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Screening and Characterization of Thermolabile Protease and Alkaliphilic Lipase Producing Psychrotrophic Stenotrophomonas sp. and Pseudomonas sp.

Screening and Characterization of Thermolabile Protease and Alkaliphilic Lipase Producing Psychrotrophic Stenotrophomonas sp. and Pseudomonas sp.

Yasir Ali1,2*, Bashir Ahmad1, Naqeebullah Jogezai3 and Adil Hussain

1Department of Biological Sciences, International Islamic University, 44000, Islamabad, Pakistan; 2Department of Chemical Engineering, Texas AandM University Texas 77840, USA; 3Department of Bioengineering and Space bioscience, Institute of Space Technology, 44000, Islamabad, Pakistan.

yasir.phdbt8@iiu.edu.pk  

Figure 1

E. coli 16S rRNA gene structure. 

Figure 2

TBA plate showing zone of hydrolysis of tested bacterial strains. A) Samples 1, 2, 4 and 5 are bacterial strains with lipolytic activity. Sample C is control one. B) Sample PAK-03 showing maximum zone of hydrolysis of Pseudomonas peli PAK-03. 

Figure 3

Phylogenetic tree of Stenotrophomonas sp., PAK-01 based on 16S rRNA sequence. The red tag shows position of Pseudomonas peli PAK-03 with other bacterial species. 

Figure 4

Phylogenetic tree of Pseudomonas peli PAK-03 based on 16S rRNA sequence. The red tag shows position of Pseudomonas peli PAK-03 with other bacterial species. 

Sarhad Journal of Agriculture

September

Vol.40, Iss. 3, Pages 680-1101

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