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Laila M. Fadda1, Nouf M. Al-Rasheed1, Iman H. Hasan1, Hanaa M. Ali2,3*, Nawal M. Al-Rasheed1,4, Musaed Al-Fayez5, Aly M. Ahmed5, Nada Almutlaq1, Nehal Qasem1 and Reem Khalaf1

...f macrosialin cluster of differentiation (CD68). APAP elevated of most of the previously measured parameters and decreased GSH, SOD, and total protein levels. Liver sections of H&E demonstrated liver injury characterized by centrilobular hepatocellular necrosis, CD68, and Bax expressions were also increased. Treatment with all the aforementioned antioxidants downregulated most of the elevated parameters compared to the APAP-treated group. Treatment with th...

Atta ur Rehman1, Sher Ali2 and Sajid Malik2*

...r to observe the genetic differentiation in lower Khyber Pakhtunkhwa (KPK) region, ABO and Rh blood groups record of nine Pashtun populations, i.e., Bannu, Dera Ismail Khan, Hangu, Karak, Kohat, Kurram Agency, Lakki Marwat, South Waziristan Agency, and Tank, was collected. Absolute gene diversity, gene differentiation and genetic diversity were estimated to be 0.01, 0.019 and 0.515, respectively. There were the highest affin...
Andrea Rezic1, Ivica Boskovic2, Piera Lubinu3, Marina Piria1, Tihomir Florijancic2, Massimo Scandura3 and Nikica Sprem1*
...ssion of form and better differentiation between the sexes.
...

 Nosheen Noor Elahi* and Misbah Mughal

...haracteristic of variety differentiation.

...
Tianzhu Chao, Huiqiang Cai, Yuxun Zhou, Kai Li* and Junhua Xiao
...tribution, diversity and differentiation of the house mouse Mus musculus provide insights into past dispersal events in natural populations of this species. We investigated the molecular phylogenetics of Chinese house mice based on 466 DNA sequences from mitochondrial D-loop fragments. Our analyses revealed that Mus musculus musculus and M. m. castaneus are older colonized subspecies with broad distributions in East China, while M. m. d...
Jun Gao1,2, Liang-Liang Yue3, Xianhuan Jiang1, Liju Ni4, Muhammad Aqeel Ashraf5, Yuxun Zhou,1 Kai Li1,* and Junhua Xiao1,*
...yed an important role in differentiation of M. fortis in China. Furthermore, the samples all from Southwest China in the GX group may be an example of ‘refuge within refugia’ in glacial period. According to our molecular clock analysis, the main clades of M. fortis divergence and separated time at around 0.77±0.64 million years ago (Mya) located in the Penultimate Glaciation. Divergences within the three clades taken place duri...

Nirbhay Kushwaha, Achuit K Singh, Brotati Chattopadhyay and Supriya Chakraborty

Recent advances in geminivirus detection and future perspectives
...en developed that permit differentiation of viral pathogens.These methods, initially based solely on identifying the distinct biological characteristics of different viruses, were soon supplemented with methods based on light or electron microscopy and serology and subsequently by Enzyme Linked Immunosorbant Assay (ELISA) and finally the use of molecular (nucleic acid-based) techniques have revolutionized the diagnosis of plant viruses. While the technologies ...
Song Li1,* and Zuojian Feng2
...elationships between the differentiation of P. albiventer and its environmental evolution in southwestern China are also provided.
...
Fang Wang, Yong-Fang Jia, Po Wang, Qi-Yan Du and Zhong-Jie Chang*

 

...ts of letrozole on gonad differentiation, 5dph fry of carp were exposed to different concentrations of LET. At 130 dph, sex ratio was recorded. The results showed that continuous exposure to LET induced the changes of sex ratio. High concentration of LET can promote male-biased sex ratio. In order to reveal the molecular mechanism by which LET caused sex reversal, juvenile carp (120dph) were exposed to 0, 5, 25, 125, and 625μg/L LET for 15 days, and the exp...
Lihong Qin1, Guoliang Zhang1, Chaojie Zhu1, Jian Wu1, Zhihui Zhao2,* and Yumin Zhao1,*
...late cell proliferation, differentiation, apoptosis, and involve in the development of sperm. Meanwhile, DUSP4, PDK4 and FKBP1B were also found to participate in testis development and spermatogenesis. In this study, online software and luciferase reporter gene system were used to predict and verify the target relationship between miR-122 and DUSP4/PDK4, miR-449a and FKBP1B. Thereafter, comparison analysis was done on ...
Haixia Liu, Yuwei Ye, Yang Li, Xiaolin Liu, Dongmei Xiong* and Lixin Wang
...rsity and strong genetic differentiation have been found in this study. The baseline population genetic information supplied in this study will be vital for the protection and restoration of this endangered species.
...
Sherin Reda Rouby
...ability of RP030 gene in differentiation of lumpy skin disease virus and sheep poxvirus in a single PCR assay without the necessity of post processing steps. 
...
Adel A. Basyouny Shahin,1,* Amira M. Metwally Mohamed2 and Abd El-Reheem A. El Shatter2

 

