Nuclear and Cytoplasmic Maturation of In Vitro Matured Denuded Oocytes in the Presence of Gonadotrophins, Oestradiol and Insulin in Nili Ravi Buffalo
Beenish Shahid1* and Muhammad Ijaz Khan2
1Department of Zoology, King Abdullah Campus, University of Azad Jammu and Kashmir, Muzaffarabad, 13100 Pakistan
2Livestock Development Research Centre, Muzaffarabad, Azad Jammu and Kashmir, 13100, Pakistan
Fig. 1.
Photomicrographs of fully grown Nili Ravi buffalo oocytes during in vitro culture: (a) An intact germinal vesicle (indicated by arrow) with a single prominent nucleus arrested in dictyate stage of the first meiotic prophase. (b) Oocyte undergoing GVBD showing complete dissolution of the GV with only a small remnant of the nucleolus. (c) MII oocyte arrested at metaphase II stage and omitted a polar body (indicated by arrow). (d) Degenerated oocyte showing shrinkage of ooplasm. Photographs were taken from an inverted phase contrast (Nikon) microscope (objectives: a-d, X20).