Usman Ijaz1, Muhammad Sajid Tahir2, Khalid Abdul Majeed3*, Shahid Iqbal4, Iffat Huma5, S. Firyal6, Ijaz Ahmed7, Shahid Chohan8 and Aamir Riaz Khan9
...ed for single nucleotide polymorphisms (SNPs). SNPs were observed on three loci of the COI gene as compared to reference sequence of Panthera pardus pardus. The specimens found in Pakistan were found to be closely related to Panthera pardus orientalis (Amur Leopard in South eastern Russia) and Panthera pardus japonensis (North Chinese Leopard), with all of these three speculated to be evolved from Panthera pardus pardus in not-so-fa...
Pan-pan Guo1, Wei Liu2, Yan Li2, Rui-yu Ma2, Wang Zaigui1*, Kai Zhan2*, Jun-ying Li2 and Sheng-nan Liu2
...were to identify genetic polymorphisms of the huntingtin (HTT) gene and to further investigate its association with production performances in two Anhui local chicken breeds. A total of 800 chickens (400 Huangshan black chickens and 400 Huainan partridge chickens) of similar age and weight were evaluated in the present study. We extracted DNA from blood samples and analyzed polymorphisms of HTT gene as wells as possible asso...
Süleyman Kök1*, Sertaç Atalay1, Hasan Semih Eken2 and Mustafa Savaşçi3
...CAPN1 316 and CAPN1 4751 polymorphisms were found to be 0.6038, 0.1115 and 0.5654, respectively. CC genotype frequencies in the three polymorphic areas in TGC were calculated as 0.365, 0.012 and 0.320, respectively, while the distributions of the marker genotypes, except for CAPN1 4751 were found to be significant (P<0.05). The positive alleles of SNPs in Calpain and Calpastatin genes and all of their genotype combinations, were also found to be carried by ...
Shazia Irfan1*, Asima Rani1, Muhammad Arshad2 and Razia Bashir1
...dants enzymes because of polymorphisms in antioxidant genes and variations in levels of metals and minerals creates oxidative stress may play some role in progression and pathogenesis of RA. The objective of this study was to investigate the role of Superoxide dismutase 1 (SOD1) gene polymorphism [rs2070424 (–251A/G)] in RA and to compare the levels of metals and minerals among its various genotypes in RA patients. A total of 400 individuals including ag...
K.N. ArulJothi1, M. Abinaya1, B. Suruthi Abirami1, Melvin George2, S. Elangovan3 and A. Devi1*
...tasis. The effect of the polymorphisms in the gene have varying influences on lipid levels and development of cardiovascular disease (CVD) in different populations. In this study, we investigated the association of rs5888 polymorphism with serum lipid levels and CVD risk in an Indian population. A total of 412 samples which included 148 myocardial infarction survivors, 162 Normolipidemic healthy controls and 102 patients with hypercholesterolemia in a I...
Jakub Jan Skorupski
...9), and three di-allelic polymorphisms - g.616G>C, g.846A>G and g.931C>T (PIC>0.2400). The recognized SNPs enrich the database of single nucleotide polymorphisms for American mink.
...
Sadia Munir1a, Asif Nadeem1a*, Maryam Javed1, Masroor Ellahi Babar2, Tanveer Hussain2, Wasim Shehzad1, Rajput Zahid Iqbal3 and Sidra Manzoor1
...t of primers. A total 15 polymorphisms; 12 in intronic and 3 in exonic region, were identified. The sequences of the amplified Pit1 gene fragments were aligned with the help of BLAST for SNPs identification. This is a first report toward genetic screening of this gene at molecular level in Sahiwal cattle of Pakistan. No work has been reported on this gene in Sahiwal cattle. In this study, a new set of single nucleotide polymorph...
Ghulam Abbas1,Asif Nadeem1,*, Masroor Ellahi Babar2, Tanveer Hussain2, Muhammad Sajid Tahir3, Wasim Shehzad1, Rajput Zahid Iqbal3, Muhammad Tayyab1 and Maryam Javed1
...ed and single nucleotide polymorphisms were identified. Bioinformatics tools were applied for construction of phylogenetic tree and genetic diversity analysis. Lower genetic diversity was observed. The finding of this research is prerequisite for future research.
...
Selçuk Kaplan1,* and Sertaç Atalay2
... and Lep/BcnI polymorphisms were examined by polymerase chain reaction- restriction fragment length polymorphism method. The single nucleotide polymorphism (SNP) in the regulatory region of the IGF1 gene was detected by amplification of the 294 bp region using specific primers and cleavage with the BfoI enzyme. Allele frequencies of A and B were found with 0.915 and 0.085 respectively. The genotype frequencies of IGF1 gene were ...
