Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3*
...s characteristics of Noi chickens. A total of 183 birds were examined in the study. Body weight was recorded individually at days 1, 35, 63, and 91, and carcass parameters were determined at 91 days for all chickens. For determining single nucleotide polymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A polymorphic site was found in the population, with the frequencies of 11, 12, and 22 geno...
Husmaini1*, Riza Andesca Putra1, Indri Juliyarsi1, Tevina Edwin1, Linda Suhartati1, Aditya Alqamal Alianta1, Harmaini2
...actual population of 610 chickens, an effective population of 600 chickens, and an inbreeding rate of 0.08%. The result showed the farmer’s profile 89.47% of respondents was in 25-55 years old, 47.37% respondent was eduucated in elementary school, 42.11% respondents has 5-10 years farming experience, 56.14% respondents distributed in Jorong Koto Tuo, 98.2% respondend raising KCB as a side business, 56.1% of KCB was for...
Taiwo Oladoye Akande*, Dayo Johnson Ogunyemi, Priscilla Funmilola Okunlola, Emmanuel Owolabi, Odetayo Olakanmi
...the organ weights of the chickens. The fat deposition was substantially reduced (P<0.05) in birds with PFAs. The blood triglycerides, cholesterol, and low-density lipoprotein (LDL) were lower (P<0.05) in groups fed with PFAs. All the PFAs significantly increased the HDL above the value obtained in control. No mortality was recorded throughout the experimentation period. It was concluded that the three PFAs and their mixture used in this study improved th...
Sutopo Sutopo, Dela Ayu Lestari, Edy Kurnianto, Asep Setiaji*
...of the traits. Male Kedu chickens showed more superior than the female ones. EWG showed positive phenotypic correlations to most of BW measured.
Keywords | Average daily gain, Kedu chickens, Mixed model, Phenotypic correlations
...
Jumshaid Iqbal1, Muhammad Sharif1*, Muhammad Nadeem Suleman2, Muhammad Saeed3, Fawwad Ahamd1, Asghar Ali kamboh4, Tugay Ayaşan5 and Muhammad Arslan3
...stomorphology in broiler chickens. One hundred and twenty day-old broiler chicks were randomly distributed into four treatment groups with three replicates per treatment (10 chicks per replicate) under Completely Randomized Design. Four iso-nitrogenous and iso-caloric diets i.e. A, B, C and D were supplemented with a natural growth promoter (NGP) at the rate of 0 (control), 1.5, 3 and 4.5g/kg of feed, respectively. Feed consumption (FC) and weight gain (WG) we...
Muhammad Ammar Dilawar1,2, Hong Seok Mun1, Myeong Gil Jeong1, Eun Ju Yang3, Hyeoung Seog Park4 and Chul Ju Yang1,2,*
Aqsa Fayyaz1, Muhammad Kashif Zahoor1*, Asma Ashraf2, Muhammad Asif Zahoor3, Azhar Rasul2, Humara Naz Majeed4, Muhammad Zulhussnain1, Kanwal Ranian1, Bushra Riaz1, Nazia Khalil1 and Attaullah1
...us of domesticated (i.e. chickens, peacock, pet birds) and wild birds, which is excreted in the fecal material of infected birds. The house fly, Musca domestica L. can gain entry into the poultry flocks and will feed on poultry droppings and potentially serves as a mechanical vector for Newcastle disease. During the current study, houseflies were captured and examined for the presence of NDV. A total of 80 samples were collected from eight different poultry fa...
Thobela Louis Tyasi1*, Lebo Trudy Rashijane1, Kwena Mokoena1, Kagisho Madikadike Molabe1, Madumetja Cyril Mathapo1, Victoria Rankotsan Hlokoe1, Lebelo Joyceline Selala1, Masixole Maswana1 and Lubabalo Bila2
...s a reproduction tool in chickens and a valuable food source for humans. The objective of this study was to examine the effect of egg weight (EW) on egg quality traits such as egg length (EL), egg diameter (ED), yolk weight (YW), albumen weight (AW), shell weight (SW), shell index (SI), yolk ratio (YR), albumen ratio (AR) and shell ratio (SR). Potchefstroom Koekoek layer genotype eggs (n = 200) were used. Pearson correlation and analysis of variance (ANOVA) we...
Md. Taimur Islam1*, Mirza Mienur Meher2, Anas Bin Harun3 and Md. Golam Haider1
...r and in 0-3 months aged chickens. AMPV generally infects the upper respiratory tract of poultry, which has been isolated for the first time in Bangladesh in 2016. Alternatively, adult chickens are more susceptible to IB and ILT. The prevalence of mycoplasmosis and aspergillosis are higher during the winter season and are reported to occur due to poor management systems. Overall, respiratory diseases are most common in the c...
Anis Suraya Mohamad Abir1, Sakinah Yusof1, Abd Rahaman Yasmin1,2, Abdul Rahman Omar1,3, Kok Lian Ho4, Wen Siang Tan1,5, Abdul Razak Mariatulqabtiah1,6*
... after FWPV infection in chickens.
Keywords | Fowlpox, Avian influenza virus, Chicken, Dendritic cells, Haemagglutinin
...
Suhu Duan, Zaiqiang Li, Zhenzhen Fan, Mengran Qin, Xiaoxue Yu* and Liuan Li*
... each group contained 40 chickens that were fed outdoors in an activity area of 63 m2 with free food and portable water. The CK was fed basic diet, and the experimental group added 1%, 3% and 5% linseed oil to the basic diet (TP1, TP2, TP3), respectively. On the 10th, 25th and 40th day of the formal trial, 36 eggs were randomly selected from each group, of which 12 were used to detect egg quality and 24 to detect monounsaturated fatty acids in eggs. The result...
Lopamudra Samantaray*, Yashaswi Nayak
...e performance of Broiler chickens. The study used 280; 70-day-old chicks. The birds were divided into seven experimental groups; one control and six were fed on phytobiotics EOs at rates of 1% and 0.5% and monitored for 8 weeks. The results revealed that the inclusion of 0.5% black pepper and 0.5% fennel combination, as well as a 1% turmeric, significantly (P < 0.05) improved body weights in birds. Black pepper and a combination of turmeric and fennel revea...
Abdelrahman A. Abdelrahman1, Salama A. S. Shany1, Kareem E. Hassan1, Mansy A. A. Dardeer2, Ali A.1*, Magdy F. El-Kady1*
...ral infection in broiler chickens. More field and experimental investigations are crucial to highlight the pathogenesis and the interaction between different respiratory pathogens in chickens.
Keywords | Avian influenza, Broiler chickens, Co-infection, H9N2, Infectious bronchitis Mycoplasma gallisepticum, Mycoplasma synovaie.
...
Mohamed El-Henfnawy*, Essam A. El-Gendy, Ahamed M. El-Kaiaty, Mostafa Helal
Tri Untari1*, Sitarina Widyarini2, Michael Haryadi Wibowo1, Marla Anggita1
...er administration to the chickens. Red ginger powder could provide the highest immunostimulant effect on NDV vaccinated chicken with no toxic effect to the chickens.
Keywords | Antibody response, Immunostimulant, Newcastle disease, Red ginger, Broiler
...
Mohamed Lebdah1, Sahar Abd El Rahman2*, Ahmed Attia3, Reham Karam2, Naglaa Fathy Saeed Awad1, Mohamed Ibrahim El Bagoury1
...ance from NDV strains in chickens and turkeys which is alarming for the potential transmission of NDVs from quails to other poultry species.
Keywords | Newcastle disease, Quails, RT-PCR, F gene, Pathological changes, Class II genotype VII.1.1
...
Hend K. Sorour, Mohammed A. M. Saleh, Azhar G. Shalaby*
Luu Huynh Anh1, Huynh Tan Loc2, Nguyen Hong Xuan3, Le Minh Thanh1, Trinh Thi Hong Mo4, Ly Thi Thu Lan5, Nguyen Trong Ngu1*
... serotype O6 infected in chickens. A total of 420 broilers were randomly assigned to seven treatment groups. The negative control (NC) birds received no E. coli or phages, whereas the positive control (PC) birds were infected with E. coli only. The NC+MHH6 and NC+PR2 treatments received 109 pfu/ml of phages MHH6 or PR2, respectively; whereas the PC+MHH6, PC+PR2, and PC+MHH6PR2 groups were infected with 107.1 cfu/ml of E. coli strain O6 and treated with 109 pfu...
Mohamed. E. Taha1, Mohamed S. Ahmed2, Ahmed I. Ahmed2, Amany Adel3, Salama Rehab4,5*, Nabila Osman2**
...identified among broiler chickens in Luxor governorate in 2016. Five hundred (n= 500) samples were collected from fifty farms (5 tracheal and 5 cloacal pools from each farm) have been examined by real time RT-PCR for the prevalence of AIV H9. The results revealed that ten farms (20%) were positive for AIV (H9N2). Three isolates with high CT values have been selected for complete sequencing of hemagglutinin gene. Amino acid sequences showed that these isolates...
Nishant Shah1*, Rakesh Kumar Yadav1, Anil Gautam1*, Shambhu Shah1 and Mohan Kumar Gupta2
...r the rearing of broiler chickens for better production....
Bulkaini*, Syamsuhaidi, Yusuf Sutaryono, Djoko Kisworo, Sukirno, Sukarne, Tapaul Rozi
...at production of broiler chickens are indicators to assess carcass quality. This study was conducted to evaluate the carcass characteristics and production of pure meat broiler chicken circulating in the traditional markets of Lombok and Sumbawa based on the Indonesian National Standard. The material used was 191 heads of broiler chickens with a slaughter age of 45-49 days. Ninety heads from a chicken slaughterhouse in Matar...
Muhammad Kashif Iqbal1*, Khalid Mehmood2,4, Shakeel Ahmed3, Fazul Nabi4, Muhammad Arif Rizwan1, Muhammad Kaleem1, Mushtaq Ahmed1, Abdul Waheed1 and Jiakui Li5
... growth plate in broiler chickens. So, it is effectual through rectifying the oxidative imbalance and liver damage....
