Morphological Characterization of Phytophthora infestans and its Growth on Different Growth Media
Morphological Characterization of Phytophthora infestans and its Growth on Different Growth Media
Waqas Raza1*, Muhammad Usman Ghazanfar1, Muhammad Asif2, Ikram-ul-Haq3, Muhammad Zakria4 and Laith Khalil Tawfeeq Al-Ani5,6
Potato Crop Mask of Punjab, Pakistan.
Pathogenicity determination assay (A) Potato crop grown in greenhouse (B) Selected healthy potato plant (C) Healthy leaves brought in laboratory and remove excess moisture on tissue paper (D) Placed detached leaves in moist box (E) Inoculation of pathogen plugs from pure culture of P. infestans (F) Representative inoculated detached leaves.
Mycelium growth (sporulation) on leaves and tuber Sample (A, B, C).
(A-R): Represented pure cultures of P. infestans isolates (showing diversity in colony morphology) of 6 districts from 3 locations each showing different growth patterns (A, B, C) Sargodha. (D, E, F) Sahiwal. (G, H, I) Khushab. (J, K, L) Okara. (M, N, O) Jhang. (P, Q, R) Chiniot.
Data recording for pathogencity (A) Leaves showing lesions on representative inoculated detached leaflets (B) Inoculated leaves showing sporulation (C) Data recorded.
(A-C): Microscopic view of sporangia and Sporangiophore of P. infestans in which zoospores are formed.
(A-F): Morphological characterization of isolates collected from potato growing areas of Punjab during 2017 - Khushab (A), Sargodha (B), Sahiwal (C), Okara (D), Jhang (E), Chiniot (F).
(A-F): Morphological characterization of isolates collected from potato growing areas of Punjab during 2018 - Khushab (A), Sargodha (B), Sahiwal (C), Okara (D), Jhang (E), Chiniot (F).
(A-F): Colony growth of isolates on different growth media collected from potato growing areas of Punjab during 2017 - Khushab (A), Sargodha (B), Sahiwal (C), Okara (D), Jhang (E), Chiniot (F).
(A-F): Colony growth of isolates on different growth media collected from potato growing areas of Punjab during 2018 - Khushab (A), Sargodha (B), Sahiwal (C), Okara (D), Jhang (E), Chiniot (F).
