Prospective Biochemical Analysis of Chronic Myeloid Leukemia Patients in Response to Tyrosine Kinase Inhibitors
Prospective Biochemical Analysis of Chronic Myeloid Leukemia Patients in Response to Tyrosine Kinase Inhibitors
Hafiz Muhammad Arsalan1, Amina Arif1 and Muhammad Khalil Ahmad Khan2*
ABSTRACT
Chronic myeloid leukemia (CML) is a chronic proliferating cancer of bone marrow presently treated with BCR-ABL tyrosine kinase inhibitors (TKI). Within interacellular system, increased reactive oxygen species (ROS) production in response to antioxidant (AOX) defense systems lead to oxidative stress (OS). Our study aimed at biochemical profiling of CML patients in response to imatinib or nilotinib therapeutic drugs. Fresh venous blood sample (10 mL) of 170 CML diagnosed patients and 10 healthy individuals was collected in heparin vial from oncology department, Mayo hospital and Jinnah hospital Lahore, Pakistan. Biochemical profiling of malondialdehyde (MDA), superoxide dismutase (SOD), catalase (CAT), glutathione (GSH), nitric oxide (NO), advanced oxidation protein product (AOPP), advanced glycation end products (AGE’s) and micronutrients (retinol, ascorbic acid, alpha tocopherol), complete blood count, liver profile, renal profile, lipid profile, serum electrolytes were evaluated in CML and control groups. Our study reported that no significant difference in biochemical profile among treated with Nilotinib or Imatinib tretated groups while in comparision to control group, a marked difference was observed. Antioxidant biomarkers i.e. MDA, SOD and CAT were augmented in control group as compared to CML treated groups. Decreased GSH level was reporetd in the CML group while increased in the nilotinib treated group compared to the Imatinib treated group. Other stress markers i.e. NO, AGEs and AOPP were also found to be high in level in control group compared to CML treated group. Micronutrient i.e. retinol, ascorbic acid, alpha tocopherol were increased in treated groups as compared to the control group. Our study concluded that oxidative stress that is responsible for the progression of CML is manageable with the use of chemotherapeutic drugs.
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