Ahmed A. Mohammed1, Madeha H. A. Darwish1, Eman A.A. Negm2, Ayman S. Abdel- Maguid3
Effects of Antibiotics on Growth Performance, Immune Response, and Intestinal Microflora of Broilers
Shahan Azeem1*, Muhammad Akram Muneer2, Liaquat Ahmad3, Sameera Akhtar1 and Talat Naseer Pasha4,5
...rty-five day-old broiler chickens were randomly divided into five treatments and housed under identical husbandry conditions. Antibiotic-free poultry feed was procured from a commercial feed mill. The antibiotics were mixed in the feed as per the manufacturer’s instructions and offered ad libitum to the chickens for the entire study period. The chickens were vaccinated for NDV and AI...
Zahra Naz1,2, Fouzia Ismat1, Muhammad Saleem2, Mazhar Iqbal1, Aamir Shehzad1 and Moazur Rahman1,2*
...ewcastle disease (ND) in chickens. Owing to its highly conserved nature among NDV strains and also due to its pivotal role in the viral life cycle, the M protein can be employed as a promising diagnostic antigen for reliable detection of NDV infection in chickens. In the present study, we have devised a strategy for extraction and solubilization of the NDV M protein from Escherichia coli in a single step using a non-ionic de...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...ation. Total 120 broiler chickens were randomly distributed in ten groups. The groups included negative and positive control, pure organic acid group and commercial organic acid formulation group. Excluding negative control group, all other groups were orally challenged with 0.1 ml of the 6-Log 10 CFU/ml of the Campylobacter jejuni culture in normal saline via oral route. Cloacal samples were collected for Campylobacter count, body weight (BW) and feed convers...
Muhammad Halim Natsir*, Osfar Sjofjan, Yuli Frita Nuningtyas, Danung Nur Adli, Lizar Basnelda, Dimas Frisky Apriyanto, Wahyu Tri Utami
...ial population of Lohman chickens. A total 256 Lohmann chicken were used on the experimental diets. The Fountain clerodendrum and avocado seed flour were enhanced with nanotechnology 500 mm. The two-way nested Anova with eight treatments and four replication was employed, with each experimental unit consisting of eight Lohmann broiler chickens. The treatments consisted of B1T0 = basal diet, B1T1 = basal diet + 0.4% avocado s...
Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1*
...controlling infection in chickens.
Keywords | Curcumin -olive oil nanocomposite, anticoccidial activity, Comet assay, Poultry, coccidiosis.
...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed Elbayoumi1 , Sameh Abdel- Moez Ahmed Amer1, Asmaa Mahmoud Matoaq1 , Mohamed Abdel Aziz Kutkat1, Gomaa Abd El-Rhim Abdel-Alim2
...o 260 commercial broiler chickens in 13 groups; 20 birds each. At 28 days-old, all groups were challenged with NDV genotype VII 1.1 strain “NDV-CHICKEN-EGY-ALEX-NRC-2020” to evaluate the protective immunity of applied protocols. Humoral immune response, clinical signs, post-mortem gross lesions, growth performance as well as mortalities are all recorded and evaluated. All vaccination protocols were able to induce antibody levels for NDV after vacci...
Mai A Fadel1*, Ahmed M Elmahdy1, Jihan Mostafa Badr2, Mohammed AM Saleh3, Mona A.A. AbdelRahman3
...xed infection in broiler chickens. One-hundred and five chickens were equally divided into 7 groups. Group one was used as a non-treated non-infected group (negative control). Group two was used as an infected with Escherichia coli and Salmonella enteritidis (mixed infection), non-treated group (positive control). Group three was infected with the mixed infection and treated with amoxicillin 13.1 mg/kg b.wt. Group four was i...
Sekobane Daniel Kolobe, Tlou Grace Manyelo*, Jones Wilfred Ng’ambi, Munyadziwa Felicia Dorcus Nemauluma, Emmanuel Malematja
...t morphology of Ross 308 chickens from day-old to 6 weeks of age. 320-day-old Ross 308 broiler chicks were assigned to a 2 (sex) × 4 (dietary treatment levels) factorial arrangement in a CRD, having 8 treatments, replicated x4 with 10 chicks/replicate. AKLM inclusion levels were at 0, 0.5, 1.0 or 1.5g/kg dry matter (DM). Weekly feed intake (FI), live weight (LW), and growth rate (GR) were measured to obtain the feed conversion ratio (FCR). Gut character...
Ahmed H. Abed1, Ahmed Orabi2*, Wafaa Hassan3, , Enas Hammad4 , Ghada Gaber5
...iating effect on broiler chickens’ immunity challenged with E. coli. One hundred and twenty broiler chicks were separated into eight equal groups in an in vitro experiment (15 birds for each group). A negative control group was the first group. There were 3 groupings were given immune modulatory agents (lectins, probiotic, and essential oil) for five days without being challenged; Immune modulatory agents were given to the remaining three groups, who wer...
Tlou Grace Manyelo1,2, Nthabiseng Amenda Sebola1, Jones Wilfred Ng’ambi2, Monnye Mabelebele1*
... of indigenous Boschveld chickens. A total of 200 one-day-old indigenous Boschveld chicks were randomly allocated to five treatments in a completely randomized design each with four replicates of ten chicks. Amaranth leaf meal (ALM) levels evaluated in this study were 0, 5, 10, 15, and 20% with recording weekly body weights, feed intakes, and feed conversion ratios. Gut organ weights, lengths, pH, and meat characteristics were determined, and the general linea...
Hemat A. Abdel Magied*, Heba H. Habib, Amany H. Waly, Ahmed Fadl, Sayed M. Shalash
...y of Arbor Acres broiler chickens. Two experiments were conducted in current study; experiments 1 (EXP1) evaluated the effects of PE and/or P supplementation to standard corn-soybean broiler diet (STD). while experiment 2 (EXP2) examined the PEP supplemented to different levels of low CP (low 10 or 15 %) and low ME diets (lower 100 or 150 kcal/kg) than control (L10%-100, L10%-150, L15%-100, L15%-150). The obtained results were: the EXP1 showed that significant...
Andy1,4*, Ratmawati Malaka2, Sri Purwanti3, Hikmah M Ali2, Tutik Lusyta Aulyani4, Danung Nur Adli5
...obial profile of broiler chickens. A total 100 broiler chickens were reared for five weeks on the experimental diets. A general linear models with five treatments and four replications was employed. The treatments consisted of T0 = control, T1 = basal diet + acidal, T2 = basal diet + 0.5% encapsulation of liquid smoke, T3 = basal diet + 1.0% encapsulation of liquid smoke, T4 = basal diet + 1.5% encapsulation of liquid smoke....
Sugiharto Sugiharto*, Ikania Agusetyaningsih, Endang Widiastuti, Hanny Indrat Wahyuni, Turrini Yudiarti, Tri Agus Sartono
...n CONT, HSD-P and HSD-PC chickens consumed more (P < 0.05) feed than HSD chickens. Body weight and weight gain were lower (P < 0.05) in HSD compared to the CONT, HSD-P and HSD-PC groups. The European Production Higher Efficiency Factor (EPEF) tended (P = 0.06) to be higher in HSD-PC than in HSD chicks. While there was no significant effect of the treatments on complete blood counts and serum biochemical parameters, ser...
Istna Mangisah1*, Heni Rizqiati2, Nyoman Suthama1
...starter. Day-old broiler chickens with an average body weight of 45.61 ± 3.46 g, a total of 160 birds, were placed evenly in 20 experimental plots. The treatment tested was the LDTE level in 100 grams of feed, LDTE0 = without LDTE, LDTE1 = 1 mL, LDTE2 = 2 mL, and LDTE3 = 3 mL. The treatment was given for 21 days, starting from d-1 until d-21. Intestinal microbial population, weights of digestive and immune organs, nutrient digestibility, and growth per...
Muhammad Bilal Munawar1, Saima Naveed1, Shafqat Nawaz Qaisrani1, Jibran Hussain2, Shahzad Munir3, Ayesha Ahmed3, Mubbashir Hussain4, Ahmad Kamran Khan5, Abdullah Jalal6 and Irfan Ahmed7*
... (n=504) day-old broiler chickens procured randomly allocated into 6 treatments dietary treatments were: B positive control (PC) diet (0.42% available phosphorus in grower feed, A negative control (NC) diet with (0.30% avail. P in starter and 0.26% avail. phosphorus in grower) while four diets C, D supplemented with NC+ (500 and 750 powder phytase units (FTU)/kg) and G, H supplemented with NC+ (500 and 750 liquid phytase units (FTU)/kg) respectively. Pelleting...
Ahmed Rizk1, Gomaa Said Ramadan2*, Alaa Eldin Abdou3, Ossama Ahmed El-Weshahy1, Omer Sayed Rashed1
... Tanta G- 1 and Mamourah chickens strain respectively. In the first generation age at sexual maturity for Tanta G- 1 was 172 days, while it was 157 days for the Mamourah strain. The weight of the first egg weight was (45.46g) for Tanta G- 1, while it was 34.84g for the Mamourah strain (34.84g). The overall mean fertility percentage for Tanta G-1 female egg was 87.92 %, while it was 88.94 % for the Mamourah strain and the difference was significantly differen...
Reda Hassan*, Bahaa Abou-shehema, Sherif Zayed, Micheal Gorgy, Shama Morsy, El-Sayed Abu El-Hassan, Mahmoud El-Gbaly, Hanaa Basuony, Ebtehal Hassan
...cks). On the other hand, chickens were offered the basal dietas Negative Control (NC, group 1); the other three groups 2, 3, and 4 were fed NC supplemented with 0.5% HSCAS, SC and HSCAS plus SC respectively, group 5 chicks were fed a basal diet contaminated with 1ppm AFB1 as Positive Control (PC), the other three groups 6, 7 and 8 were fed PC supplemented with 0.5% HSCAS, SC, and HSCAS plus SC respectively. Results exhibited that broiler chicks fed with a PC d...
Pham Tan Nha*, Le Thu Thuy
...the performance of local chickens in the south of Vietnam. It was (3*2*3 factorial) with 2 factors. Factor fermentation: Yeast-fermented maize (YM), Yeast-fermented broken rice (YR), Probiotic fermented maize (PM). Factor protein level: 20% (CP20) and 16% (CP16). One hundred and eighty Noi chickens at 6 weeks of age (334±9.5 g/bird), and 10 birds per unit (balanced sex) were in the treatment. All treatments were added...