... ago).
...
Tanveer Hussain1,*, Masroor Ellahi Babar1, Marcos De Donato2, Abdul Wajid1, Asif Nadeem3, Zahoor Ahmad3, Waqas Ahmad Khan4, Sunday O. Peters5 and Ikhide G. Imumorin6
...PGMA tree showed a clear differentiation between taurine and indicine cattle, except mitochondrial taurine sequences in Lohani and Nari Master breeds. The within-breed estimates of divergence were very low in all breeds except for Nari Master (mixed-bred). The estimates of divergence among breeds were also low for most breed pairs, except for Nari Master and Dhanni. While the overall genetic divergence within the B. indicus or within B. taurus we...
Riaz Aziz Minhas1,*, Muhammad Nasim Khan1, Muhammad Siddique Awan1, Basharat Ahmad1, Syda Shaista Bibi1, Mohsin Hanif1 and Afsar Mian2
...8±0.003), genetic differentiation constants among populations (Gst: 0.153±0.025) and within populations (Rst: 0.847±0.025) were calculated. Gene flow (Nm: 3.246±0.448) and genetic similarity (97-98%) between populations was high. UPGMA based dendrogram identified five distinct geographic groups, and Mantel tests (Rxy=-0.008, P>0.05) suggested a non-significant relationship between genetic distance and geographic distance. ...
Wei Meng1,3, Tianyan Yang2,*, Yunguo Liu1, Mahmut Halik1 and Tianxiang Gao2
...ic distance and sequence differentiation suggested that great genetic variation existed within species and the sample from Kaidu River in South Xinjiang might be a cryptic species or subspecies of G. dybowskii. The phylogenetic analyses from 12 concatenated H-strand-encoding protein genes were conducted by Neighbor-Joining method to reveal the evolutionary relationships within subfamily Schizothoracinae. Three different grades of schizothoracine fishes ...
Ai Guo1,2,3, Jiaguang Xiao4, Binbin Shan4, Tianxiang Gao5 and Yongdong Zhou3,*
...xhibited obvious genetic differentiation between C. mystus N and C. mystus S (d=0.083). C. reynaldi was at the basal part of the trees, and showed obvious genetic differentiations with other Coilia species (d>0.19). Our results suggested that the north and south lineages of C. mystus could be genetically distinct as different species.
...
Yuan Li1, Liyan Zhang2, Karhoe Loh3, Ji Feng1, Xinqing Zheng1, Puqing Song1 and Longshan Lin1,*
... showed that the genetic differentiation was mainly among populations. According to the distribution of the haplotypes, an ancestral haplotype existed in both the Chinese population and the Malaysian population, further confirming that the Chinese and Malaysian P. minor populations originated from the same refuge in the South China Sea. A historical demographic analysis indicated that P. minor experienced a recent population expansion during the ...
Aiguo Zhou1,3,4, Shaolin Xie1,4, Zhenlu Wang1, Lanfen Fan1, Yanfeng Chen2, QiaoYe1, Fang Zeng1 and Jixing Zou1,*

 

Hamidullah1,*, Arshad Javid2, Basit Rasheed1,Jehan Zeb3, Abidullah1 and Muhammad Iftikhar Khan1

 Sohaib Afzaal1, Usman Hameed2, Nasir Ahmad3,*, Naeem Rashid3 and Muhammad Saleem Haider1

...quences and strain level differentiation was carried out by sugar fermentation tests. These strains were further analyzed for their ability to produce lactic acid. Overall, Leuconostoc mesenteroides was found as pre-dominant group (41.1% prevalence) among LAB strains in the isolated food samples. Some opportunistic pathogens were also isolated from these media. Among the tested strains maximum amount of lactic acid (26.457 mg/mL) was produced by the lac...
Bibi Nazia Murtaza1,2, Azhar Qayum3, Shamaila Inayat Nadeem1, Naif Awdh Al-Maliki4, Abdulaziz Alamri4 and Abdul Rauf Shakoori2,5,*
...nt role in regulation of differentiation and cell growth, RasGTPases behave as genetic switches. This transient process of GAP-mediated GTP hydrolysis becomes altered when the Kras gene is mutated. The most common Kras mutations are found in codon 12 and 13 and 61. Some other noncanonical mutations have been reported in codon 11, 14, 15, 17, 18, 19, 20, 22, 27, 30, 31, 117, 146 and 154. We aimed to demonstrate the conformational changes induced in two novel K ...

 Ping Zhang1,2, Yabin Pu1, Yu Zhang2, Jia Chen2, Kunfu Wang3, Qian Li3, Yujiao Sun3, Yuehui Ma1, Shuqing Jiao2,* and Weijun Guan1,*

