Molecular Identification of Chrotogonus spp. (Pyrgomorphidae: Caelifera: Orthoptera)
Molecular Identification of Chrotogonus spp. (Pyrgomorphidae: Caelifera: Orthoptera)
Riffat Sultana1*, Samiullah Soomro1 and Chuan Ma2
ABSTRACT
Genus Chrotogonus has been reported to cause massive damage to agricultural crops where it exists. In past many of its sibling species were misidentified on morphological examination. However, DNA-based species assignments has now made it possible to overcome this barrier. In this study we present CO1 gene data set on the 6 species of Chrotogonus i.e. Chrotogonus (Chrotogonus) homalodemus homalodemus (Blanchard, 1836) with two forms: long winged form and short winged form, C. (Chrotogonus) homalodemus (Blanchard, 1836), C. (Chrotogonus) trachypterus trachypterus (Blanchard, 1836), C. (Chrotogonus) trachypterus robertsi Kirby, 1914 with two forms: long winged form and short winged form, C. (Chrotogonus) trachypterus (Blanchard, 1836) and C. (Chrotogonus) turanicus Kuthy, 1905. However, some closely related species i.e. C. (Chrotogonus) hemipterus Schaum, 1853 with C. (Chrotogonus) h. homalodemus (Blanchard, 1836), and C. (Chrotogonus) senegalensis (Krauss, 1877) with C. (Chrotogonus) t. robertsi Kirby, 1914 were identified as same species i.e. C. (Chrotogonus) h. homalodemus (Blanchard, 1836) and C. (Chrotogonus) t. robertsi Kirby, 1914 respectively. Their lineages indicating negligible genetic divergence and very close resemblance with 99.85% identity between C. (Chrotogonus) h. homalodemus (SWF and LWF) against RID-MMV2XTNY114 and 99.85% identity between C. (Chrotogonus) t. robertsi (SWF and LWF) against RID-MMVW0MTF114. Finally, we discuss the possible taxonomic implication of our DNA sequencing results and point out future research directions.
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