Andi Tenri Bau Astuti Mahmud1, Lellah Rahim2, Muhammad Ihsan Andi Dagong2*, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2
...ual feed intake (RFI) in chickens, which is usually calculated during the growth period, is a measure of feed efficiency that does not depend on the level of production. The study aimed to evaluate the growth performance, carcass quality, physical meat characteristics, and sarcomere properties of Kalosi chicken, which were grouped according to high and low Residual Feed Intake (RFI) phenotype. This study used 64Kalosi chickens
M. Mansoor Javed*, M. Aslam Mirza, M. Aziz ur Rehman and M. Saif ur Rahman
...on and growth in broiler chickens. Full-fat soybean was prepared by subjecting dehulled soybean to different temperatures i.e. 90°C, 100°C and 110°C for various resident tines i.e., 15 min, 30 min and 45 min in a multitier commercial toaster. Each product was analyzed for proximate composition, pepsin digestibility, KOH solubility and urease activity prior to inclusion in the experimental diets. A day old Cobb-500 broiler birds (n=432) were purchas...
Sri Setyaningrum*, Dini Julia Sari Siregar, Tengku Gilang Pradana
...ut microflora of broiler chickens. The research used 144 one-day-old broiler chicks (44.33±1.09 g). The research was conducted with a completely randomized design. The treatments included of T0: diet + 0% CGLp (control), T1: diet + CGLp 1.0%, T2: diet + CGLp 1.5%, T3: diet + CGLp 2.0%, T4: diet + CGLp 2.5% and T5: diet + CGLp 3.0%. The treatment of CGLp was significantly increased (p <0.05) of body weight gain, live body weight, carcass percentage, m...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel4, Abdelwahab M. Abdelwahab1,5
...nged to the W-36 Hy-Line chickens. The layers were randomly designated into five equal treatment groups (9 replicates × 6 hens per replicate for each treatment) according to the dietary inclusion levels of BSFL meal. The first group of birds were fed a basal diet of soybean-corn meals and served as a control (0% BSFL meal), while the remaining 4 groups were fed a basal diet in which the soybean-corn meals were partially replaced with 3%, 6%, 9%, and 12% ...
Farid S. Nassar1,2, Abdulaziz M. Alsahlawi3, Mohammad A. Al-Mahaish4, Ahmed O. Abbas1,2*, Abdulaziz A. Alaqil1, Nancy N. Kamel5
...ic efficiency of broiler chickens. The study included 360 unsexed Cobb500™ commercial broiler chicks aged 1 day and weighing 46±1.5 g. Starting from the 22nd to the 42nd d of age, the chicks were randomly distributed according to the dietary inclusion levels of MWM into six treatment groups (6 replicate pens per group × 10 chicks per replicate, considering the equal ratio of males to females in each replicate). The first group of birds was f...
Hagar Magdy Ahmed1, Mohamed Mahrous Amer2*, Khaled Mohamed El-Bayoumi1, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila1, Gomaa Abd El-Rhim Abdel Alim2
...;) in commercial broiler chickens. The pathogenicity studies parameters included the effects on protection, growth performance and clinico-pathological changes. The results indicated that, immunostimulants can keep maternal immunity longer where lector® was the best; the decline in HI titers also indicates that bird groups not contract ND natural infection. The results of performance parameters after immunostimulants administration and challenged with IBDV...
Fatma Salih Mohammed1*, Nasser Khedr1, Tahia Ahmed1, Liza S. Mohammed2
...ne-day-old Ross® 308 chickens were randomly distributed into three groups with different dietary treatments (66 birds per group). The dietary treatments were as follows: D1, control (soybean meal 46%); D2, D1 + EE-SBM (50 kg starter, 100 kg grower, and 200 kg finisher/ton); and D3, D1 + EE-SBM (100 kg starter, 150 kg grower, and 250 kg finisher/ton). The study results showed that the addition of EE-SBM to broiler diets had no significant effect on carcass ...
Yusri Sapsuha1*, Nur Sjafani1, Suryati Tjokrodiningrat2
...e performance of broiler chickens is focused on maximizing performance parameters, i.e., fast chicken growth with low feed consumption, and with optimal health status. This study provides an in-depth review of the use of cloves in feed to improve broiler chicken production performance and health. In conclusion, cloves contain bioactive substances that can be used to improve broiler chicken performance and health. Administration of appropriate dose is believed ...
Abdul Azis*, Afriani Afriani
...lications. As a control, chickens were fed ad libitum (FR-0); chickens were given free access to feed during two periods of 2 hours from age 7-14 days (FR-1) and age 7-21 days (FR-2); chickens were given free access to feed during two periods of 4 hours from age 7-14 days (FR-3) and age 7-21 days (FR-4). Body weight, carcass yield, breast, and tight muscle weight of broilers subjected...
Ahmed Ali Chandio1, Asghar Ali Kamboh1*, Nazar Ali Korejo2, Riaz Ahmed Leghari3 and Muhammad Ali Chandio4
Riko Noviadi, Dwi Desmiyeni Putri, Gusma Gama Maradon*, Agung Adi Candra, Nani Irwani, Gadis Apriani, Made Guntur Candra Adinata, I Made Krisnanda
Doungnapa Promket1,2,4*, Khanitta Pengmeesri2,4, Jennarong Kammongkun3, Thassawan Somchan2
...raits in 300 Thai native chickens were investigated. DNA was extracted from blood samples for genotyping using specific primers and restriction enzymes for each gene, and polymerase chain reaction-restriction fragment length polymorphism was used to identify the genotypes (PCR-RFLP). Three genotypes were found for each gene as BB, Bb and bb for NPY; TT, TC and CC for DRD2 and II, ID and DD for VIP. Genotype frequencies of NPY (range 0.13-0.58), DRD2 (range 0.0...
Zulaikha Zainool Abidin1, Fahim Hafiy Idris2, Suriya Kumari Ramiah1, Elmutaz Atta Awad1, Zunita Zakaria3,4, Zulkifli Idrus1,2*
...bacillus spp. in broiler chickens. Thirty-five-day-old broiler chickens were subjected to either 0 h or 8 h of feed withdrawal, followed by 2 h or 4 h of road transportation. For each feed withdrawal-transportation subgroup, the birds were lairaged for 0 h or 3 h. Salmonella spp. and Campylobacter spp. were only detected in two and three birds, respectively. Because of the insufficient data, both types of bacteria were exclu...
Hendy Musbawandi1, Mohammad H. Tamzil2*, Budi Indarsih2
...domized design using 180 chickens. All chickens were divided into three groups with six replicates each which were the first group was reared in a closed house, the second and third groups were reared in positive pressure and open houses. Each replicate, in each treatment, was reared in a different barn plot with the number of 10 chickens/m2 of the barn area. The results showed that feed c...
Tanvir Ahmed1, Sachchidananda Das Chowdhury1, Bibek Chandra Roy1Shubash Chandra Das1*, Khan Md. Shaiful Islam2, Bipul Chandra Ray1, Sukumar Saha3
...mune response in broiler chickens. A total of 450 Cobb-500 unsexed DOCs were divided in response to the treatment into broilers fed a control diet (no additives), control supplemented with PB (100 g/100 kg feed), or control supplemented with AGP (Lincomycin 2.2%, 15 g/100 kg feed), having six replications in each treatment (25 birds per replication). Growth parameters were recorded. On days 22 and 34, HI antibody titer was determined against Newcastle disease ...
Muhammad Arshad Iqbal*, Aamir Ghafoor, Irfan Ahmad, Muhammad Ijaz
...f 180 commercial broiler chickens were obtained from local hatchery and were reared for 42 days. Three groups (A, B, C) of birds were divided into 3 subgroups with 20 birds in each group. Group A (-ve control) received diet without AGP or probiotic. Group B (+ve control) was fed with AGP. Group C was probiotic feed supplemented with B. amyloliquefaciens. All subgroups 1 were kept non-challenged, at day 21, subgroups 2 were challenged with 1x105 CFU of S. Enter...
Maria Endo Mahata1*, Oriyanti Br Situngkir1, Yan Heryandi2, Takayuki Ohnuma3, Yose Rizal1
...dy used 30 broiler male chickens with an average body weight of 1.5 kg at five weeks of age. This study used an experimental method with a completely randomized design (CRD) of a 3x3 factorial pattern which was repeated three times. Factor A (type of pellet binder): A1 (brown seaweed), A2 (taro tuber), and A3 (tapioca flour), then factor B (dosage of pellet binder): B1 (1.5; 3.3; and 4.5%). Crude fiber digestibility, nitrogen retention, and energy metabolism ...
Abdulaziz A. Alaqil
...on. Four hundred broiler chickens (Cobb500, 1-d-old) were randomly distributed in equal numbers in 40 battery cages (10 birds per cage). Every ten cages were placed in a room to arrange four experimental room groups identified as C, EC, PR, and PR+EC groups, respectively. From 22-42 d of age, the C and EC groups have received a basal diet with no PR supplements, whereas the PR and PR+EC groups have received PR at 1 g/kg of the basal diet. On day 36 of age, the...
Md Tariqul Islam1,5 *, Aditya Chowdhury Avi1,5, Md Saiful Bari2,5, Most Rebeka Sultana Swapna Khandoker3, Homaira Pervin Heema4, Goutam Buddha Das1
...aracteristics of broiler chickens. In a 28-day study, Investigation was carried out using 120 Ross-308 unsexed day-old chicks (DOC) of broiler chicken. Birds were selected by random selection to assign them to each of four feed trial groups: T0, T1, T2, and T3, and each of those received 0, 20, 30, and 40% PM supplementation. The result showed that no significant variations were observed in survival rate, feed conversion ratio, or carcass yield within the broi...
Nguyen Thi Anh Thu1*, Ly Thi Thu Lan1, Nguyen Thuy Linh1, Chau Cong Dang1, Nguyen Hoang Phuc1, Tran Dinh Tuan1, Nguyen Trong Ngu2
...s (SE) in native broiler chickens. Totally, 320 native chicks were divided into two experiments using a completely randomized design consisting of 4 treatments and 4 replicates of 10 chicks per experiment. In both experiments, BC was used at a dose of 109 PFU/mL, and SE was used at 108,2 CFU/mL. In the protective experiment, 1-day-old chicks were arranged in CD) control diet; BC+S) CD + 1 mL BC (gavaged at days 1, 2, 3, 4, 5) + 1 mL SE (gavaged at day 3); S) C...