...tion, identification and differentiation potential of Muscle-derived stem cells (MDSCs) from sheep skeletal muscle and provide an experimental evidence for clinical application. The skeletal muscle tissue were studied from fetal sheep, and the stepwise digestion by XI collagenase and trypsin was used to isolate MDSCs and were purified by differential attachment technique. Cell morphology were observed using an inverted microscope, and MDSCs proliferation patte...
Bingbing Gao1, Na Song1, Zhonglu Li2, Tianxiang Gao3 and Liqin Liu3,*
...cated weak or no genetic differentiation among different populations. Haplotype network and NJ phylogenetic trees revealed that there was no distinctly geographical distribution pattern among the haplotypes. Furthermore, the mismatch distribution of pairwise nucleotide and the negatively selective neutrality test suggested that N. mannusella might have experienced a recent population expansion about 29, 000 years ago. The population expansion and coasta...
Songhao Zhang1, Yiwen Gong1, Jian Xu1, Mou Hu2, Peng Xu3 and Yanliang Jiang1,2,4,*
...knowledge of the genetic differentiation between different common carp varieties caused by domestication, and accelerate the molecular selection of fish species with consumer-favored skin color and body shape.
...
Yan-Bin Zhu1, Wang-Dui Basang1, Zhan-Dui Pingcuo1, Yang-Ji Cidan1, Sang Luo1, Dun-Zhu Luosang1, Yang-La Dawa1 and Guang-Xin E2,3,* 
...f human yak phylogenetic differentiation as well as providing data support for understanding the evolution and migration of yak population in future studies.
...
Sajida Sabahat1, Asif Nadeem1,*, Maryam Javed1, Muhammad Yasir Zahoor1, Abu Saeed Hashmi1, Ghulam Yasein2 and Ghulam Abbas1 
...eproduction. It controls differentiation and maintenance of different function in various and specific tissues. The liver is the primary source of IGF-1 and considered as candidate genes for growth because they play a significant role in development and growth regulation. IGF-1 control protein metabolism and is extremely conserved region amongst species. IGF-1 have not been studied before in camel. The DNA samples of Marecha camel were collected from the Camel...
Peng Ren1, Xian-Qing Liu1, Chao-Wu Yang2,3, Hua-Rui Du2,3, Xiao-Song Jiang2,3 and Yi-Ping Liu1*
... immune gene, Myeloid differentiation primary response gene 88 (MyD88) can activate the nuclear factor-κB (NF-κB) pathway and regulate downstream gene expression. Single nucleotide polymorphisms (SNPs) in the coding regions (CDS) of the MyD88 gene have also been reported to be associated with inter-subject differences in responses to Salmonella Pullorum infection in chickens. However, whether the 3’-untranslated re...
Hong-mei Gao, Wen-long Su, Gui-bin Tao, Han-yang Li, Wen-zheng Cao and Zhi-dong Qiu*
...t affect the erythrocyte differentiation of mouse CD34+ cells. Then, we tested the geniposide indirectly affecting BM accessory cells. We conclude that geniposide maybe will control the erythropoiesis in vivo and reverse the BM microenvironmental signals, and this could provide an idea to attenuate anemia in chemotherapy.
...
Yunfang Tian, Zeqin Fu, Jiantong Feng, Yahong Guo, Yingying Ye*, Jiji Li and Baoying Guo
...alues and networks. This differentiation was conformed to the geographic of the sampling sites. However, within the groups, the gene flow of the samples was frequency and showed non-significantly divergence. The results provided insights that the Yangtze diluting water and the ocean currents system resulted in the reduction of gene exchange from north to south during the spawning period and expanded the genetic divergence of M. meretrix between north an...
Bo Gao1,2, Fengmei Yang3, Wei Chen2, Xiaojia Song4, Xiaobo Liu5 and Dongmin Li1*
...tween varying degrees of differentiation and/or lymphatic metastasis, as well as variations in negative and positive expression between survival and recurrence times, were statistically significant. The level of tumor markers at 6 months after operation was significantly lower than that before operation in recurrent/non-recurrent and MDR1 positive/MDR1 negative group. There was a significant correlation between MDR1 expression and tumor markers (CA125 and CA15...
Peimin Yang*, Zongyun Hu, Yixin Liu, Guanghai Jin and Lei Wang
...ated significant genetic differentiation among populations (Fst=0.3202, P<0.01) and 68.98% of the total variation was resulted from intra-population differentiation. Pairwise Fst statistics also confirmed the presence of significant differentiation between populations, although no significant differentiation ...
Qingzhan Ma1, Bo Wu1, Jianping Jiang2 and Zhaobin Song1,3*
...ity. The largest genetic differentiation (FST) among the eight populations was 0.233 (between POPB and POPD) and the lowest FST was 0.04 (between POPA and POPE). Furthermore, population structure analysis and UPGMA dendrogram suggested that there were close genetic relationships between POPA and POPE, POPA and POPG, and POPF and POPH, respectively. POPH showed serious inbreeding based on Fis analysis.
Ming-Yan Shi2, Xu-Liang Yan1, Ping-Ping Han1, Yu-Jie Tang1 and Hui-Xia Li1*
...tion of multipotent cell differentiation and is essential for the regulation of adipogenesis. However, the differentially-expressed microRNAs (miRNAs) involved in SMSC adipogenesis induction, and the role of these miRNAs in Wnt/β-catenin signaling, has received little attention, particularly in livestock. In the present study, bovine skeletal muscle satellite cells (bSMSCs) were cultured and the Wnt/β-catenin signaling pathway was activated by LiCl. ...
Yahui Wang1, Ling Ren1, Yishen Xing1, Xin Hu1, 2, Qian Li1, Lingyang Xu1, Junya Li1* and Lupei Zhang1*
...ptimize the in vitro IMF differentiation model, the effects of bone morphogenic protein 4 (BMP4) and rosiglitazone during differentiation were studied. Comparing with control group, progenitor cells treated with BMP4 or rosiglitazone accumulated more intracellular lipid. Furthermore, the mRNA expression level of adipocyte-specific genes also increased significantly in BMP4 or rosiglitazone treated cells. The result indicated...
Huan Yang1, Tingting Li1, Huimin Zhao1,2, Xiaoyuan Zhang1, Huiyan Xu1, Yangqing Lu1, Xingwei Liang1, Shengsheng Lu1, Xiaogan Yang1*and Kehuan Lu1*
...ble of proliferation and differentiation, ensuring the efficiency of passing transgenes from parents to subsequent generations. In this research, to understand the growth characteristics of buffalo SSCs in vitro, methods for their isolation, enrichment, culture and preliminary identification were established. Testes from 3- to 6-month-old buffalos were digested with two-step enzymatic treatment to obtain isolated single cells and then enriched with a di...
Sara Sultan Alomran1, Muhammad Nasir Khan Khattak1,2, Amir Ali Khan1,2*, Sallam Hasan Abdallah2, Abeer Maher Fayyad1 and Khalid Bajou1,2
...coding RNA, on adipocyte differentiation from telomerase-transformed Mesenchymal Stromal Cells (iMSC3). The transfection of iMSC3 with miR-22-3p suppressed adipocyte differentiation as evident by reduction in lipid content and number of lipid droplets. While AKT3 is reported to be a target of miR-22-3p, our study indicated otherwise. Moreover, while miR-22-3p is known to target more than 500 genes, MAPK has not been identifi...
J.Y. Bai1,*, R.T.D. Wu2,*, Q. Zhang2, D. Bao2, L.M. Dao2 and X.H. Tian3
...ic polymorphism, genetic differentiation, gene flow and genetic distance of Alashan desert Bactrian camel, Alashan Gobi Bactrian camel, Sunite Bactrian camel and Qinghai Bactrian camel.The results indicated that the number of effective alleles of the four Bactrian camel varieties ranged from 2.7302 to 3.0524, and average theoretical heterozygosity and average polymorphic information content were 0.6283 and 0.5546, respectively. Observational heterozygosity, ex...
Hesham M. Abd El Halim1, Maysa M. Hegazy1,2, Aishah Alatawi3, Imtiaz Ali Khan4 and Munawar Saleem Ahmad5*
...e vital for starting the differentiation program of cells at discrete steps during development are major targets for biocide plant extracts. The current study evaluates the in vitro toxic effects of golden piller (Cupressus macrocarpa) and galangal (Alpinia officinarum) against Spodoptera frugiperda Sf9 cell line. Also, changes in gene expression levels of chitinase genes (SFch-1, SFch-2) and fibroblast growth factor receptor gene (SFbtl) ...
Zeynep Sonmez1 and Memis Ozdemir2,*
...s in GDF5 (growth differentiation factor 5 (CDMP-1)) and EPS8 (epidermal growth factor receptor substrate 8). After the PCR analysis of the identified gene regions of 72 head Holstein cattle, samples were separated by the high-resolution melting analysis (HRMA), and sequence analysis was applied to some randomly selected samples. New polymorphisms were identified in the determined genes and gene regions as a result of the sequence analysis...
Xiangzu Wang,1,2 Jifeng Xi,1,2 Bin Jia,1,* Yongsheng Zhang,1 Chaocheng Li1
...rowth, proliferation and differentiation in tissue cells while the Bcl-2 and BAX genes regulate apoptosis. This experiment adopted the real-time fluorescence quantitative technique to test ZFX, Bcl-2, and BAX expression in mice kidney tissue on 1 d, 7 d, 14 d, 21 d, 42 d, and 90 d after birth. The results showed that with the maturation of mouse kidney, the cell proliferation activity decreased, and the expression of ZFX, Bcl-2, and BAX were down regulated. Th...