Wang-Dui Basang1, Tian-Wu An2, Luo-Bu Danjiu3, Yan-Bin Zhu1, Shi-Cheng He3, Xiao-Lin Luo2, Wei-Wei Ni4, Xiao Wang4, Shu-Zhu Cheng4, Jian Wang4 and Guang-Xin E4,*
...ing 54 single-nucleotide polymorphisms and 3 single-nucleotide copy number variants, and 59 haplotypes were detected. The number of haplotypes within the population ranged from 17 (SN and JL) to 28 (DX). The haplotype diversity ranged from 0.9420 (SN) to 0.9770 (NR). The highest nucleotide diversity was found in the JL population (0.01479), whereas the lowest was found in the SN (0.00894) population. Phylogenetic analysis revealed that these four populations s...
S. Justin Carlus1,*, Atiyeh M Abdallah1,2, Abdulhadi H. Al-Mazroea1, Mazen Khalid Al-Harbi3 and Khalid M Al-Harbi1
...tase (MTHFR) gene polymorphisms and CHD and DS co-inheritance in patients from Al Madinah, Saudi Arabia. MTHFR rs1801133 and rs1801131 polymorphisms were genotyped in 99 CHD patients with or without DS and 126 ethnically matched controls by allelic discrimination. Of 99 patients with CHD, 26 had DS. MTHFR rs1801133 and rs1801131genotypes and alleles were not significantly different between controls and C...
Zhen-Yang Wu1, Guang-Xin E2,*, Hui Ran1, Da-Hui Wang1 and Tian-You Yang1
...g four single-nucleotide polymorphisms and 59 parsimony-informative sites, and 18 haplotypes were detected. The number of haplotypes within populations ranged from one (WS and DJ) to six (JK). Haplotype diversity ranged from 0.000 (DJ and WS) to 0.793 (JK). The largest amount of nucleotide diversity was found in the SQ population (0.03111 ), whereas the smallest amount was in the DJ and WS (0.00000) populations. Phylogenetic analysis revealed that these 10 pop...
Amna Younus1,3, Mariam Faiz2 and Abida Yasmeen1,*
...qI single nucleotide polymorphisms (SNPs) genotypic frequency among different clinico-pathological features in Pakistani breast cancer patients. Newly diagnosed 300 breast cancer women from different areas of Punjab visiting Institute of Nuclear Medicine and Oncology Lahore were included in this study. Different clinico-pathological features were described. Vitamin D levels were determined and VDR FokI and TaqI SNPs were analyzed. Among diffe...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Shahid Nabeel1 and Abu Saeed Hashmi2
...dentification of genetic polymorphisms in genes enable us to estimate biological similarities and obtain a better perspective of quantitative traits that play key roles in development and regulating growth. The present study was conducted to characterize genetic variability in the growth hormone (GH) and growth hormone receptor (GHR) of Camelus dromedarius. GH is secreted and synthesized from somatotroph cells of the anterior pituitary lobe and plays si...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
...ssion. Single nucleotide polymorphisms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, whether the 3’-untranslated region (3’-UTR) of the MyD88 gene is associated with resistance to Salmonella Pullorum infection still remains unknown. In this study, a total of eight SNPs, includ...
Wenping Hu1, Xiangyu Wang1, Xiaodi Sun2, Ran Di1, Qiuyue Liu1, Zhangyuan Pan1, Xiaofei Guo1, Xiaohan Cao1, Jinyu Wang2, Yingjie Zhang3, Mei Jin4, Yuze Yang5 and Mingxing Chu1*
...lationship between these polymorphisms of TAC3 and the litter size of Small Tail Han sheep was not found.
...
Maria Khalid1, Tanveer Hussain1*, Zahid Farooq2, Kamran Abbas1 and Masroor Ellahi Babar1
...re and single nucleotide polymorphisms (SNPs) were identified. The boot strapped Neighbor joining tree constructed from MEGA7 explained the genetic relationships of A.chukar with related members of Phasianidae family. The phylogenetic analysis also showed the genetic positioning of A. chukar with respect to other different reported species as well. The study gave us useful genomic information about genetic diversity in A. chukar and its ph...