Fuji Astuty Auza1*, Sri Purwanti2, Jasmal A. Syamsu2, Asmuddin Natsir2, Rusli Badaruddin1, Deki Zulkarnain1, La Ode Muh. Munadi1
... the proper nutrition of chickens, but their availability is often limited, and their cost is typically high. Therefore, this study aims to develop a ration formulation using black soldier fly (BSF) larvae flour to reduce the use of fish flour and improve performance, carcass quality, and nutrient digestibility of village chicken. A total of 140 one-day-old chicks (DOC) were randomly assigned to one of five treatments in a completely randomized design, with fo...
Rizki Palupi*, Arfan Abrar, Fitri Nova Liya Lubis, Yuni Kurniati, Bianca Ikriza Octa Nadia Putri
...vels in blood of broiler chickens. Data were analyzed by ANOVA and further tested by Duncan Multiple Range Test (DMRT). The results of this study indicated that the CCI had a significant (P<0.05) effect on nutrient digestibility, hematological status and malondialdehyde levels in blood of broiler chicken. The CCI at a level of 10% in the ration increased hemoglobin levels by 17.09% and reduces malondialdehyde levels by 14.28% in broiler blood compared to co...
Abiyu Tadele 1,2*, Gebreyohannes Berhane2, Wondmeneh Esatu3
...ong the genetic group of chickens, demonstrating that choosing birds with bigger eggs increases the proportion of edible eggs. As a result, our findings will help to develop more effective breeding programs for these three chicken genotypes by providing valuable insights into the genetic basis of chicken performance and egg quality. ...
Yoshio Hamano1*, Yasuji Kurimoto2
...tabolism. ...
Xiangjie Zhao1, Zheshi Kuang2, Jiyuan Yang2, Muhammad Bilal1 and Rongling Yang1*
...f Lingnan yellow broiler chickens. Lingnan yellow broilers were randomly categorized into 4 groups. The control group was fed with basal diet containing 3% fishmeal, and the treatment group 1, 2, and 3 were fed basal diet with 30%, 60%, and 100% fishmeal substituted with fermented silkworm pupae (FSP), respectively. Broilers were fed the treatment diets in phase 1 (1-21 d) and phase 2 (22-42 d) during the experimental period. Results showed a non-significant d...
Ertika Fitri Lisnanti1,4, Widya Paramita Lokapirnasari2*, Eka Pramyrtha Hestianah3, Mohammad Anam Al Arif2, Zein Ahmad Baihaqi4,5
...axillary vein of broiler chickens which is put in an EDTA tube. The parameters measured were: the number of leukocytes, erythrocytes, hemoglobin levels, hematocrit, platelets, lymphocytes, monocytes, granulocytes, types of leukocytes and blood glucose levels in broilers. The addition of different of Marsh fleabane leaves water extract in the feed had a significant effect on the levels of Hb, leukocytes and erythrocytes were within the normal range while all bl...
Pham Tan Nha*, Le Thu Thuy
...e study involved 180 Tre chickens, aged 6 weeks and weighing approximately 200±6.5 g/bird. Each experimental unit consisted of 10 birds, with a balanced distribution of sexes. The treatments included FG0.5, FG1.0, FG1.5, BG0.5, BG1.0, and BG1.5, where Fresh Garlic and Black Garlic were added to the basal diet at 0.5%, 1%, and 1.5% (DM) concentrations. The trial lasted for 8 weeks, covering the period from 6 to 13 weeks of age for the Tre
Mohamed Mahrous Amer1*, Hanaa Sayed Fedawy2, Hoda Mohamed Mekky2, Khaled Mohamed Elbayoumi2, Ahmed Ali El-Shemy3, Mohamed Abd El-Rahman Bosila2
...day old broiler Ross 308 chickens subcutaneous (s.c) injected with E. coli and/ or S. aureus to induce cellulitis. Clinical signs, mortality, pathological lesion, and growth performance were determined. Hematological parameters, liver and kidney functions were also recorded. Colistin+ Doxycycline combination (Doxyforte®) was used to control the infection. Clinically, site of infection was appeared red, swollen accompanied with increased skin thickness, pos...
Imbang Dwi Rahayu1, Ali Mahmud1*, Wahyu Widodo1, Adi Sutanto1, Apriliana Devi Anggraini1, Devi Dwi Siskawardani2, Wisnu Nurcahyo3, Tri Untari3
...lood. In this study, 175 chickens were used and divided into seven levels of treatments; T0 (feed without herbs), T1 (feed + 1% herbs), T2 (Feed + 2% herbs), T3 (Feed + 3% herbs), T4 (drinking water +1% herbal), T5 (drinking water + 2% herbal), T6 (drinking water + 3% herbal). The data were analyzed using a one-way analysis of variance. As a result, the treatments did not affect (p>0.05) the total plasma protein (TPP), globulin, cholesterol, high-density li...
Sameh Abdel-Moez Ahmed Amer1*, Aly Mohammed Ghetas1, Asmaa Mahmoud Maatouq1, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1, Mohamed Abd El-Rahman Bosila1, Ahmed Ali El-Shemy2
...e II vaccines in broiler chickens. Eighty chickens were housed in four groups (A, B, C and D) of 20 birds per group. Group A has been received the experimentally prepared inactivated genotype VII NDV vaccine by day 9 old, subsequently primed and boostered with commercial live attenuated genotype VII vaccine at 7 and 21 days-of age. While group B was treated with commercial live and killed genotype II NDV vaccines at the same...
Mai M. Dawood*1, Mohamed Fawzy2 and Mohamed S. Elshahidy3
...tagious disease of young chickens, that results in bursal necrosis and severe damage of the immune system. IBDV remarkably persist to affect the poultry industry in Egypt. Hence this study was done to identify and characterize very virulent Infectious Bursal Disease virus in Egypt during the period 2019-2020. bursal samples were collected from 8 vaccinated broiler flocks exhibited high mortality rates and bursal lesions in al-El-Fayoum province, Egypt during 2...
Mahmood Davoodi-Omam, Hossein Mansoori Yarahmadi*, Jafar Fakhraei and Mehdi Changizi
...ts, 6 replicates with 10 chickens in each replicate up to 42 days of age. The group containing Globacid (1 mL/L) and the restriction intensity of 10% had the highest significant difference compared to the control (p <0.05). The highest thymus weight was observed in the treatments with Globacid levels of 0.5 and 1 mL/L, respectively (p < 0.05). Also, the highest humoral immune response among the groups was seen in the group containing Globacid (1 mL/L) an...
Zeyad Kamal Imari
...sixty commercial broiler chickens were used in this study. The broiler chickens were randomly placed in 36 pens with 6 groups, each group contained 6 pens with 10 birds for each pen. Six experimental diets with different levels of calcium (Ca) were formulated to be as follows: 1.4% Ca, 1.2% Ca, 1% Ca, 0.8% Ca, 0.6% Ca and 0.4% Ca. The polynomial regression equations were used to estimate nutritional requirements of calcium....
Olabode, H.O.K1, Mailafia, S1, Martha Echioda-Ogbole1*, Ameh, J.A1, Amadi, K.I1 and Meseko C.A2
...ence of AI antibodies in chickens and AI appraisal of poultry farmers’ knowledge, attitude and practice in Abuja Municipal Area Council, Nigeria. One hundred (100) blood samples collected randomly post slaughter from chickens in live bird markets were screened and characterized using Agar Gel Immuno Diffusion and Haemagglutination Inhibition Tests at the Avian Influenza laboratory, of the National Veterinary Research I...
Vijay Kumar1, Asghar Ali Kamboh1*, Nazar Ali Korejo1, Ambreen Leghari2, Faiz Muhammad Khand2, Riaz Ahmed Leghari1, Khaliq-ur-Rehman Bhutto3
...ganisms is quite high in chickens (broilers and layers) as compared to ducks. Moreover, gentamicin, followed by ciprofloxacin, norfloxacin and enrofloxacin could be the drug of choice against Salmonella of poultry origin. ...
Shafqat Ullah1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Raheela Taj3, Fatima Syed3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Muneeb Islam7 and Sumaira8
... and on the last day two chickens from each replicate were slaughtered (a cumulatively eight chickens from each group). Samples from different regions (duodenum, jejunum, and ileum) of the small intestine were collected and kept in the fixatives. Slices from each part were stained with H and E stain to make it fit for microscopy. Picture were taken with different magnifications, i.e., 4X, 10X and 40X and histo-morphometry wa...
Raed Hussein Salih Rabee1, Yahya Sabah Abdulameer1, Walla Farhan Obed1, Noor R Abady1, Adnan Mansour Jasim1*, Firas Hussein Albawi1, Mohammed Jasim Jawad2, Ahmed Samir Abukhomra3
... coli) was isolated from chickens suffering from diarrhea, and its isolation was confirmed by culturing it on MacConkey agar medium. In this study, a total of one hundred chicken chicks were reared, and on the tenth day of the experiment, they were divided into five equal groups:NCG: This group represented the control and was given normal drinking water. T1: The chickens in this group were orally inoculated with E. coli at a...
Rusli Tonda1, Lili Zalizar1*, Wahyu Widodo1, Roy Hendroko Setyobudi1, David Hermawan1, Damat Damat1, Zane Vincēviča-Gaile2, Irum Iqrar3, Trias Agung Pakarti4,5, Shazma Anwar6 and Wirawan Wira7
... rice bran (T3). Broiler chickens aged 35 d with an average body weight of 1 870 g were involved – each treatment unit was of three, making a total of 27 chickens. Data gained from the experiment were run through one-way ANOVA, followed by an LSD test should any differences in the treatments occur. The result showed that consumption on T2 (59.89 g) was higher than on T1 (42.19 g) and T3 (9.22 g). As of feeding duration...
Afshan1, Asad Ullah2*, Imad Khan2, Rafiq Ullah1, Tahira Tayyab1, Fatima Syed3, Raheela Taj3, Shumaila Gul4, Faiza Khan5, Ibad Ullah Jan6, Syed Weqas Ali7 and Assad Ullah8
...) in each group. Broiler chickens feed with starter and finishing commercial corn based basal diet with different concentration of selenium powder. Basal diet (BD) was fed to Group A, which kept as negative control group and no dexamethasone (DE) was offered. Group B was considered as (+ve control group) fed with (BD+15mg DE/kg). The group C, was fed with (BD+0.2mg Se + 15mg DE/kg). Similarly, group D was fed with (BD+0.3mg Se+15mg DE). Group E, fed with (BD+0...