Magdy, Mariam1 ; Abou ElHassan D.G. 2 ; and Salem, S.A.H 3

...se competitive ELISA for differentiation between natural
infected and vaccinated animals by detection of Non-Structral protein of FMDV by priocheck
test. Five hundred sera were collected from vaccinated cattle and buffalo from five Egyptian
governorates (Two governorates represent Delta region: El-Gharbia – Kafr El Sheikh , Two
governorates represent upper Egypt: El-Fayoum – El-Menya , and one governo...
Ashraf S. Khameis, lamya F. Atteya, Ayman H. Mansour, Heba A. Abdelhady, Ashraf A.
Saad
...div>characterization and differentiation of Capripox viruses. This finding may provide new information on
the epidemiology of SPV in El Menofiya governorate.
...
Sen Liu1,2, Yue Zhu2, Haixia Leng2, Ying Wang1, Xiangju Yin1 and Yajun Zhao1*
...n tissue development and differentiation as it plays an important role in gene expression regulation. In previous studies, epigenetic variation in the muscles of bat populations were explored; however, the extent of the patterns and levels of genomic DNA methylation among tissues in chiropteran animals have yet to be investigated. In this study, the epigenetic variation of six tissues in normal state Rhinolophus ferrumequinum (i.e., no pregnancy, nursin...
Gong Xue-na1, Jia Ting2, Zhang Di2 and Zhu Wanlong1*
...s regulation. Phenotypic differentiations in E. miletus of two regions may be closely related to food resources, altitude and temperature of Hengduan mountain regions.
...
He Yujiao1,2, Xie Huichun1, Lin Gonghua2,3, ZhangTongzuo2,3, Su Jianping2,3 and Du Yurong1*
...ulation patterns and the differentiation time in the plateau pika using mitochondrial DNA (mtDNA). Analysis suggests that the differentiation within this species could be related to quaternary glaciation. We also compared the clustering analysis results generated using mtDNA versus microsatellite markers. The most obvious difference between the two markers was reflected in the fact that the populations Yelashan (YLS) and Ban...

Jiao Ma*

...or self-regeneration and differentiation into cell types that can play an important role in embryogenesis and embryonic development. So far, seven types of cardiac stem cells with different molecular phenotypes and differentiation potentials have been discovered and studied. Increased proliferation and differentiation of these cells in areas of cardiac ischemia is a significant factor in t...

Yao Zou, Shien Ren, Miao Xu, Nannan Liang, Xuxin Zhang, Chongxuan Han and Xiaoning Nan

...okors, the interspecific differentiation of their skulls is still unclear. To differentiate among species and to describe the sexual dimorphism within each species, we measured morphological variation using one-way analysis of variance and cluster analysis in four species of zokor including Eospalax cansus, Eospalax rothschildi, Eospalax baileyi, and Myospalax aspalax. We also used principal component analysis and dichotomy to explore key measurements which co...

