Transcriptome Analysis in the Fat Body of Two Silkworm (Bombyx mori) Strains with Different Susceptibility to Fenvalerate
Guo-dong Zhao1,2*, He-ying Qian1,2, Yi-ling Zhang1,2, Gang Li1, 2, Jian Tang1, 2 and An-ying Xu1,2*
1School of Biotechnology, Jiangsu University of Science and Technology, Zhenjiang, No. 2 Mengxi Road, Jiangsu 212018, China
2The Sericultural Research Institute, Chinese Academy of Agricultural Sciences, Zhenjiang, Jiangsu 212018, China.
Guo-dong Zhao, He-ying Qian and Yi-ling Zhang contributed equally to this work.
Fig. 1.
Scatter plot of differentially expressed genes. (A) Lan5; (B) Mysore. X and Y axis represents log10 transformed gene expression level, red color represents the up-regulated genes, blue color represents the down-regulated genes, gray color represents the non-DEGs.
Fig. 2.
GO classification of up-regulated and down-regulated genes. (A) Lan5; (B) Mysore. X axis represents GO term. Y axis represents the amount of up/down-regulated genes in a category.
Fig. 3.
Pathway classification of DEGs. (A) Lan5; (B) Mysore. X axis represents number of DEG. Y axis represents functional classification of KEGG. There are six branches for KEGG pathways: cellular processes, environmental information processing, genetic information processing, human disease (for animals only), metabolism and organismal systems.
Fig. 4.
Verification of transcriptomics results of DEGs by qPCR. (A) Lan5; (B) Mysore. The y-axis indicates the relative expression level of gene mRNA transcripts (fold change=log2 ratio). The x-axis indicates the selected genes. Vertical bars represent the mean±SE (n=3).