Nodakenin Inhibits Aurka-Cxcl5 Axis Induced Autophagy and Apoptosis in Non-Small Cell Lung Cancer and Promotes Radiosensitivity of Cancer Cells
Nodakenin Inhibits Aurka-Cxcl5 Axis Induced Autophagy and Apoptosis in Non-Small Cell Lung Cancer and Promotes Radiosensitivity of Cancer Cells
Xiaoming Wang1, Rui Tian2, Kunpeng Duan3 and Xu Zhang4*
ABSTRACT
The objective of this study was to explore the role of nodakenin inhibiting AURKA-CXCL5 induced autophagy, cell death and promoting radiosensitivity in non-small cell lung cancer (NSCLC). The expression of AURKA-CXCL5 was determined in HBE, A549, H460 and H1299 cells. H460 cultured cells were divided into two groups: the nodakenin group (experimental group), and the control group. AURKA and CXCL5mRNA expressions were detected by qRT-PCR in HBE and NSCLC cell lines. qRT-PCR was applied to detect the effect of Angelica decursiva on AURKA/CXCL5. LC3-II, P62 and the apoptosis-related factor expression were analyzed by Western blotting. The cell migration and invasion ability was detected by transwell assay. After the cells were irradiated with 4Gy and 8Gy for 24h, the sensitivity of the cells to radiotherapy was detected by MTT assay. Compared with HBE cells, AURKA and CXCL5 mRNA expressions were increased in A549, H1299 and H460 (P<0.05). The AURKA and CXCL5 mRNA expressions in the experimental group were lower than in the control group (P<0.05). Compared with the control group, the LC3-II in the experimental group increased (P<0.05). The P62 protein expression in the experimental group was declined (P<0.05). Compared with the control group, the Bax and Caspase-3 in the experimental group was added (P<0.05). The Bcl-2 in the experimental group declined (P<0.05). The number of cell migration and invasion in the experimental group decreased compared wih the control group (P<0.05). After the cells were irradiated with 4Gy and 8Gy for 24h, the cell viability of the experimental group was lower than the control group (P<0.05). Nodakenin regulates autophagy and apoptosis of NSCLC by inhibiting the activation of the AURKA-CXCL5 axis, enhancing the radiosensitivity.
To share on other social networks, click on any share button. What are these?
