Population Density and Aggressiveness of Macrophomina Phaseolina Isolates from Sindh, Pakistan
Population Density and Aggressiveness of Macrophomina Phaseolina Isolates from Sindh, Pakistan
Khadim Hussain Wagan1*, Muhammad Ibrahim Khaskheli2, Jamal-U-Ddin Hajano1 and Abdul Ghani Lanjar2
ABSTRACT
Macrophomina phaseolina causing charcoal rot of sunflower is major fungal pathogen which can survive for months to years in soil and also over season in the seed. Therefore, population density and their aggressiveness to cause the disease is playing critical role for management of this pathogen. For such purpose colony forming units (cfu) per gram of soil and seed were determined and capability of previously characterized M. phaseolina isolates to cause the disease was tested on HO-1 variety. Additionally, most virulent isolate was characterized through sequence and phylogenetic analysis. There was significant variation (Df= 54, F=7.4, P=0.0000) among cfu per gram of M. phaseolina in soil samples collected during field survey at different districts of Sindh during 2015 and 2016 growing year. In soil sample maximum 41.371 cfu per gram of M. phaseolina with range of 27.00-48.40 were counted from Badin during 2016 followed by 40.2 with range of 27.00-45.20 during 2015 growing season. The plant tissues were carrying more M. phaseolina cfu as compared to soil samples. However, cfu count of M. phaseolina was significantly varying among districts, locations and year of samplings (Df= 54, F= 4, P= 0.0000). Similarly, as soil samples collected from Badin, plant tissue samples also gave maximum cfu per gram of M. phaseolina from Badin and then reduced gradually in Thatta, Tando M. Khan, Hyderabad, Shaheed Benazirabad, Mirpurkhas, Sanghar, Dadu, Sukkur and Khairpur. All the isolates could cause infection. Maximum plant mortality (46.67%) was recorded in plants inoculated with MPS16 isolate followed by MPS17 (43.33%) and MPS12 (43.0%). Similarly, length of necrotic lesions was also significantly higher in MPS16 (6.67 cm) followed by MPS17 (6.33 cm), MPS12 (6.30 cm), MPS15 (6.17 cm) and MPS 13 (6.07 cm). The predicted isolate (MPS16) was aligned with different species and observed that MPS16 belongs to M. phaseolina isolate. The sequence showed high identity with sequences with Macrophomina sp. from the NCBI database (100%); In contrast, alignments among the same genera and species demonstrated 91–98% identity. We then predicated that isolated encoded culture (MPS16) predetermine with Macrophomina sp.
To share on other social networks, click on any share button. What are these?