Jing Fu

...athological type, tissue differentiation, prognostic outcome, disease course and clinical stage (P<0.05). According to immunohistochemical examination, the co-expression of SATB2 and CK20 could improve the specificity of mucinous ovarian tumors. In addition, SATB2/CK20 co-expression is related to recurrence, pathological type, tissue differentiation, prognostic outcome, disease course and clinical stage.

...

Lifang Zhang, Xiuyue Zhang and Bisong Yue*

... higher level of genetic differentiation was detected among populations with mean Fst was 0.0891, the mean Nm was 2.5573, the genetic identity ranged from 0.4224 to 0.9405, the genetic distance ranged from 0.0613 to 0.8617. UPGMA cluster analysis indicated that the 12 populations were grouped into three major clusters, which showed that there had rich genetic diversity in P. americana, and the genetic differentiation to some...

Maskur Maskur1*, Muhammad Muhsinin1, Lestari, Tapaul Rozi1, Asnawi2 

...the value of the genetic differentiation index (Fst) in all populations is zero (0), indicating that there is no differentiation between breeds at the observed loci.

Keywords | Local duck, Microsatellites, Morphometric, Inbreeding and differentiation 

...

 Hanan Fathy Hammouda1, Mohammad Mahmoud Farag2*, Mervet M.F. El Deftar3, Mohamed Abdel-Gabbar4, Basant M. Mohamed4

...o confirm the osteogenic differentiation process. Also, cell viability assay by MTT was used to measure the number of viable osteoblast cells cultured with scaffolds extracts. Also, the antitumor activity of the scaffolds was studied against cancer osteosarcoma cell lines using Sulforhodamine B (SRB) assay. The results showed that addition of cerium-doped nanobioactive glass to the composite scaffolds was triggered an increase in cell growth, prolife...

Shahid Sherzada1,2*, Muhammad Naeem Khan1 and Masroor Ellahi Babar2

...volution and low genetic differentiation among populations of C. mrigala.

...

Zaniar A. Abas1, Mohammed Omer Baba Sheikh2,5*, Hardi N. Aziz3, Omed I. Abid4 

...n in Iraq.  Genetic differentiation study based on the genetic variation on the VP2 gene has shown that (CPV-2/Sul) strain belongs to new CPV-2b and CPV-2c, however, the (CPV-2/Erbil & CPV-2/krk) strains belong to new CPV-2a. A Phylogenetic tree constructed basis on the VP2 gene revealed that the field virus sequences from the present study were divided into two main phylogenetic groups (cluster A and cluster B), 10 new CPV-2a from a field isolate bel...

Ghassan Zahid1, 2*, Sara Iftikhar3, Muhammad Umer Farooq4 and Shakeel Ahmed Soomro5

...ease diagnostics and sex differentiation by administering the powerful diagnostic polymorphism detection tools at specific loci and the genome level. This review focuses on exploring the application, recent advancements and future implications of DNA-based molecular markers with the context of their role in the genetic improvement of fruit germplasm that is widely grown in Pakistan like citrus, apples, mangoes, guavas, bananas, date palms and peaches, etc. Fur...

A. El-Shemy1*, Hoda M. Mekky2, M. A. Bosila2, A. M. Allam1, Kh. M. Elbayoumi2, M. M. Amer3 

...vealed the occurrence of differentiation between our field DHAV-1 isolates and the vaccine strain. Therefore, applying an appropriate vaccination program for breeder duck flocks is significant in controlling DHAV-1 infection. Thus, we recommend the application of further studies to detect the efficacy of the used vaccine against the current field strains of DHAV-1

Keywords | Nervous signs, DHAV-1, RT-PCR, Nucleotide sequences, Phylogenetic analysis&nb...

Febriza Dwiranti1, Nur Fadhilah2, Ursula Paulawati Maker2, Priyo Sambodo3* 

...let count, and leukocyte differentiation by laboratory analysis. Descriptive morphological data and quantitative data were analyzed using ANOVA testing. The adult cuscus appeared to have more spots (the male spotted pattern was larger than that of the female) than the juvenile cuscus, where the body pattern had not formed spots (spread). Based on such an obvious spot pattern, the sexes of S. papuensis could be distinguished by males having larger spots than fe...

Caiyun Ma1, Hebao Wen1, Lu Li2, Gaofeng Liu1, Chunjing Wang1, Yu Guo1, Shiji Fang1, Hongyu Wang1* and Changqing Liu1*

...iological properties and differentiation multipotency of CSPCs. CSPCs were characterized by the clonogenicity, growth curves and karyotype analysis, respectively. The results showed that the CSPCs of Tibetan mastiff displayed self-renewal ability, proliferative potential, and hereditary stability. Moreover, the CSPCs of Tibetan mastiff also positively expressed recognized surface antigens of human CSPCs. Additionally, CSPCs exhibited d...

Xibin Liu1,2,3, Weijun Guan2,* and Dong Zheng1,*

...at they have an in vitro differentiation ability. These findings provide a theoretical and experimental basis for cell transplantation therapy in tissue engineering.

...

Masixole Maswana, Thinawanga Joseph Mugwabana and Thobela Louis Tyasi*

Qingyue Han1, Ying Li1, Jichang Deng1, Kunxuan Huang1, Yanyang Yang1, Quanwei Li1, Zhuowei Zhang1, Na Qiao1, Yanju Ji2, Khalid Mehmood3, Sarfaraz Ali Fazlani4, Hui Zhang1 and Zhaoxin Tang1*

...development of heart and differentiation of myocardial fibers in neonatal piglets by changing the expression of the IGF pathway and myocardial cells proliferation.