Guang-Hui Tan, Yi-Yu Zhang*, Yuan-Yu Qin, Lei Wu and Jie-Zhang Li
...tigate the effect of the polymorphisms of CYP7A1 gene on lipid traits in Tianzhu Black Muscovy (Cairina moschata). We detected two novel silent mutations, CDS 216 A>G and CDS 681 T>A in exon 2 and exon 3 of CYP7A1 gene, respectively, and both SNPs changed DNA single strand conformation. The A and T allele of CDS 216 A>G and CDS 681 T>A was dominant allele, and its frequency was 0.554 and 0.800, respectively. Each SNP resulted in three genoty...
Inga Kowalewska-Łuczak1 and Ewa Czerniawska-Piątkowska2,*
...leles for the individual polymorphisms studied was the following g.18174 A>G NCF4 gene A-0.807; SAA2 c.-84 G>C G
Yifan Ni1, Jian Liu1, Fen Wu1, Jianfeng Cai1, Jinzhi Zhang1*, Jianqing Hua2 and Jiping Fu2
...te the single-nucleotide polymorphisms (SNP) of the Multiple Drug Resistance Gene 1(MDR1) and the Porcine Osteopotin (OPN) gene and their association with reproductive traits in Landrace, Large Yorkshire and Jinhua sows. A total of 316 sows of three different breeds were used to examine the genotypes, and DNA sequencing method was employed to detect the potential SNPs in this study. There were two SNP loci found in these genes total...
Haseeb Nisar1, Usman Pasha1, Kiran Hanif1, Rizwan Abid1, Rehan Ahmad Khan Sherwani2, Haja N. Kadarmideen3 and Saima Sadaf1*
...nt-size: small;">Genetic polymorphisms in the interleukin 6 gene (IL-6) and its promoter regions have extensively been studied because of their potential role in the pathogenesis of rheumatoid arthritis (RA). Due to the confounding results, reported in several studies, we performed the systematic literature search on IL-6 -572 G/C polymorphism (rs1800796 SNP) and assessed its association with RA in the overall world population, and in the subgroup analy...
Q. Sun1, Q. Liu1, R. Di1, Y. Wang1, S. Gan2, S. Liu2, X. Wang1, W. Hu1, X. Cao1, Zh. Pan1, X. Guo1, Y. Yang3, H.E. Rushdi4* and M. Chu1*
...ion of single nucleotide polymorphisms (SNPs) of sheep THRSP gene and their association with fat deposition were investigated using Altay and White Suffolk sheep. In addition, the messenger RNA expression profiling of THRSP in fat-tailed and thin-tailed breeds was compared among 8 different tissues. Four SNPs have been detected in position g. 205A>C, c. 52C>T, c. 364A>T and c. 1031C>T of THRSP gene. Genotyping method was used t...
Shuanping Zhao, Lei Xu, Hai Jin and Yutang Jia*
... PCR-RFLP methods, three polymorphisms (g.1500G>A, g.1694T>A and g.2213C>G) were identified and genotyped in our population (n=298). Genetic diversity analysis showed that g.1500G>A and g.1694T>A belonged to an intermediate level of genetic diversity (0.25<PIC<0.5), and SNP g.2213C>G belonged to a low polymorphism level (PIC<0.25). LD (Linkage disequilibrium) analysis showed that SNP g.1694T>A and g.2213C>G had a strong linkage...
Syahruddin Said1*, Widya Pintaka Bayu Putra1 and Dani Nur Arifin2
...tudy was to identify the polymorphisms in the exon 11 of bLHR/HhaI gene (SNP g.1553C>T) and its association with service per conception (S/C) of Pasundan cows of West Java, Indonesia. Total of 147 Pasundan cows were used in this study. The PCR-RFLP analysed three genotypes of CC (0.11), CT (0.57) and TT (0.32) were detected in the Pasundan bLHR/HhaI gene with T allele as the dominant allele (0.61). Observed (Ho) and expected (H...
Dilara Devranoglu, Ilksen Tavaci, Gonul Filoglu and Ozlem Bulbul*
...mall;">Single nucleotide polymorphisms (SNPs) are the most abundant genetic markers in the human genome. SNPs are used in forensics for predicting the externally visible characteristics of a given individual based on a sample of DNA alone. In this study, we tested degraded DNA samples to determine the eye color prediction accuracy of the IrisPlex system. We used five years old bloodstains and UVC exposed (30 and 60 min) DNA samples. PCR and electrophoresis wer...
