A total of 17 characters were taken for morphometric studies on Apis mellifera L. and samples were collected from four locations representing two different agro-climatic zones of Himachal Pradesh during 2012. The findings revealed that the significant differences were observed between the populations for all characters except abdomen length, fore wing width and length of trochanter and tibia. Overall, the mean of populations for head height × width, proboscis length, thorax length, abdomen length, fore wing length × width, cubital index, hind wing length × width, number of hamuli, coxa length, trochanter length, femur length, tibia length and metatarsus length × width were found to be 3.19±0.10 × 3.68±0.09 mm, 6.29±0.06 mm, 4.26±0.20 mm, 5.91±0.93 mm, 9.13±0.18 × 3.00±0.08 mm, 2.20±0.36, 6.38±0.14 × 1.80±0.05 mm, 20.88±1.32, 1.10±0.09 mm, 0.79±0.10 mm, 2.45±0.10 mm, 2.91±0.13 mm and 1.92±0.07 × 1.08±0.07 mm. The mean values of important characters like, proboscis length, fore wing length and cubital index were compared with available data of A. mellifera. The mean proboscis length and fore wing length were found to be near to A. mellifera ligustica and A. mellifera meda whereas mean cubital index was near to A. mellifera caucasica and A. mellifera anatoliaca.