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Thermodynamic Characterization of Kidney Superoxide Dismutase from Labeo rohita Exposed to the Mixture of Lead and Chromium

Thermodynamic Characterization of Kidney Superoxide Dismutase from Labeo rohita Exposed to the Mixture of Lead and Chromium

 Huma Naz1,*, Sajid Abdullah2, Khalid Abbas2 and Muhammad Anjum Zia3

 1Department of Zoology, Cholistan University of Veterinary and Animal Sciences, Bahawalpur

2Department of Zoology, Wildlife and Fisheries, University of Agriculture, Faisalabad
3Department of Chemistry and Biochemistry, University of Agriculture, Faisalabad

*      Corresponding author: [email protected]

 

Fig. 1.

Fractional summary of SOD by using ion-exchange column chromatography.

Fig. 2.

Effect of different pH (A) and temperature (B) on SOD activity.

Fig. 3.

 Effect of NBT concentration on SOD activity in the kidney of control (A) and stressed (B) L. rohita.

Fig. 4.

 Arrhenius plot for the determination of irreversible thermal denaturation of SOD from kidney of control (A) and Pb+Cr exposed (B) L. rohita.

Fig. 5.

Pseduo-first order plot of irreversible thermal inactivation of SOD from kidney of controlled (A) and Pb+Cr exposed (B) L. rohita.

Pakistan Journal of Zoology

October

Pakistan J. Zool., Vol. 56, Iss. 5, pp. 2001-2500

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