Yongyun Zhang1, 2, Xinyang Fan1, Fangting Zhou1, Weizhen Li3, Yina Ouyang1,4 and Yongwang Miao1*
...in dairy cattle, but the polymorphisms of the CSN1S1 gene have not been fully understood in buffalo. In this study, the polymorphisms in coding sequence (CDS) of the CSN1S1 gene for river and swamp buffalo were detected using PCR product direct sequencing. The CDS for both types of buffalo was the same in length, which contained 645 nucleotides and encoded a peptide composing of 214 residues. A total of 5 singl...
Muhammad Naveed1, Asif Nadeem1,2*, Maryam Javed1, Muhammad Fahad Bhutta3 and Ruqayya Bint Khalid1
...entify single nucleotide polymorphisms and its effect on evolutionary divergence. Nine polymorphisms, six in intronic and three in exonic regions, were identified in STAT5A gene. One exonic polymorphism G→A, in exon 5, was significant that causes a non-synonymous amino acid change from Serine (S) to Asparagine (N). Further, 64 genetic variants were also identified in STAT5A gene and these variants were due...
Zeynep Sonmez1 and Memis Ozdemir2,*
...udy was to determine new polymorphisms in GDF5 (growth differentiation factor 5 (CDMP-1)) and EPS8 (epidermal growth factor receptor substrate 8). After the PCR analysis of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in t...
Zhongyong Wu1, Wenzhu Li2, Chunxia Ni1, Zhuolin He1, Xiao Hou1 and Wanxia Pu1*
...o understand the genetic polymorphisms of dairy-sourced Staphylococcus aureus, we conducted genotyping of Staphylococcus aureus isolates from milk samples collected from cows with clinical mastitis using the random amplified polymorphic DNA (RAPD) method with three oligonucleotide primers. In total, 177 Staphylococcus aureus isolates were obtained from dairy farms in 5 different provinces in China, including Guizhou, Inner Mongolia, Shangh...
Nida Sadaqat1, Abdul Wajid2, Quratul Ain2, Muhammad Zia Akbar2, Farkhanda Manzoor4, Muhammad Sarwar Khan1, Nasir Ali3, Masroor Ellahi Babar3 and Tanveer Hussain3*
...ence of PRNP gene polymorphisms. We analyzed the polymorphisms in PRNP gene sequences in 49 wild Punjab urial (Ovis vignei punjabiensis). Four novel amino acids polymorphisms (p.Q149E, p.Q155E, p.Y228L and p.L253F) were detected in PRNP gene. The urial was monomorphic at codon 149 and 155 and polymorphic at codon 228 and 253. These amino acid
Iqra Mobeen1*, Rabia Arif1*,Maimoona Ilyas1, Siu Fai Lee2 and Muhammad Saleem1
...mall;">Single nucleotide polymorphisms (SNPs) are one of the most common and abundant class of molecular markers present in the genome of many organisms. The current study represents the first attempt to investigate the natural variations in the RK-MTases genes; Ribosomal N-lysine methyltransferase1 (RKM1) and Ribosomal N-lysine methyltransferase4 (RKM4) in Sordaria fimicola using SNP markers. A total seven SNPs in the RKM1 g...
Khanitta Ruangwittayanusorn1, Doungnapa Promket1*, Kamonnate Pimrueng1, Jennarong Kammongkun2
...ls were used to identify polymorphisms of both genes. The frequencies of TT, TC and CC genotypes of DRD2 were 0.05, 0.40, and 0.55, respectively. The frequencies of T and C alleles of DRD2 were 0.25 and 0.75, respectively. Regarding VIP, the frequencies of II, ID and DD genotypes were 0.59, 0.28, and 0.13, respectively. The frequencies of I and D alleles of VIP were 0.73 and 0.27, respectively. The association of DRD2 polymor...
Guangming Xiang1, Ran Di1, Yaowu Wang1, Shangquan Gan2, Shouren Liu2, Xiangyu Wang1, Wenping Hu1, Qiuyue Liu1* and Mingxing Chu1*
... in tail is unclear. The polymorphisms of candidate sheep CEBPA, PRKAG3 and SREBF1 genes and their relationship with fat deposition between fat-tailed (rumped) and thin-tailed breeds were investigated. Two and one SNPs were identified for PRKAG3 and SREBF1 respectively. Genotyping method was used to analyze genotypes among Altay sheep (fat-rumped breed) and White Suffolk (thin-tailed breed) by Sequenom MassArray. For PRKAG3 gene, a c.1744C>T SNP and a c.184...