...

Jinzhao He1, Pengfei Feng2, Junqi Qin2, Yun Teng1, Xu Luo2*, Zigui Chen1*, Huawei Ma2* and Dayan Zhou1

... mechanism of body color differentiation and variation of red tilapia, selecting the main genes related to the variation and cultivating the pure and stable red tilapia variety. The effects of different temperature treatments on body color and survival of Guam red tilapia, pearl white red tilapia and Florida red tilapia were compared. Besides, comparative transcriptome analysis was used to screen the candidate genes linked to the skin color

He-Cai Zhang, Tian-Ge Hu, Chang-Ying Shi, Guang-Wen Chen* and De-Zeng Liu

...ggested that the genetic differentiation of D. japonica in Taihang Mountains was very great (FST=0.405, P<0.01). This significant genetic variation mainly occurred within populations (59.52%), followed by 36.54% deriving from among populations. As for demographic analysis, positive Tajima’s D and Fu’s Fs values (0.250 and 1.657, respectively) were found in concatenated Cytb/ITS-1 sequences as a whole for neutrality test. Moreover, the mismatch d...

Mohamed M. Bahr1, Mohamed S. Amer1*, Khaled Abo-EL-Sooud2, Shaimaa M. Kamel3, Marwa A. Fouly4, Ahmed N. Abdallah5, Ashraf A. Shamaa1, Omar S. El-Tookhy1 

...sion, proliferation, and differentiation of the recipient cells. Methodology: 24 male New Zealand albino rabbits were used in this study, divided randomly into 4 groups (6 rabbits per group), induction of corneal ulcers centrally in groups (I, II) and peripherally in (III, IV). Treated groups are (I, III) using topical lyophilized canine Mesenchymal Stem Cells derived EVs & control groups are (II, IV) using topical normal saline. Result: both treated group...

Jie Chen, Qinghua Qiu, Yanjiao Li, Xianghui Zhao, Lanjiao Xu, Xiaowen Xiong, Qingqi Wen, Mingren Qu and Kehui Ouyang*

...on autophagy, adipogenic differentiation and fat metabolism between subcutaneous and visceral pre-adipocytes. C57BL/6J male mouse primary subcutaneous and visceral pre-adipocytes were isolated and in-vitro adipogenic differentiation was performed in high-glucose medium (4500 mg/L glucose, HGM) or low-glucose medium (1000 mg/L glucose, LGM). Lipid contents in adipocytes were determined by oil red-O staining. Autophagy, adipog...
Ayesha Ahsan1, Rabia Arif2*, Samina Nazir1, Muhammad Saleem2 and Memunna G. Shahid1
...th, development and cell differentiation through phosphorylation and de-phosphorylation of other proteins. In this study, we have amplified PKC and beta tubulin homolog bml gene from six strains and F3 and F4 generations of Sordaria fimicola collected from the Evolution Canyon-1. Sequenced products of 464 bp of tubulin gene and 548 bp for PKC gene were aligned to observe the genetic variations between the eight parental strains of S. fimicola and reference seq...

Desi Kurniati Agustina1,3, Suyadi Suyadi2*, Veronica Margaretha Ani Nugiartiningsih2, Kuswati Kuswati2 

...l profile, and leukocyte differentiation of Madura cattle and its crossbreeds of different sex and age. The results are expected to provide a better understanding of the condition of livestock raised by the community. Blood samples were obtained from 60 heads of Madura and Limura (Limousine x Madura) cattle, which were differentiated by sex and age. Blood samples were collected into tubes and immediately stored in a cold box at approximately 4°C. Data anal...

Qi Zhuo, Yuanchun Yao, Meisongzhu Yang* and Jinhua Chen and Miao Tian

...normal proliferation and differentiation of cancer cells.

...

Qi Liu1,2, Weiyuan Li2,3, Qi Zhang2,3, Ziwei Wang2,3, Rui Gao1,2, Wenlei Liu1,2, Lei Zhang1,2, Ying Liu1,2, Tao Tian2,3 and Hongwei Yan2,3*

... but significant genetic differentiation was detected between the 2019 stock and the stocks of the previous two years. Given the continuously large scale of artificial enhancements in this area, these data may indicate that releases of hatchery-reared F. chinensis individuals may be associated with inbreeding and potentially the reduction in genetic diversity of the F. chinensis population.

...

Yingying Ye1, Chengrui Yan1, Kaida Xu2, Jiji Li1, Jing Miao1, Zhenming Lü1 and Baoying Guo1*

...tructure with an average differentiation estimated between populations of 0.014. Knowledge obtained from this study has important implications for the management of existing genetic resources for aquaculture, artificial releasing project and conservation in cuttlefish.

...

