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Mutational analysis of Forkhead box P3 gene in Pakistani Human Immunodeficient Virus Patients

Mutational analysis of Forkhead box P3 gene in Pakistani Human Immunodeficient Virus Patients

Aqsa Javaid and Nageen Hussain*

Department of Microbiology and Molecular Genetics, Quaid-e-Azam Campus, Punjab University, Lahore

*  Corresponding author: nageen1704@hotmail.com 

 

ABSTRACT

Human immunodeficiency virus (HIV) becomes one of the most severe endemic viruses that advance around the world in a deadly manner. It is a serious issue in Pakistan as well and so far 130,000 HIV cases in Pakistan have been identified by the National AIDS Control Programme. Nuclear transcription factor forkhead box P3 (FOXP3) gene is involved in tolerance mechanism so failure of tolerance can lead to mutations in this gene. The main objective of this project was to analyze the possible mutation especially in FOXP3 gene exon 1 that may clarify the reason of reduction of T regulatory cells (Tregs) due to HIV/AIDS. A total of 25 HIV patients were chosen from the Institute of Public Health on the basis of confirm HIV infection and 25 healthy controls as well. First genomic DNA was extracted from the peripheral blood and then amplified by using specific designed primers. Gradient PCR was performed and the product length was 197 bps which was further analyzed on 1% agarose gel. Sequencing was done through genetic analyzer (3500 ABI). No mutation was observed in FOXP3 gene exon 1of Pakistani HIV patients.

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Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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