Ly Thi Thu Lan1, Nguyen Thi Anh Thu1, Lam Thai Hung2, Nguyen Thi Hong Nhan3, Le Thanh Phuong4, Nguyen Trong Ngu3*
...determine how ApoB2 gene polymorphisms are related to growth-related traits and carcass characteristics of Noi chickens. A total of 183 birds were examined in the study. Body weight was recorded individually at days 1, 35, 63, and 91, and carcass parameters were determined at 91 days for all chickens. For determining single nucleotide polymorphisms, the PCR-RFLP method was used with the help of AcyI restriction enzyme. A pol...
Pan Ziyi1, Ambreen Iqbal1, Gao Zhen1, Liu Juan1, Fang Xibi2, Jiang Ping1* and Zhao Zhihui1*
...e that single nucleotide polymorphisms might be used as a molecular marker for marker-assisted selection in beef cattle breeding....
Amira M. Nowier1, Hassan R. Darwish2, Sherif I. Ramadan3, Nadia A. Abo El-Maaty2 and Othman E. Othman2*
...s study were to identify polymorphisms in exon III of the α-lactalbumin gene among three Egyptian goat breeds (Zaraibi, Damascus and Barki) and to investigate the effect of α-lactalbumin gene polymorphism, goat breed, type of birth, season of kidding, litter size and lactation stage on body weight and milk yield traits in Egyptian goats. One hundred and sixty blood samples were collected for DNA extraction; 74 from Zaraibi, 41 from Damascus, and 45...
Zilu Zhang1, Ning Wang2, Ce Hou1, Laiba Shafique1, Saif ur Rehman1, Zhuyue Wu2, Zhiqiang Wang1, Mingsong Wei3* and Kuiqing Cui1*
...uanzhong dairy goats.The polymorphisms sites in PIS region of horned and polled Guanzhong dairy goats were identified, and a total of 30 polymorphisms sites were confirmed (A648T, T652C, G1107C, T2594C, G2820A, G3556A, A3573G, T3621C, A5329T, T5392G, G5878A, C5996T, A6187G, C6228A, G6969T, G7045T, T7057C, C7266G, C7417T, T8327G, T9029G, C10034A, A10846G, del 10861-10862TT, G11842A, G12157A, G12104A, del 12431-12432AT, G12665...
Muneeza Zafar1,2,3, Fazli Rabbi Awan2,*, Munazza Raza Mirza3,*, Sumaira Nishat2,4, Sajid Ali Rajput5 and Imran Riaz Malik1,*
...coding single nucleotide polymorphisms (SNPs) of APOB retrieved from dbSNP using 17 different computational tools and further evaluated the structural impact of these convergent deleterious SNPs on APOB through HOPE. We found 9 missenses, 15 intronic or regulatory region SNPs and 2 were found in miRNA target sites of APOB. Out of these variant, the rs13306194 (Arg532Trp) was found in the conserved region of protein domain, which can potentially disrupt overall...
Saleha Tabassum1, Muneeza Zafar1,2, Munazza Raza Mirza1*, Muhammad Iqbal Choudhary1,3, Ikram ul Haq4, Imran Riaz Malik2, Ambreen Ansari1 and Mehtab Alam5
...f CXCL9 and CXCL10 genes polymorphisms in TB patients of Pakistani population. A total of 260 study participants, including 122 with tuberculosis disease (TB) and 138 healthy control (HC) were included. Out of these 122 TB disease subjects (67%) were diagnosed with PTB (n = 82) and 33% with EPTB (n = 40). Tri and Tetra- ARMS-PCR techniques were used to genotype rs2276886 of CXCL9 and rs5606198 of CXCL10 for all the samples. The results showed significant assoc...
Muhammad Ismail1, Jabbar Khan1*, Zahid Rauf2, Khalid Khan3 and Muhammad Rafi1
...ualize single nucleotide polymorphisms (SNPs); rs9340799 (G>A) and rs2234693 (C>T) in ESR1 gene. The amplified product of 524bp of rs9340799 sequence of ESR1 gene was treated with Xbal restriction enzyme while amplified product of 451bp of rs2234693 sequence was treated with PvuII restriction enzyme. Nine patients were found homozygous and 8 were heterozygous for minor allele while 1 patient was carrying major allele. For rs2234693, all the patients were...
Xin Xing Dong1*, Gai Cui Bai2, Ming Li Li1, Guo Xiang Lan1, Da Wei Yan1, Xiao Yi Wang1, Qiang Chen1 and Shao Xiong Lu1*
... novel single nucleotide polymorphisms (SNPs) at GHRL gene in three different pig breeds, including Duroc, Yorkshire and Landrace, and the association of the novel SNPs with growth traits. PCR-SSCP and sequencing were used to detect the GHRL gene polymorphisms in the present study. Three SNPs were detected, one in the exon 1 (g.3734 G>T), which was synonymous, and two SNPs in intron 4 (g.7691 A>C and g.8523 T>C). Th...
