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Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan

Molecular Identification and Characterization of Fruit Flies of Genus Bactrocera (Diptera: Tephritidae) in Pakistan

Jam Nazeer Ahmad1*, Taiba Sharif3, Samina J.N. Ahmad2, Sumaira Maqsood4 and Fariha Zafar3 

1Department of Entomology, University of Agriculture, Faisalabad
2Department of Botany, University of Agriculture, Faisalabad
3Department of Zoology, University of Agriculture Faisalabad
4Institute of Agricultural Sciences, Punjab University, Lahore 

* Corresponding author: jam.ahmad@uaf.edu.pk

 

ABSTRACT

 Molecular identification and characterization of tephritid fruit flies (Diptera: Tephritidae), Bactrocera zonata, Bactrocera dorsalis and Bactrocera cucurbitae was done in Punjab, Pakistan through PCR analysis using mitochondrial cytochrome oxidase I (COI) gene based primers. Phylogenetic analysis was also complemented to differentiate fruit flies identified from other countries of the world. The sequencing results and phylogenetic analysis of collected specimens indicated that sequences of B. dorsalis, B. cucurbitae and B. zonata have 99-100% similarity with fruit flies reported from other countries. This is the first report of molecular identification and characterization of Tephritid fruit flies infesting fruits in Punjab, Pakistan. 

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Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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