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...anges. Sixty (60) sonali chickens of twenty-three days old were randomly divided into five groups and each group contained 12 chickens. All groups were supplied E. tenella orally except the T0 group and after 3 days T2 and T3 group was treated with yeast 1g/kg and 1.5g/kg feed for 14 days and T4 groups were treated with amprium-vet Powder (amprolium hydrochloride 20%) at a dose rate of 1g per 3 liters of drinking water for 7...
Md. Ariful Alam1, Mst. Misrat Masuma Parvez1, Md. Salauddin2*, Rakibul Islam1 and Md. Bazlar Rashid1
...anges. Sixty (60) sonali chickens of twenty-three days old were randomly divided into five groups and each group contained 12 chickens. All groups were supplied E. tenella orally except the T0 group and after 3 days T2 and T3 group was treated with yeast 1g/kg and 1.5g/kg feed for 14 days and T4 groups were treated with amprium-vet Powder (amprolium hydrochloride 20%) at a dose rate of 1g per 3 liters of drinking water for 7...
Khanitta Pengmeesri1,2* Thassawan Somchan1 , Doungnapa Promket1,3
...tage. ...
Franciscus Rudi Prasetyo Hantoro1,2,*, Dwi Sunarti1, Turrini Yudiarti1, Sri Sumarsih1, Rini Nurhayati2
...ection (SenSi) 1 Agrinak chickens. Treatments consisted of three stocking densities (10, 14, and 18 birds/m2) and three levels of crude protein (14, 16, and 18%) factorially (3×3) which were arranged in nine treatments and four replications. Treatment and data collection were carried out at the age of 29-70 days. Data were analyzed using ANOVA with a factorial Group Random Design. The results showed that stocking density and crude protein levels had no i...
Ho Thi Dung, Nguyen Van Chao, Nguyen Thi Hoa, Le Dinh Phung, Tran Thi Na, Nguyen Thi Thuy, Tran Nguyen Thao, Pham Hoang Son Hung*
...at stress. Fifty broiler chickens at 14 days old were randomly allocated to the thermoneutral control (TC) treatment and the high ambient temperature (HT) treatment (5 cages of 5 chickens per treatment). The broilers of the TC treatment remained under a constant 26 ± 1°C (mean ± standard deviation), while those of the HT treatment were maintained under a constant high temperature of 35 ± 1°C. At ...
Turrini Yudiarti*, Sugiharto Sugiharto, Endang Widiastuti, Hanny Indrat Wahyuni, Tri Agus Sartono, Maulana Hamonangan Nasution
...data were collected from chickens that were 42 days old. The results shown that the dose of feed additive up to 1.0% increase cholesterol value, total protein, MCHC, Coliform population in ileum and weight of heart also bursa of fabricius, whereas could reduce MCV, coliform population in caecum, and weight of pancreatic also caecum and no effect on broiler performance. The conclusion is supplementation of fermented product of the fungus Monascus purpureus as a...
Madboulyl , H. M.; Tamami , S.M.; Abd Elmoneml , A.S.; Husseinl , A.S. and Arafa2, A.M.
...re obtained from broiler chickens from El-Fayoum, Beni suef and Giza, 40 turkey poults serum samples from Al Behira and El Monofia, 278 chicken egg yolk samples from El-Monofia, Dommiat, Alexandria and Al Behira and 675 turkey egg yolk samples from El-Fayoum and Beni suef Governorates) were tested by using ELISA. The percentages of positive reactors of chicken sera and egg yolk samples were 57.4% and 74.4%, while it were 77.5% and 83.2% respectively in turkey ...
Hussein, HA; sultan, HA. Al Deeb AH and AA. El-Sanousi
... outbreaks in vaccinaied chickens housed in poultry farms in Sharkia governorate in Egypt was observed starting from October 2007 and continued for 3 months until control measures have been taken in such farms. besides multiple vaccination during this period were applied using Reassortant H5N1 and 6 types Of H5N2 vaccines. In the present study, 9922 serum samples were collected from vaccinated chickens including some broiler...
Madbouly, H.M; Orkhan, M.H**; Nagwa El-khoty
...s spread and recorded in chickens (foreign breeds , Balady , and hybrids ) , turkeys, ducks , geese and wild birds (from Giza Zoo) in 20 out of 26 (76.9 %) of the Egyptian governorates within few weeks. The number of confirmed farm cases was 840 farms (layers 327, Broilers 164, Balady 1 17, Saso 86, parents 84, turkeys 32, ducks 29 and grand parents l) and those of rural birds was 129 (home birds 1 19, wild birds 5, abattoir 3, shops sales live birds l, migrat...
Abd Elwanis, N.D.; Abou El Khair, M.A.; Afaf H. Amin; Azab, A. and A.O. Abd El Rahman
...ific pathogen free (SPF) chickens using different doses and estimation of the humoral immune response using the hemagglutination inhibition (HI) test. The vaccine provided a faster and more reliable • immune response against the H5N2 viral antigen after immunizing 3 weeks old chicken with a dose of 0.3 ml/bird, inoculated subcutaneously (s/c) in the nap of the neck, was fairly enough to evoke the required level of immunity. The approach through which this...
Hala M. El-Makaky*•, Sherif, N.A.;Fekria El-Bordiny* and Taha, M.M.*
...l. One hundred and fifty chickens were divided into 3 equal groups. The first group was vaccinated with the prepared SHS vaccine, 2nd group two was vaccinated with imported SHS vaccine, while the third group was kept as non-vaccinated control. Evaluation of the prepared vaccine was done by measuring both cell-mediated and humoral immunity. The results of both T-lymphocyte transformation and ELISA tests revealed that the prepared vaccine induced good protection...
H.A. SULTANl, H.A. HUSSEIN2, and F.F. EL-KHAYAT3
...s in 49-day-old egg-type chickens revealed that the highest mortalities were 53.30% and 36.6% in birds infected with the two field isolates which typed as classic IBDVs. Whereas. in birds infected with three variants IBDVs related to Del/E (two) and RS593 (one). the mortalities 10%, 13.3% and 10%, respectively. Moreover, the pathogenicity of such IBDV field isolates indicated that IBDV related to variants Del/E or RS593 caused severe bursal atrophy (BF) as jud...
Rana Mohammed Ibrahim1*, Haider Mohammed Ali Al-Rubaie2
...spp. parasite in broiler chickens by using thin blood smears Nested and quantitative real time (qRT)- Polymerase Chain Reaction methods in Baghdad city between 1/11/ 2021 and 31/3/2022. Fifty jugular venous blood samples (about 5ml) were collected. The total infection rate of Leucocytozoon spp. in the blood smears was 10% (5/50), which divided into males 6% and in females 4%, while by qRT-PCR was 4% (2/50) with an equal ratio between males and females (2%). In...
Doungnapa Promket1*, Khanitta Pengmeesri1, Jennarong Kammongkun2, Thassawan Somchan1
... hundred Thai indigenous chickens. This study employed PCR-RFLP genotyping of the MTNR1C and NPY genes to investigate their association with egg production traits. The results revealed significant associations between these genes and several egg production parameters on Thai indigenous chicken populations. In the case of the MTNR1C gene, significant effects were observed on WE360d, NE360d, and E/M (P < 0.05). Specifically, Thai indigenous
Farid S. Nassar1,2, Osama A. El-Sayed3, Saidi Ouassaf4, Ahmed O. Abbas1,2*
...FS) inclusion on broiler chickens’ productive performance, slaughter traits, biochemical components, cholesterol profile, stress indicators, and immune status. Four hundred male Cobb500 broiler chickens were grown on floor pens and fed a mash-based corn-soybean diet from one day to 21 days old. From 22-42 days of age, the birds were divided into four experimental groups (10 replicate pens × 10 chicks each) in whi...
Sajjad Khan*, Naila Chand, Abdul Hafeez and Nazir Ahmad
...enic bacteria in broiler chickens. Day-old 200 Ross male chicks were allotted to five groups each subdivided in four replicates with ten birds per replicate. Similarly, five different types of feed i.e., diet A as control while B, C, D and E having 1.5% GA, 30mg/kg BS, 1.5% GA+30mg BS and 0.75% GA+15mg/Kg BS respectively, were offered during 42 days of experimental trial. Statistical analysis was performed by SPSS following CRD design and data was expressed as...
Ahokpossi AGAC1,2*, Salifou CFA2, Youssao Abdou Karim I2, Ameyapoh Y3
...aracteristics of Goliath chickens recently developed by farmers with those of local chickens and exotic chickens commonly found in Benin. Thus, data on carcass characteristics, and sensory meat quality were collected on 120 chickens divided into four groups: (1 and 2), each composed of 20 males and 20 females Goliath chickens
Theresia Nur Indah Koni*, Yeria Banoet, Welhelmina Wahon, Cytske Sabuna, Melkianus Dedimus Same Randu, Yanse Yane Rumlaklak, Tri Anggarini Yuniwati Foenay
...ance of crossbred native chickens. Banana peels were fermented (FBP) anaerobically for 21 days with addition of 10% tapioca flour as an additive. Then banana peels without and with fermentation were analyzed for their nutrient content. This fermented banana peel was then applicated to the crossbred native chicken diet with different levels. There were four treatments and five replications. Treatments were FBP0: diet without fermented banana peels, FBP1: diet w...
Faramin Javandel Soum Sarai1, Mir Daryoush Shakouri2* and Alireza Seidavi3*
...8-d-old Ross 308 broiler chickens were divided into 4 treatment groups with 4 replicates and 10 birds per each by employing a completely randomized design. Broilers were fed on corn-soybean meal basal diets with no additive (control) or added chromium picolinate (400 mg/kg), chromium picolinate (400 mg/kg)+ vitamin C (240 mg/kg) or chromium picolinate (400 mg/kg) +formic acid (0.5%) form 29 to 42 days of age. All supplements significantly improv...
Heni Indrijani*, Asep Anang, Muhammad Farhan Fadilah, Nena Hilmia, Maya Fitriani
...ed using a sample of 319 chickens selected from two different groups. The first group consisted of 13 males and 147 females Sentul Debu chicken aged 11 months, while the second group consisted of 13 males and 146 females Sentul Kulawu chicken aged 14 months. The hatching eggs produced from breeding one line and crossbreeding were hatched for 21 days, resulting in fertility, and hatchability was calculated as a value of the heterosis effect. Each DOC was identi...