Tinda Afriani*, Endang Purwati, Yurnalis, Jaswandi, Mangku Mundana, Adisti Rastosari, Anna Farhana
...entify single nucleotide polymorphisms (SNP). The samples used in this study were blood samples (n= 70) collected from Pesisir cattle (aged 2-5 years). DNA was isolated and the targeted region was amplified using the polymerase chain reaction (PCR) technique. Then the amplified products were sequenced. The results showed that the population of Pesisir cattle used in this study was polymorphic. There were 5 polymorphisms in t...
Asma Basharat1, Abdul Wajid2*, Andleeb Batool1, Tayyeba Batool3, Abdul Basit4, Kamran Abbas5, Aziz Ullah1 and Mahmood Shaukat6
...dings suggest that TBX22 polymorphisms may responsible for a significant proportion of non-syndromic CP cases in Pakistani population and confirming its importance as a frequent cause of non-syndromic CP across various populations. The robustness of the association between TBX22 and CP is worth further examination in the future across different populations....
Maskur1*, Muhammad Muhsinin1, Sulaiman Ngongu Depamede2
...f BMP15 and BMPR-1B gene polymorphisms and how they affected the prolific traits of Kacang and Boerka goats. This study indicates that the FecXG and FecB mutations were associated with litter size in Kacang and Boerka goat breeds. The FecXG has two genotypes in both Kacang and Boerka goats, namely homozygote wild type ++ and heterozygote mutant carrier G+ with a litter size of 1.56 and 1.65 (Kacang); 1.61 and 1.75 (Boerka), respectively. Meanwhile, FecB mutati...
Xiao Zhang1, Guoqing Shen1, Yang Wang1, Pengdan Huang1,2, Yan Shui2, Zenghong Xu2 and Huaishun Shen1, 2*
...ellite loci all had good polymorphisms. Among them, the polymorphism information content of the 12 loci is greater than 0.5, which is highly polymorphic....
Yuejing Yang1,2, Mengbin Xiang1,2, Zhengshi Zhang3, Minrui Zhou1, Bingjie Hu1, Tingsen Jing1, Zhe Li1, Fenglin Liu1, Hui Luo1, Qinglu Li1* and Hua Ye1*
Zhao Fulin1,2, Liang Chen2, Tao Lin1, Jiang Yanting3, Ouyang Yina3, Hong Qionghua3* and Chu Mingxing1*
...etween single nucleotide polymorphisms (SNPs) in the FGFR1 and EBP41L5 genes and kidding performance of goats. Mass ARRAY®SNP typing technology was applied to genotype the SNPs of FGFR1 and EBP41L5 genes, of which the genetic characteristics of Yunshang Black goat (YS, n=544), Jining Grey goat (JN, n=133) and Liaoning Cashmere goat (LN, n=91) were explored. Then the association was analyzed between FGFR1 and EBP41L5 and kidding performance (litter size, li...
Muhammad Aijaz1, Nasir Mahmood2, Ghulam Mujtaba3 and Imran Riaz Malik1*
...cancer patients. Genetic polymorphisms of the Interleukin-10 (IL-10) gene were studied at position -1082 (G/A) in breast cancer patients in the Pakistani population. Breast cancer patients (n= 200) were recruited from different local tertiary care hospitals along with healthy subjects (n=100) for molecular analysis of the human IL-10 gene mutation at the -1082 (G/A) position. Extraction of genomic DNA was done from patients and healthy subjects. An allele-spec...
Umer Farooq1,2, Nimra Murtaza2, Abubakar Siddique1, Bilal Saleem1, Obaid Ur Rehman1, Nageen Zahra1, Muhammad Uzair1, Muhammad Naeem Riaz1,3* and Muhammad Ramzan Khan1*
...cal to highlight genetic polymorphisms related with economically important traits including milk and meat yield, development, and resistance against diseases. In this present study, our main objective was to identify the deleterious SNPs and their associated genes which possibly disrupt protein’s structure and function, as well as lead to genetic disease. We performed genome wide reference-based sequence alignment and functional annotation to identify de...
