Proteolytic activity analysis in germination defective spores of Bacillus
Proteolytic activity analysis in germination defective spores of Bacillus
Uqba Mehmood
ABSTRACT
Proteolytic enzymes are characteristically produced by Bacillus species during sporulation and germination. The hydrolysis of spore proteins to free amino acids, is accomplished by proteases, in first 20 minutes of spore germination. The analysis of Spo mutants (strains which lack Sporulation (Spo) gene or have its inactive form) revealed that many of these strains did not produce extracellular proteases. Some evidence for the involvement of serine proteases in sporulation has been provided by inhibitor studies. Nevertheless variation in germination response of germination defective strains with different nutrients was recorded in ALA system, but overall responses of germination defective spores with substituted amino acids and alanine are not much different. Therefore all the amino acid proved competent germinant in place of alanine in ALA system. The overall role of germinants as a replacement in AGFK is satisfactory and they trigger germination in germination defective bacterial spores as do asparagines. In present studies all the germination defective spores showed very low proteolytic activity in comparison with proteolytic activity of normal spores of wild type Bacillus subtilis PY79. It appears that defective spores lack enzymes with proteolytic activity which is necessary for germination. Since all protease deficient mutants are asporogenous. Although some mutants, producing very low levels of protease are still able to sporulate, with some drawbacks.
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