Khaeruddin1, Gatot Ciptadi2, Muhammad Yusuf3, Iswati4, Setya Budhi Udrayana4, Sri Wahjuningsih2*
...iently use superior male chickens for mating with multiple females. This study aimed to examine the effect of adding BHT (butylated hydroxytoluene) into the diluent and storage time on the quality of Gaga chicken spermatozoa during liquid storage. The semen was collected using abdominal massage methods and divided into 4 tubes filled with diluent. For the analysis, the diluent selected was egg yolk Ringer’s lactate added with BHT at concentrations of 1, ...
Thobela Louis Tyasi*, Lebo Ngorima, Victoria Rankotsane Hlokoe
...st predict egg weight in chickens. The study aimed to develop a model that can be employed to estimate egg weight from egg quality traits with stepwise regression in the Lohmann brown chicken breed. The study was conducted at Kitamu farm in Ntsima village in Limpopo Province, South Africa. The Pearson’s correlation findings displayed that egg weight (EW) had a highly positive remarkable association (P < 0.01) with egg length (EL), yolk weight (YW), eg...
Raza Ali Mangi1,2, Ali Raza Jahejo1,2, Afrasyab Khan1,2, Meng-li Qiao1,2, Muhammad Farhan Qadir1,2, Mazhar Hussain Mangi3, Shi-xiong Yang1,2, Xin-yu Han1,2, Sheng Niu1,2, Ding Zhang1,2, Ying Wang1,2 and Wen-xia Tian1,2*
...mmon problems in broiler chickens. Chicken erythrocyte is the most abundant cell found in blood vessels, in which host defense resistance (HDR) and pattern recognition receptors (PRRs) play a significant role in the immune response. However, the function of HDR and PRRs in thiram induce TD chicken is not fully known yet. Therefore, this study was designed to evaluate the effect of recombinant glutathione-S-transferase A3 (rGSTA3) protein on HDR and PRRs in the...
Frennada Lu’fatul Azizah1, Osfar Sjofjan2*, Eko Widodo2
... of the ileum of broiler chickens. The BSF (Black Soldier Fly) oil isolation process was initiated using the Soxhlet method. Subsequently, 160 broiler chickens were utilized in the research. An experimental design in the form of a completely randomized design with five treatments and four replications was employed, involving eight broiler chickens per treatment per replication. The feed tr...
Irfan Ullah1, Asad Ullah2*, Tahira Tayyeb1, Rafiq Ullah1, Muhammad Hanif1, Faiza Khan3, Imad Khan2, Raheela Taj4, Fatima Syed4, Shumaila Gul5, Muhammad Sadeeq6, Muneeb Islam7, Arsalan Khan8 and Khudija Ghani9
...) in each group. Broiler chickens were fed with starter and finishing commercial corn based basal diet (BD) with different doses of inorganic Selenium (Se) powder. Group-A was kept negative control provided with BD twice a day. Group-B was positive control group fed with 15mg DEX/kg twice/day. The Group-C was fed with BD+0.2mgSe+15mg DEX/kg. Similarly, Group-D was fed with BD+0.3mgSe+15mgDEX. Group-E was fed with BD+0.4mgSe+15mg DEX/kg. Two chicks were randoml...
Wahid Hussein El-Dabae*, Eman Shafeek Ibrahim, Eslam Gaber Sadek, Mai Mohamed Kandil
...in vaccinated challenged chickens than in non-vaccinated challenged chickens. Quantitation of viral loads following challenge indicated reduction of viral loads shed from birds vaccinated with the attenuated AOAV-1 virus. These preliminary insights collectively underline a new hope for the attenuation of live AOAV-1 genotype VII.1.1 as a vaccine candidate that can be used to control ND outbreaks in Egyptian poultry.
Aatif Masood Ahmad Khan1, Masood Rabbani1*, Arfan Ahmad1, Muhammad Wasim2 and Sohail Raza1
... Coryza (IC), from layer chickens in various farms in Pakistan. A total of 46 isolates were obtained from a total of 244 clinical samples from 122 sick and moribund or dead layer chickens showing signs of IC like slight swelling of infra orbital sinuses and nostril mucus membranes. Two sampling sites were chosen, squeezing the nostrils for live birds and infra orbital sinus for moribund killed with later providing more live ...
Kunlayaphat Wuthijaree1, Pattaraporn Tatsapong1, Sukanya Yung-Rahang2, Prayad Thirawong2, Koonphol Pongmanee2*
...lth problems in backyard chickens and is detrimental to productivity performance. This study evaluated the prevalence and average number of gastrointestinal parasites per chicken (worm burden) in Thai indigenous chickens (Gallus gallus domesticus). A total of, 229 chickens (113 males and 116 females) were investigated in 3 selected districts in central Thailand. Ch...
Feri Eko Hermanto1, Yuli Frita Nuningtyas1, Filoza Marwi1, Fajar Shodiq Permata2, Agus Susilo1, Muhammad Halim Natsir1*
...immune system in broiler chickens. However, understanding how these herbs confer protective effects remains challenging, given the intricate nature of mucosal immunity and its role in maintaining gut health. This study adopts a network biological approach to decode the complex web of protein interactions within mucosal immunity and explore the immunomodulatory potential of bioactive compounds found in these herbs. Biological networks were constructed for gut h...
Hashim Hadi Al-Jebory1*, Mohammed Khalil Ibrahim Al-Saeedi2, Majeed Ajafar1, Nihad Abdul-Lateef Ali1
...h performance of broiler chickens. One day old, 400 Ross 308 broiler chicks were randomly allocated into five random groups, with each group being quadruplicated and 20 birds allocated to each replication. Melatonin was supplemented in the G1 control group. G2 received 10 mg of melatonin per kg of diet, G3 received 20 mg of melatonin per kg of diet, G4 received 10 mg of melatonin per liter, and G5 received 20 mg of melatonin per liter, respectively. All ...
Nadya Indriyani1, Sri Murtini2*, Sri Darwati3, Cece Sumantri3
...lyzed antibodi in IPB-D3 chickens on production performance. Pedigree data and information were collected in generations 3 and 4. In the third generation, as many as 36 chickens (9 males and 27 females) aged 27 weeks and 162 IPB-D3 chicken eggs, in the fourth generation aged DOC and 2 weeks, each 81 samples. Total Immunoglobulin yolk (IgY) observations were made using Enzyme Linked Immunosorbent Assay (ELISA) and antibody ti...
TAHIR IQBAL1, 2, UMER RASHID1* & MUHAMMAD IDREES3, 4
...megaly syndrome (HSS) in chickens. Like other HEV strains, it is single-stranded positive sense RNA virus belonging to Hepeviridae. In the present study we report partial structural homology of ORF3 encoded protein (ORF3P) of Pakistani novel aHEV (Pak naHEV) strain with a kinase 2-amino-4-hydroxy-6-hydroxymethyldihydropteridine pyrophos-phokinase (HPPK) which participates in folate biosynthesis in bacteria. This finding is in agreement with the role of ORF3P a...
Sri Wahjuningsih1*, Ani Atul Arif1, Khaerudin2, Herlina Pratiwi3, Ardyah Ramadhina Irsanti Putri1
...aterial was 10 male Gaga chickens who were 10 months old, healthy, and normal reproductive organs. Semen was collected using massage methods. This study uses a complete random design of factorial patterns with factor I being the equilibration time and factor II being the different thawing conditions. Fresh semen was evaluated macroscopically and microscopically. The sperm used had a motility of over 80%. The semen was diluted Lactate Ringer with 10% egg yolk a...
Ngoc Tan Nguyen1*, Thi Tuong Vi Trang1, Thi Thuy Tien Nguyen2, Tuan Thanh Hoang2, Duc Thoa Nguyen2
...blood samples of 393 VLV chickens. The 24 bp indel/PRL target gene was amplified by PCR and the product was separated by electrophoresis on 2% agarose gel for genotyping. Polymorphism was observed at the 24 bp PRL/indel locus, showing two alleles I and D with frequencies 0.57 and 0.43, respectively and three genotypes (II, ID, and DD) with frequencies 18.3, 49.4, and 32.3%, respectively. Hens with the DD genotype had a significantly (P<0.05) higher ANE than...
Firda Arlina1*, Mangku Mundana1, Linda Suhartati1, Agmelia Jasfaria2
...dah Batu Kokok Balenggek chickens were sexually mature. This study used a survey method, and sampling was carried out using purposive sampling. The parameters observed were qualitative and quantitative phenotypes. The data obtained were analyzed by descriptive statistical analysis. The results showed that the qualitative variety of the Randah Batu Kokok Balenggek chicken reared by the Kokok Balenggek chicken Farmers in Solok Regency, West Sumatra, based on the...
Srisukmawati Zainudin1,3, Hartutik2, Edhy Sudjarwo2, Osfar Sjofjan2*
...gul Balitnak (KUB) local chickens, employing a completely randomized design with four treatments and six replications. The feed treatments consisted of T0 (basal feed), T1 (basal feed with 10% fresh skipjack tuna offal meal), T2 (basal feed with 10% steamed skipjack tuna offal meal), and T3 (basal feed with 10% fermented skipjack tuna offal meal). Data were collected and analyzed using analysis of variance (ANOVA). The results showed significant differences (p...
Marwa R. El-Deken1, Raouf E. Rizk1, Mona R. Ahmed1, Fouad A. Tawfeek1, Hossam A. Shahba1, Wesam A. Fares1, Ayman M. Khalifah2*
...od parameters for Inshas chickens during the late laying period was performed. A total of 330 of Inshas chickens aged 50 weeks were randomly divided into five treatment groups. The birds of control group (Glu 1) were not supplied with Glu acid. Rest birds groups were supplemented with Glu as additional doses (0.4, 0.6, 0.8 and1%) for Glu2, Glu 3, Glu 4 and Glu 5, respectively. Egg production percentage and egg mass for Glu 3...
Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4
...e disease virus (NDV) in chickens at Barishal district of Bangladesh, from November 2021 to October 2022. During the reporting period, a total of 419 serum samples (227 layers and 192 broilers) were randomly collected from apparently healthy, sick and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall sero...
Dilruba Akter Mir1, Md. Sahidul Islam¹*, Mosammat Mahamuda Khatun1, Sharmin Zaman1, Bidyut Matubber², Nazmin Sultana Runa3, Zannatul Ferdous3, Tithe Saha3, Md. Anwar Jahid4
...e disease virus (NDV) in chickens at Barishal district of Bangladesh, from November 2021 to October 2022. During the reporting period, a total of 419 serum samples (227 layers and 192 broilers) were randomly collected from apparently healthy, sick and recovered birds. The haemagglutination inhibition (HI) test was used to analyze the chicken sera samples for determination of the titer of the antibodies against NDV. The test results showed that the overall sero...