Nasir Ali1,2,6, Muhammad Ilyas2,3, Nazia Akbar1,2*, Gohar Rahman2, Shakirullah Khan4, Mujaddad Ur Rehman6, Abdul Samad5, Habib Ahmad1,2 and Bibi Nazia Murtaza7*
... north-western province, polymorphisms in the HVSI region of mitochondrial DNA sequences of 56 ancient samples (1000-3000 old) and 303 modern samples from three different regions (Chitral, Swat and Hazara) were analyzed for hyper variable sequences of mitochondrial DNA for the determination of their maternal affinities. Our results revealed 25% unique haplotypes in ancient samples whereas 28% unique haplotypes have been observed in the modern samples. The most...
Meryem Betmezoğlu1*, Dilek Arsoy2 and Mahmut Çerkez Ergören3
...n different Caprine Prnp polymorphisms were detected including P42= (CCG>CCA), G127S (GGC>AGC), S138= (AGT>AGC), N146S (AAT>GAT), R154H (CGT>CAT), Gln172Arg (CAG>CGG), G172H (CAG>CAT), G172R (CAG>CGT), V179V (GTG>GTT), V187 (CAG>CAT). The three most common genetic markers were P42= 16%), R154H (12%), N146S (8%), respectively. Two different polymorphism, P42= and R154H, were detected in Cyprus native hair goat whereas P42=, G1...
Dhurgham K. Seger1, Rahman H. H. Al Qasimi2, Azhar A. Jaffar3, Salah H. Faraj4, Ahmed I. Ateya5*
...nt impacts of SSTR1 gene polymorphisms and the weights of carcass characteristics including heart and liver weight alongside recovery rate. Meanwhile, there was no significant effects between polymorphisms of SSTR1 and the remaining carcass traits including live weight, carcass weight, clearance percentage, and spleen and kidneys weights. This study highlights the possible efficiency of using SSTR1 as candidate marker for ca...
Skorykh Larisa Nikolayevna1*, Fominova Irina Olegovna1, Kovalenko Dmitriy Vadimovich1, Skokova Antonina Vladimirovna1, Dmitrik Irina Ivanovna1, Kizilova Natalia Igorevna2 and Violeta Caro Petrovic3
Kristina Morkūnienė*, Rūta Insodaitė, Laimutis Kučinskas, Renata Bižienė
...ion of three Mblk-1 gene polymorphisms to the resistance to V. destructor mites. This case–control study involved 117 DNA samples that were genotyped for three single nucleotide polymorphisms (SNP) using the real-time polymerase chain reaction method. Statistical analysis was performed with SPSS Statistics 20 and PLINK software. SNP at position 7454459 (Asn → Thr) of Mblk-1 gene mutant allele A was more common in ...
Muhammad Nauman Arif1, Muhammad Mansha1* and Tanveer Hussain2
...s, and single nucleotide polymorphisms (SNPs) were evaluated. The MEGA X-based maximum likelihood method was employed to study the genetic relatedness between sequenced samples and reference sequence as well as other deer. Among query sequences, the greatest genetic distance was observed between #B2_N1 and #H2_N7 i.e., 0.1184 and smallest genetic distance is seen between #C2_N2 and #G2_N6 i.e., 0.0210. The genetic distance between query samples and 25 other de...
Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*
...entify single nucleotide polymorphisms (SNPs) and genomic regions that are associated with ACMS in the Chinese Merino sheep population. To identify the genetic risk factors of alopecia in Chinese Merino sheep population, we carried out a genome-wide association study (GWAS). The 60 Chinese Merino sheep alopecia cases and the 190 Chinese Merino sheep controls were from the same livestock farm. DNA was extracted from ear tissue using the saturated phenol-chlorof...
Lingang Dai1,2,3, Xiang Chen1,2,3, Jiajin Huang1,2,3, Jiali Xu1,2,3, Meimei Xiao1,2,3, Jiajing Chen1,2,3 and Yong Ruan1,2,3*
...the effect of PGAM2 gene polymorphisms on the performance of F3 generation hybrid goats, the relationship between PGAM2 gene polymorphisms and slaughter traits, meat quality traits and organ coefficients was determined in 39 F3 generation hybrid goats. The results showed that the slaughtering performance of male goats was better than that of female goats, and there were highly significant (P<0.01) and significant (P<0....
UZMA PARVEEN1, MASROOR ELLAHI BABAR1*, NIDA BABAR2, TAHIRA MUGHAL3 & AKHTAR ALI1
...onship of G308A and -238 polymorphisms in Tumor necrosis factor alpha. We collected blood samples of 70 patients out of which 50 ladies gave untimely births and 20 ladies gave typical births. DNA extraction technique was applied. Primers were designed, amplified by PCR and RFLP were performed to identify the TNF–alpha -308 and -238 polymorphisms. The frequencies of TNF-alpha -308 genotype TNF GG, GA and AA were 37%, 10...