Bing Yan1, Meng Meng Zhang2, Xi Chen2, Yongming Li2*, Muhammad Khan3* and Tonghui Ma1*

...ial of self-renewal, and differentiation into pathogenomic heterogeneous tumor formation leading to therapeutic resistance. Therefore, targeting CSCs and their associated signaling pathways is crucial for effective treatment of GBM. In this study, we showed that alantolactone (ALT) hold the capacity to suppress stem-cell-like phenotype (SCLP) of glioblastoma cells. ALT inhibited the growth, reduced mammosphere formation and down-regulated the expression of CSC...
Sri Rahayu*, Muhaimin Rifa’i and Widodo 
...he antral stages. Growth differentiation factor 9 (GDF9), which is secreted by oocytes, is a paracrine factor that plays a vital role in follicular development. We performed a computational biology analysis to determine the role of GFD9 in mammalian ovarian function. We constructed a three-dimensional model of GDF9 using I-TASSER and human GDF9 protein sequences obtained from UniProt. We also analyzed GDF9 gene expression levels using GENEVESTIGATOR. Furthermo...
Rabail Hassan Toor1,3, Raazia Tasadduq2, Jane B. Lian3, Janet L. Stein3, Gary S. Stein3 and Abdul Rauf Shakoori1*

Di Zhou1, Qingmeng Long1*, Rong Yang1, Xiaoshan Tan1, Jun Li1, Mingyan Tang1, Zhonghai Zhao3, Ye Ao2, Zhinan Zhou1 and Changxue Chen1

...DEGs were primarily cell differentiation, reproduction and biological regulation. The associated gene functions were related to organelle, cell membrane and cell structure in cell component processes and for molecular function, the genes were related to protein binding, catalytic activity and cell proliferation. Moreover, these DEGs were enriched in the categories of signal transduction, reproductive system, endocrine system and cancer-related pathways and lin...
Guo-Hai Wang1, Ji-Feng Long2, Li-Juan Wei3, Zhi Qin2, Wei Yao4, Chuang-Bin-Tang1* and Qi-Hai Zhou4*
...g the mechanism of niche differentiation among sympatric species and the coexistence of such species communities. However, there is no clarity on whether the activity patterns of the sympatric species red-bellied squirrels (Callosciurus erythraeus) and Northern tree shrews (Tupaia belangeri) overlap; knowledge on the coexistence mechanism of these animals in the karst habitat is also minimal. Herein, we used camera traps to collect photos and research the acti...

Faiza Naeem1, Muhammad Farooq Sabar1, Muhammad Usman Ghani2,*, Qurat Ul Ain1 and Qurat-ul-Ain Zafar1

...and (hsa04659) Th17 cell differentiation pathways having STAT6, IL4, and IL13 genes having contribution in asthma. A network of appropriately matched proteins was provided by the string that was further utilized for centrality-measurement which ranked the thirteen most persuasive genes from top to bottom as IL10, IL4, ORMDL3, IL13, MS4A2, ADAM33, STAT6, GSDMA, IKZF3, LRRC3C, IL27, CD14, and ACE. This study enlightens the significantly associated asthma genes i...

Shudong Liu1,2, Shuai Wang1,2, Haoran Cui1 and Genyuan Chen1,2*

...e involved in derma cell differentiation, diet-induced obesity, and nervous system development. The genomic regions identified in this study provided a start-up point for contribute to similar studies and can facilitate the potential utilization of genes involved in etiology of Chinese Merino sheep alopecia in the future.

...
El-Dougdoug, Kh.A. t , S.A. Ghaza12 , A.A. Mousa2, H. Fahmyj and A.R. sofy2 

Afrasyab Khan1,3, Ali Raza Jahejo1,3, Meng-li Qiao1,3, Xin-yu Han1,3, Raza Ali Mangi1,3, Ding Zhang1,3, Yu-hai Bi2, George F Gao1,3* and Wen-xia Tian1,3*

...ession of MyD88 (Myeloid differentiation primary response 88), CCL5 (C-C Motif Chemokine Ligand 5), MDA5 (melanoma differentiation-associated protein 5) IKBKE (inhibitor of nuclear factor kappa-B kinase subunit epsilon), NFKBIA (NF-kappa-B inhibitor alpha), NFKBIE (NF-kappa-B inhibitor epsilon), Interferon Alpha (IFN-α), cMGF (chicken myelomonocytic growth factor), and TRAF6 (Tumor necrosis factor receptor-associated f...

Laila Ibouzine-Dine1*, Inssaf Berkiks1,2, Mouloud Lamtai1*, Hasnaa Mallouk1, Ayoub Rezqaoui1, Abdeljabbar Nassiri1, Abdelhalem Mesfioui1, Aboubaker El Hessni1

...ng neuronal survival and differentiation, neurogenesis, synaptogenesis, pruning, and brain plasticity. In the present study, we aimed to determine the activity of NO during the early and late critical periods of structural and functional maturation of the central nervous system (CNS). In this study, we focused on the prefrontal cortex, hippocampus, striatum, and hypothalamus of male and female “Wistar” rat pups, as these areas have differential dev...

Xin Niu1, Xuelan Fang2, Sang Ba3, Yihao Fang1,4, Davide Fornacca1,7, Kun Tan1,4, Yanpeng Li1,4,5*, Zhipang Huang1,4,5* and Wen Xiao1,4,5,6,7

...stify;">Ecological niche differentiation among sympatric animal species is a primary mechanism maintaining a long-term and stable coexistence. Information on the pattern of coexistence among sympatric species is vital to provide specific conservation measures and management. This study focuses on the spatial-temporal niche differentiation strategy adopted by black-and-white snub-nosed monkeys (Rhinopithecus bieti) and rhesus...

Tanveer Jilani1*, Irfan Khan2, Asmat Salim2, Fareena Bilwani1, Bushra Moiz3 and Mohammad Perwaiz Iqbal1,4

... blood cells. Cluster of differentiation (CD)34 is a known surface marker for identification of primitive human erythroid progenitor cells including EPCs. CD34+ cells can be isolated from peripheral blood samples using magnetic-assisted cell sorting (MACS) or by flowcytometry-mediated cell sorting. There are no reported studies from South Asia in which CD34+-derived EPCs have been isolated and purified from the peripheral blood samples obtained from healthy ad...