Mirwaise Khan1, Rubab Maqsood1, Hamad Bin Rashid2, Shakera Sadiq Gill1, Muhammad Noman1, Rafia Akram1, Muhammad Sajid Hasni1, Haroon Rashid3 and Mamoona Chaudhry1*
...od samples from backyard chickens were collected and were tested for antibodies against Avian Influenza Virus (AIV) (H9N2) by hemagglutination inhibition test according to OIE. It was found that out of 240 collected blood samples, 140 (58.3%; 95% CI: 50.43-69.60) were positive for AIV (H9N2). The findings of the present study indicated that AIV (H9N2) were endemic and widely distributed in backyard poultry in different areas of District Zhob, which is continuo...
Philip O. Akporhuarho1, Ufuoma Godstime Sorhue1*, Adimabua Mike Moemeka2, Onyinye Stella Onwumere-Idolor3, Jonathan Ujomu1, Emmanuel Abadah1, Jennfer Aaron1
...; genes in the spleen of chickens subjected to Aframomum chrysanthum seed meal (ACSM) as a replacement for synthetic antibiotic in broiler production. A total of one hundred and forty-four day-old Aboracre strains of broilers were randomly allotted into four dietary treatments. Treatments one (control) had no ACSM in the diet but was given routine antibiotics medication for broilers. Treatments two (T2), three (T3), and four (T4) had ACSM at 1%, 1.5%, and 2% i...
Saadatin Nurul Jannah1, Edhy Sudjarwo1, Osfar Sjofjan2*
...ditives given to broiler chickens. This study was conducted using a laboratory experiment using garlic peel powder as a material, an active substance content test using a quantitative flavonoid test, and antioxidant activity. Additionally, onion peels are used as a feed additive for broiler chickens to evaluate production performance and blood profile. The material used was 189 one-day-old commercial broiler
Abdel Satar Arafa
...problems. LPAIVs H9N2 in chickens tend to show more respiratory tropism with some strains also show gastrointestinal tropism. The ideal mechanism of transmission among birds varies by host species and virus strain. The respiratory and contact transmission are likely the primary routes of transmission and may partly arise initially as an adaptation to poultry which clearly has implication for zoonotic transmission. Inactivated vaccines are the most common vacci...
Noura K. Al-Suwailem1, Nancy N. Kamel2, Ahmed O. Abbas1,3*, Farid S. Nassar1,3 , Dalia A.A. Elsayed4, Gouda F. Gouda1,5, Hosam M. Safaa6
...hundred one-day-old Cobb chickens and were randomly divided into three groups, each with five replicates of 20 birds. The thermoneutral control group received a basal diet, while the other two groups experienced cyclic thermal stress from day 22 to 42 and were fed the basal diet provided with either 0 or 2 g MLM/kg diet. On day 42, we reported carcass characteristics and evaluated meat yield and quality. Blood samples (n=10) were used for hematological assessm...
Minh Thi Le Bui1, Thanh Minh Le2, Thuan Khanh Nguyen1*
...performance of the local chickens. One-day-old chicks (n=400) were allocated to a completely randomized design with four replicates of five treatments. Chickens in treatment T1 were not supplemented with any powder in the basal diet. Chickens in treatment T2 were supplemented with chlortetracycline with 10 mg/kg feed. Chickens in treatment T3, T4, and T5...
M.J.Al-Saadi*, Jassim E.Q.Al-Musawi
...cal parameters. Broiler chickens (n= 160, Ross 308) were divided randomly into four groups first one was the negative control group which kept without exposure to heat stress or mint treatment, while groups 2-4 kept under heat stress. Group 2 kept under heat stress but without mint treatment, while the groups 3 and 4 exposed to heat stress accompanied with treatments of 3% and 5% peppermint leaves powder respectively in the diet. The experiment lasted for 35 ...
Liyun Chang1*, Yumei Cai1, Chenghui Li1 and Jianhua Qin2*
...n chicken, seven day-old chickens were randomly divided into three groups, namely the DNA microsphere vaccine of pcDNA3-3-1E-PLGA microspheres of Eimeria acervulina oral immunization group, the naked plasmid DNA vaccine pcDNA3-3-1E of Eimeria acervulina oral immunization group and control group, with 30 chickens in each group. The chickens were immunized with a dose of 50μg/chicken, res...
Nguyen Thuy Linh, Nguyen Hoang Qui*
...three treatments with 24 chickens per treatment using a completely randomized design to determine the effect of replacing fish meal (FM) by Hermetia illucens larva meal (HIM) in the diet on growth and health of broilers with three treatments of a 15% replacement of FM by HIM, a 30% of FM replacement by HIM and control treatment without replacement of HIM for FM. The results showed that 30% of HIM replacement in diets improved the growth performance; better bod...
Fitrimawati*, James Hellyward
...ng hens, broilers, local chickens and ducks. The data obtained were analyzed using the Analytical Hierarchy Process (AHP) method. This research produces the leading commodities competitively and becomes the priority to be developed in West Sumatera, such as the beef cattle, goats and also buffalos, broilers, local chickens and ducks. Based on competitiveness concept, the first important priority which must be noticed in the ...
Majid Ali1*, Naila Chand1, Sarzamin Khan1, Shakoor Ahmad2 and Muhammad Tahir3
...nd gut health in broiler chickens. A day-old 270 broiler chicks (Ross-308) were randomly assigned to 6 treatment groups with 3 replicates and 15 birds per replicate. CON (Control) group; M100 (M. oleifera 200mg/L); T100 (T. vulgaris 300mg/L); M50T50 (M. oleifera 100mg/L and T. vulgaris 150mg/L); M75T25 (M. oleifera 150mg/L and T. vulgaris 75mg/L); and M25T75 group (M. oleifera 50mg/L and T. vulgaris 225mg/L). Methanolic extract of both M. oleifera and T. vulga...
Sri Purwanti1*, Wempie Pakiding1, Marhamah Nadir1, Nurhayu2, Kusumandari Indah Prahesti1, Sitti Nurani Sirajuddin1, Jasmal Ahmari Syamsu1,4, Andi Mushawwir3
...and energy metabolism in chickens. This research aims to determine the value of lipid regulation and cardiovascular biomarkers of native chickens given a combination of maggot, indigofera and turmeric. A total of 120, 4-week-old male indigenous native chickens with an average initial weight of 42-45 g were divided into 3 treatment groups and fed diets, namely P0 (basal feed), P1 (basal fee...
Muhammad Choirur Roziqin1, Muhammad Halim Natsir2*, Osfar Sjofjan2
... Old Chick (DOC) broiler chickens with MB 202 type unsexed with an average body weight of 45.03 g with a diversity coefficient of 7.35%, employing a completely randomized design with five treatments and four replications. The feed treatments consisted of T0: basal feed without the use of date pit flour, T1: basal feed with the use of 2.5% date pit flour, T2: basal feed with the use of 5% date pit flour, T3: basal feed with the use of 7.5% date pit flour, T4: b...
Quart ul Ain1, Arfan Ahmad1*, Aftab Ahmad Anjum1 and Mamoona Chaudhry2
...(H9) vaccines in broiler chickens. This study was undertaken to evaluate the comparative efficacy of commercial and self-prepared AIV H9 vaccines for their potential to stop or reduce viral shedding post challenge infection (PCI) and to develop humoral immunity. A total of 5 groups A, B, C, D and E comprising of 20 broiler birds each were made. Groups A, B and C were vaccinated on 6th day of age with imported (Ivac), local (Lvac) and self-prepared (Spvac) vacc...
Kremlin Mark B. Ampode
...ed with 125 Cobb broiler chickens and five treatment groups. The diets included antibiotics (0.0025%) in T1, and groups supplemented with varying OLM levels T2 (1%), T3 (3%), T4 (5%), and T5 receiving no antibiotics and OLM. OLM demonstrated a significant positive impact on growth performance (P < 0.05), with birds in the 3% OLM group achieving the highest live weight (1185.40 grams) and average daily gain (38.01 grams) by day 28. Notably, no significant di...
Duong Thanh Hai1*, Phan Thi Hang1, Nguyen Thi Thuong2, Zábranský Luboš3
... hundred forty crossbred chickens begging at 28 days of age. Chickens were arranged randomly into two groups (control and fermented) with four replications. The results shown that the fermented feed did not affect on carcass yield or quality of meat parameters (p>0.05). However, most amino acids of breast meat were found at a higher content in the fermented group than in the control group (p<0.05). Especially, the conc...
Sariffudin Fatmona*, Abdurahman Hoda, Sri Utami
...family of superior local chickens typical of Ternate City. This research was carried out by crossing chickens resulting from crossing (F1) Elba laying hens with male Bangkok RB chickens with chickens resulting from crossing (F1) Bangkok and female BK Kampung reciprocally. Chickens resulting from this cross are placed i...
Salman K.A.A1, Mohammed Khalil Ibrahim Al-Saeedi2, Hashim Hadi Al-Jebory3*
Le Thanh Phuong1, Pham Tan Nha2*
...e 21 weeks old local Noi chickens were used in this experiment. They were randomly distributed into two treatment groups of 5 replicates (30 hens and 6 cocks) in a completely randomized design. Treatment 1 (G0 generation – beginning generation) included 150 hens and 30 cocks and treatment 2 (G1 generation – the offspring of G0 generation) with 150 hens and 30 cocks. Mating cocks and hens of G0 generation and collecting eggs to incubate for getting ...
Kh. Amber1, S.G. Kotb1, W.A. Morsy2* and W. Khalifa1
...h performance of broiler chickens....
Muhammad Shahkar Uzair1, Muhammad Mushtaq1, Muhammad Shuaib1*, Saqib Nawaz2, Mehboob Ali2, Faiz ur Rehman2, Abubakar Sufyan3, Usman Zeb4, Muhammad Ayaz5 and Muhammad Aamir Khan5
...antibiotic (Neomycin) in chickens against the E. coli infection at the finisher stage. Both in-vitro and in-vivo studies were carried out to evaluate the antimicrobial activities and potency of this combination. A total of one hundred and eighty day-old chicks were randomly allotted to 6 groups i.e. G1 (negative control), G2 (positive control), G3 (standard antibiotic neomycin only), SN-1 (Serratiopeptidase @40g/L+Neomycin@10mg/L), SN-2 (Serratiopeptidase @50m...