Tebogo Letsukulo Percy Thepa, Thobela Louis Tyasi*
...s. The single nucleotide polymorphisms (SNPs) of the MSTN gene have been implicated with growth parameters in a multitude of livestock species. The aim of the study was to conduct a comprehensive literature evaluation of the association between ovine growth traits and the SNPs of the MSTN gene. Four databases (PubMed, Web of Science, ScienceDirect and Google Scholar) were systematically evaluated using the keywords sheep, MSTN, polymorphism, genetic variation,...
Hadi Awad Hassooni1, Safaa Sabbar Atiyah2*, Alaa Saleh Jassim1
....05) between the genetic polymorphisms 180/170 and 190/181 in the California mastitis test. However, no statistically significant differences noticed in the genetic polymorphisms related to fat, protein, lactose, and solid non-fat in milk elements, with percentages of 3.60% and 3.48% for fat, 2.90% and 3.1% for protein, 4.18% and 4.31% for lactose, and 7.94% and 8.26% for solid non-fat for the two genetic
Tanveer Hussain1, Abdul Wajid1, Jabbar Khan2, Asif Nadeem1, Misbah Hussain1, Qurat ul-Ain1 and Masroor Babar1*
...ly sequenced to decipher polymorphisms. Only a single substitution (T→A) was found in non-coding region of IL-2 gene. Comparison of IL-2 gene sequence of all four breeds with other goat breeds showed high similarity in sequence. Phylogenetic analysis of our local breeds with other mammals showed that IL-2 was highly variable. This high substitution rate could be due to changed selective pressure. These rapid changes may also lead to the changes in the fun...
Sher Afgan1, Muhammad Asif2, Iqra Yaqoob3, Muhammad Latif4, Zureesha Sajid2, Kainat Akram3, Ghulam Mujtaba5, Manzoor Hussain6, Muhammad Farooq1, Mourad Ben Said7,8* and Furhan Iqbal3*
...sp; It is concluded that polymorphisms in GSTs may disturb the protection provided by them against oxidative stress which may influence disease progression in RA....
Zuyang Zhou1, Xi He2, Yufang Liu1, Qiuling Li3, Pingqing Wang2, Yongfu An4, Ran Di1, Yuze Yang5 and Mingxing Chu1*
...t, two single nucleotide polymorphisms (SNPs) were detected and genotyped in 407 Chinese Holstein cows. The non-synonymous g.16024G>A, g.17924G>A in KR and TE domains respectively resulted in a non-conservative substitution of alanine by threonine. g.17924G>A was associated with milk fat percentage, and the milk fat percentage of homozygous genotype AA was significantly higher than the other genotypes. The polymorphism information content (PIC) analys...
Uzma Zafar1*, Zaima Ali1, Ambreen Tauseef2 and Saba Khaliq3
...tion of adiponectin gene polymorphisms rs1501299 +276 G>T and rs266729 -11377 C>G with metabolic syndrome and to compare serum adiponectin levels in its polymorphic genotypes. It was an observational study conducted at University of Health Sciences, Lahore. Study approval was granted by institutional Review/Ethical Board. Informed consent was taken from all the participants. The venous blood sample was taken after an overnight fast of 8-10 h. Blood was s...
Ridha Tunnisa1, Muhammad Ihsan Andi Dagong2*, Sri Purwanti2, Sri Rachma Aprilita Bugiwati2, Wempie Pakiding2
... study aimed to identify polymorphisms of the (IGF-1) gene in Alope chickens and its relationship to the growth traits of Alope chickens. A total of 120 animals consisting of 52 cocks and 68 hens, were included in this study. Chickens were kept in individual cages to observe growth traits, including initial body weight, final body weight, daily body weight, body weight gain, feed consumption, and feed conversion ratio. Growth traits were analyzed using the gen...
Jennarong Kammongkun1, Doungnapa Promket2*
... (SL). Notably, NPY gene polymorphisms showed significant associations with growth performance and morphological traits (P<0.01). The Bb genotype was linked to higher body weight and average daily gain during weeks 4 to 16 compared to the bb genotype (P<0.01). This suggests an influence of the genotype on these traits. Additionally, NPY polymorphism affected body length (BL), with Bb genotype (21.10 cm) showed significantly higher BL values compared to t...
Nguyen Ba Trung1,2*, Pham Thi Kim Phuong1,2
Nguyen Ba Trung1,2*, Pham Thi Kim Phuong1,2
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