Study on the Treatment of Experimental Dermatitis with Ginkgo biloba Leaf Extract and Quercetin
Study on the Treatment of Experimental Dermatitis with Ginkgo biloba Leaf Extract and Quercetin
Minghui Xiao, Minjie Huang, Jie Dong and Deqian Wang*
ABSTRACT
Allergic contact dermatitis (ACD), is a classic delayed allergic reaction mediated by cellular immunity. P815 degranulation model was established by stimulating P815 cells with C48/80 inducer to explore the effect and mechanism of quercetin on mast cell degranulation. The ACD model of mice was established by 1-Chloro-2,4-dinitrobenzene (DNCB) external application to explore the therapeutic effect and mechanism of quercetin and Ginkgo biloba leaves extract on ACD. Quercetin could inhibit degranulation of P815 mast cells and significantly reduced the concentration of β-Hex, Histamine (HIS), IL-4 and TNF-α in the supernatant of degranulated cells (P<0.05), significantly decreased IL-4, TNF-α, Pik3r3, Akt2, Gsk-3β mRNA expression (P<0.05). G. biloba leaf extract and quercetin could significantly reduce the levels of IL-6, IL-4, IL-1β, TNF-α and IgE (P<0.05), the expression levels of TLR4, NF-κB1 and TNF-α were significantly decreased (P<0.05). Quercetin and G. biloba extract have good therapeutic effect on DNCB induced ACD mice.
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