Rico Anggriawan1, Widya Paramita Lokapirnasari2, Sri Hidanah3, Muhammad Anam Al-Arif4, Diyah Ayu Candra5
...e performance of broiler chickens in open house cages with additional probiotic nutrition. The research method used is experimental based with a post-test design only for the control group. This study used a completely randomized design (CRD). 30 broiler chickens were randomly assigned to three treatments (P0, P1, and P2) at least six times in each group. The results obtained in this research are giving lactic acid bacteria ...
Ibrahim Ali1,2, Hanan Mohamed Fathy Abdien1*, Wael Kamel Elfeil1, Mohsen Mohamed Zaky ElDemerdash1, Mohamed Ali Zain El-Abideen2,3, Walid Hamdy Kilany2,3
...ific pathogen-free (SPF) chickens were treated as G1 and G2 vaccinated with (H5N8+Gel 01), G3 and G4 with (H5N8+IMS1313) by intranasal (IN) and intramuscular (IM) for each treatment respectively. Each group (G1-G4) was vaccinated twice at day 7 and 21 of age. Meanwhile, G5 treated with (H5N8+ISA70) received the primary vaccination dose intramuscularly only on day 7 of age. Group G6 served as a positive control (untreated and challenged), and G7 served as a neg...
Muhammad Luthfi Hidayat, Istna Mangisah, Lilik Krismiyanto, Vitus Dwi Yunianto*
... and immunity of broiler chickens. A total of 200 Ross strain broilers were arranged in a completely randomized design with 5 treatment groups and 4 replications. The treatments include: T0 (control), T1 (0.1% ELPPP), T2 (0.2% ELPPP), T3 (0.3% ELPPP), T4 (0.4% ELPPP). ELPPP was applied from day 8 to 35. The parameters observed included broiler performance, bacterial populations in the small intestine, small intestine pH, protein digestibility, malondialdehyde ...
Mohammed Khalil Ibrahim Al-Saeedi1, Majeed Ajafar2, Hashim Hadi Al-Jebory2 *
...otal of 120 Lohman brown chickens aged 80 weeks were exposed to force molting for 6 weeks using the fasting program. All laying hens were randomly assigned to five dietary treatments, each containing three replicates (8 chickens/ replicate). Hens in the first group were fed the basal diet without supplementation and served as the control (G1), while those in the 2nd (G2), 3rd (G3), 4th (G4), and 5th (G5) groups were fed the ...
Nguyen Tuyet Giang1,2*, Le Thi Thuy Hang1,2, Phan Phuong Loan1,2 and Le Thi Thuy Loan1,2
...he performance of laying chickens....
Mohamed Ahmed Abaza1, Amany O. Selim2, Mona Abdallah3, Shimaa A.E. Atwa4, Hala El Daous5, Mona Abd-Allah Abd-Elrehim6, Mohamed M.S. Gaballa7, Reda R. Fathy1*
...ic health impact on both chickens and human. Antibiotic resistance as well as antibiotic residues in chicken meat are some of the negative outcomes of the traditional antibiotic-based approach to prevent and control bacterial infections. Therefore, the main goal of current investigation was to control the drug-resistant E. coli O6 infection using nano-production of zinc oxide (ZnO-NPs) in both in vitro and vivo studies. ZnO-NPs was applied in one day old speci...
Rand K. Abbas1, Zahraa S. Hadi1*, Zainab A. Hussein2, Sajad K. Matooq3
..., and WBC), sixty mature chickens were employed. Blood samples (1 ml) were taken from each chicken’s jugular vein and placed into an ethylene diamine tetraacetic acid test tube. The tube was then gently shaken. The results were obtained using the Cell-Dyn Ruby equipment. The dissected intestinal portions of the sacrificed chickens were extracted in order to look at the pathological alterations, and the
Md. Gausur Rahman1*, S.M. Harun-Ur-Rashid1, Md. Golam Azam1, Md. Ahsan Habib2, Golapi Rani Devsharma1
...xamined birds, 56 Sonali chickens (28.00%) were infected with gastrointestinal parasites including Ascaridia sp., Raillietina sp. and Eimeria sp. Among these parasites, Eimeria sp. (15.5%) was the most prevalent (P<0.001) parasite, followed by Ascaridia sp. (11.5%) and Raillietina sp. (1.00%), respectively. A significantly (P<0.01) higher prevalence of gastrointestinal parasites was recorded among Sonali chicken of 5-9 weeks of age (37.39%) than 1-4 week...
Faiza Ghazanfar1, Masood Rabbani1*, Aamir Ghafoor2 and Muhammad Hassan Mushtaq3
...c C. jejuni from broiler chickens. A total of 375 cloacal swab samples were collected by systematic sampling method of commercial broiler farms in and around Lahore and examined for the presence of thermophilic C. jejuni. Their prevalence was found to be 53% (200/375). Confirmation was done by biochemical and molecular (Polymerase chain reaction) testing methods. Phylogenetic tree analysis was also performed for genetic identification of the species. in vitro ...
Rico Anggriawan1*, Widya Paramita Lokapirnasari2, Sri Hidanah3, Muhammad Anam Al Arif4, Diyah Ayu Candra5
...stry, especially broiler chickens, often uses antibiotics to cure bacterial infections and secondary infections caused by bacteria. One of the microorganisms that cause digestive and respiratory diseases in broiler chickens is the bacteria. Treatment of bacterial infections with antibiotics is increasing every year. Broiler chickens infected by gram-positive and gram-negative bacteria are ...
Ridha Tunnisa1, Muhammad Ihsan Andi Dagong2*, Sri Purwanti2, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2
...he (IGF-1) gene in Alope chickens and its relationship to the growth traits of Alope chickens. A total of 120 animals consisting of 52 cocks and 68 hens, were included in this study. Chickens were kept in individual cages to observe growth traits, including initial body weight, final body weight, daily body weight, body weight gain, feed consumption, and feed conversion ratio. Growth trait...
Nguyen Thi Ngoc Trang1, Nguyen Thi Thu Hong2,3*
...t on 120 Minh Du broiler chickens from 4 to 14 weeks of age. The experiment comprised 4 treatments with control (no addition), experimental treatment GARL 1, GARL 2, and GARL 3 with adding 1%, 2% and 3% of fresh garlic, respectively. The experiment used 10 chicks per unit and 3 replications. The results showed that the increased body weight of Minh Du broiler chicken in experimental treatments was higher than control treatments (P<0.05). Dietary supplementa...
Manel Ben Larbi1*, Ameni Askri1, Mariem Saidani1, Naceur M’Hamdi2, Ibrahim El Akram Znaïdi3, Nadia Ben Braiek4 and Hajer M’Hamdi5
...align: justify;">Broiler chickens have been selected for their rapid growth rate as well as for their high protein quality. They are reared in intensive systems at high stocking density ranging from 30 to 40 kg live weight/m2. The industry’s drive to ever faster growth rates has an impact on broiler health and welfare such as painful leg disorders and heart failure in broiler chickens and hunger due to severe food rest...
Jennarong Kammongkun1, Doungnapa Promket2*
...phology traits in 281 PH chickens using PCR-RFLP genotyping. The analysis revealed significant associations between growth performance and morphology traits in PH chickens. The correlations between average daily gains (ADG4 to ADG16) and body weights (BW0 to BW16) ranged from 0.33 to 0.99, indicating a consistent upward trend over time. The high body weight (HBW) group exhibited superior growth performance and morphology tra...
Khawla Abdul- Aziz Salman1, Mohammed Khalil Ibrahim Al-Saeedi2, Hashim Hadi Al-Jebory3*, Rasha Fajer Al-Jebory4
Mona F. Shousha1*, Aml M. Ragab2 and Salwa M. Helmy1
... in the diseased broiler chickens, with a possibility of human infection and treatment failure. Therefore, it is highly recommended that developing countries drastically reduce the overuse of antibiotics in poultry....
Dheyaa Ali Aljuhaishi*, Firas Hussain Kadim Albawi
... water. In the group G3, chickens were orally inoculated with E. coli at a dose of 0.5 ml containing 7.5x106 CFU/ml and were treated with both combination bacteriophage 3.2×108PFU/g with feeding and enrofloxacin 100mg/kg and neomycin 200mg/kg orally through drinking water. The G4 acted as control group, where chickens were orally inoculated with E. coli at a dose of 0.5 ml containing 7.5x106CFU/ml whereas G5 control gr...
Yasser Asaad Hameed Al-Shareef, Firas Hussain Kadim Abawi*
Kamal Ahmed El-Shazly1, Amira Abd El-Latif1, Nagwa Elhawary2, Asmaa F. Alswaf1, Foad Farrag3, Samy Sayed4, Mohamed El-Sharnouby5 and Mustafa Shukry6*
...r combination to broiler chickens experimentally induced cecal coccidiosis. For this study, divided randomly one hundred broiler chicks at 15th-day old into equal five groups (20 each). kept G1 as control negative; G2, non-treated infected; G3, infected treated with cashew oil (1.5ml/liter drinking water); G4, infected treated with toltrazuril (2.5ml/liter drinking water); G5, infected treated with a mixture of cashew oil at a dose of 1ml and toltrazuril 1.25m...
Abeer S. Hafez1, Shereen M. Aly1, Dalia M. A. Elmasry2*, H. A. Hussein3, Marwa A. Abdelmagid4
...thological conditions in chickens. The size, uniformity, and spherical shape of PN were measured at 4.065 ±1.12nm, with a polydispersity index of 0.021 which allows for easy blood vessel passage and renal clearance, fresh preparation without adding stabilizer cause zeta potential was recorded at -10 mV. Half maximal inhibitory concentration (IC50) of PN efficacy was found to be 111.5 ug/mL and had a functioning group-specific fingerprint that showing di...
Diaa El-Boraey1, Mousa A. Ayoub1, Wael K. Elfeil2*, Said Ibrahim Fathalla3, Ibrahim Said Abu-alya3, Mohamed Abaza4, Ahmed R. Gado5, Mobarez A.A.6, Nehal K. Alm Eldin6
... vaccine (H9) in broiler chickens. Four groups of chickens were established as follow: a negative control, a positive control (vaccine only), a group receiving the vaccine with amikacin, and a group receiving the vaccine with gentamicin. Hemagglutination inhibition (HI) titers were measured to assess antibody responses in addition to performance parameters. The obtained results demonstrated a reduction in antibody levels aga...
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