Polymorphisms of Somatostatin Receptor (SSTR-1) Gene and its Relationship with Carcass Traits in Iraqi Awassi Sheep
Polymorphisms of Somatostatin Receptor (SSTR-1) Gene and its Relationship with Carcass Traits in Iraqi Awassi Sheep
Dhurgham K. Seger1, Rahman H. H. Al Qasimi2, Azhar A. Jaffar3, Salah H. Faraj4, Ahmed I. Ateya5*
ABSTRACT
The current study was conducted on 100 lambs of Iraqi Awassi sheep, slaughtered at the age of 10 months. DNA extraction and PCR reactions were carried out at the biology sciences laboratory of the Marshes Research Center/Thi-Qar University. Sequencing technology was used to detect mutations in SSTR1 gene. The results of the multiple alignment analysis of the sequence of SSTR1 gene indicated the presence of one mutation 264 A>G. The obtained mutation produced three genotypes, namely AA, AG, and GG. The frequency of the genotypes AA, AG and GG were 0.50, 0.17 and 0.33, respectively. While the frequency of the A and G alleles were 0.58 and 0.42, respectively. There were significant impacts of SSTR1 gene polymorphisms and the weights of carcass characteristics including heart and liver weight alongside recovery rate. Meanwhile, there was no significant effects between polymorphisms of SSTR1 and the remaining carcass traits including live weight, carcass weight, clearance percentage, and spleen and kidneys weights. This study highlights the possible efficiency of using SSTR1 as candidate marker for carcass traits in sheep.
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