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Polyethylene Glycol (Peg) Mediated In Vitro Characterization of Sugarcane (CP-77/400) Calli and Regenerated Plantlets

Polyethylene Glycol (Peg) Mediated In Vitro Characterization of Sugarcane (CP-77/400) Calli and Regenerated Plantlets

Ayesha Gul1, Mohammad Sayyar Khan1*, Mazhar Ullah1 and Iqbal Munir3

1Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Pakistan; 2Department of Agronomy, The University of Agriculture Peshawar, Pakistan; 3Biochemistry Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Pakistan.

 
*Correspondence | Mohammad Sayyar Khan, Genomics and Bioinformatics Division, Institute of Biotechnology and Genetic Engineering (IBGE), The University of Agriculture Peshawar, Pakistan; Email: sayyarkhankazi@aup.edu.pk 

Figure 1:

Effect of PEG stress on morphology of sugarcane calli after 30 days of stress application. Calli morphology changed with increasing PEG concentration. Control (0%) (A); 2.5% PEG (B); 5.0% PEG (C); and 7.5% PEG (D).

Figure 2:

Effect of PEG stress on morphology of sugarcane calli after 60 days of stress application. Calli morphology changed with increasing PEG concentration. Control (0%) (A); 2.5% PEG (B); 5.0% PEG (C); and 7.5% PEG (D).

Figure 3:

Regeneration of PEG selected and non-selected sugarcane calli into plantlets. Calli regeneration on MS media (A); growth of plantlets from control calli on media with 0% PEG (B); growth of plantlets from selected calli on media with 7.5% PEG (C), growth of plantlets from non-selected calli on media with 7.5% PEG (D).

Equation 1
Equation 2
Equation 3

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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