Chengrui Yan1, Jing Miao1, Jiantong Feng1, Liping Xia1, Yingying Ye1,2*, Jiji Li1 and Baoying Guo1,2

...l branch and indicated undifferentiation of T. clavigera in the China Sea. Migration dynamic analysis showed that gene flow was asymmetrical and QD as the source population. Additional, canonical correspondence analysis (CCA) analysis between environmental factors (SKT, TOP, SLP, and SUND) and haplotypes indicated that SLP and SUND carried highest influence on the haplotype distribution. SKT and total precipitation TOP were highly correlated with haplotype for...
Shams-ur-Rehman
...ds. An ecotypic level of differentiation has been confirmed with several recommendations to improve plantations of the species in northern Pakistan and other countries....
Rehana Perveen, Mehmood Akram and Ihsan Ilahi
...d on further growth and differentiation of the callus. At NAA 1 mg/1, BAP 0.5 mg/1 NAA 0.5 mg/1 roots developed at 0.5mg/1 of BAP & NAA 0.5+1.0 mg/1 of BAP shoot buds also developed. Callus pieces cultured on MS medium supplemented with different combinations of NAA &BAP showed progressive anatomical changes accompanying differentiation of roots and shoot buds....
K.M. Siddiqui and M. Saleem Khattak
...ough to promote genetic differentiation (Wright, 1953, 1962; Strand, 1967; Sluder, 1969). On the other hand, though it implies that breeding unit is small in most tree species, even in those species with arealy continuous range, still isolation is slight in most tree population. Environmental and physiological factors may also influence pollen movement within and between populations....

Hong Chen1,2, Jiawei Liu1,2, Chuan Lin1,2, Hao Lv1,2, Jiyun Zhang1,2, Xiaodong Jia1,2, Qinghua Gao1,2 and Chunmei Han1,2*

...es the proliferation and differentiation of antler chondrocytes, osteoblasts, and T lymphocytes through biological signals such as WNT, IHH, and IL2RA to ensure the development of antler tissue again after top pruning. To sum up, our data implied that BVES gene regulated mesenchymal stem cells’ proliferation and differentiation activities in response to ischemic stimuli to promote the healing and rapid growth of wounde...
Hong Su1,2, Lu Chen1,3, Wenrui Guo1, Daqing Wang1,3, Aolei Dou1,2, Jie Su1, Yanyan Yang4, Ying Tian4, Tingyi He4, Caiyun Wang1, Chenguang Du1, Haijun Li1, Xihe Li5, Guifang Cao1*, Yongli Song5* and Fuxiang Bao3*
... absorption and cellular differentiation, such as DPP4, ACE2, PLPP1, CD22, CD44, TEC, and JAK1. In the pathway analysis, the development of Hulunbuir short-tailed sheep 16-day embryo H(E16) highlighted pathways primarily associated with cellular interactions and organogenesis. These pathways are critical for transcriptional control during early embryonic development, especially organ formation. Genes that sophisticate organs and body mechanisms, and genes asso...

Shumaila Usman1,2, Irfan Khan2, Kanwal Haneef3 and Asmat Salim2*

... way to enhance the transdifferentiation ability of mature fibroblasts by using transgene free cellular approach in order to improve the cell-based therapeutics for diabetes. In the present study, we analyzed the effect of 2, 4 - Dinitrophenol (DNP) on the transdifferentiation of NIH3T3 cells into insulin producing β-cells (IPCs). DNP is a lipophilic weak acid that uncouple oxidative phosphorylation by decreasing ATP pr...

Rongrong Wang1,2, Da Ji1,2, Junjie Yao1,2*, Wenzheng Zhang1,2, Xianjun Zhou1,2, Xin Su1,2 and Tianquan Bai3

...ic diversity and genetic differentiation status of Craspedacusta sowerbyi Lankester, 1880 in Guizhou Province, genetic diversity and genetic structure analyses of three geographical populations of C. sowerbyi in Guizhou Province were performed using the results of PCR amplification and direct sequencing based on the mitochondrial DNA COI gene. Sixty-two individuals displayed four haplotypes, and the overall haplotype diversity (Hd) and nucleotide diversity (Pi...

Pangesti Nugrahani1*, Hery Purnobasuki2, Arif Nur Muhammad Ansori3, Jatuporn Anuchai4 and Anugerah Dany Priyanto5,6

...lating cell division and differentiation. This study aimed to assess the impact of BAP on various growth parameters, including shoot initiation, root initiation, leaf initiation, shoot and root length, plantlet weight, root number, leaf number, and shoot number. The study followed a completely randomized design with one factor, namely the composition of the planting medium, consisting of five treatments: full MS without BAP (MS0), ½ MS without BAP (&fra...

Bushra Sial1, Arif Muhammad Khan1, Mohsan Raza1,6*, Azhar Abbas Khan2, Sayyad Ali Raza Bukhari1, Muhammad Kashif Hanif3, Sher Khan Panhwar4, Muhammad Ashfaq5

...indicating a significant differentiation between populations of Taiwan and Pakistan. In contrast, the lowest Fst value of 0.04 manifested minimal genetic differentiation between populations in the USA and Bangladesh. An average genetic distance using the Kimura 2 parameter (K2P) model using BOLD systems revealed 20.17 and 19.87 percent within genus and family respectively. Nevertheless, this study documented the COI sequence...

Ali Mobder Niyf*, Jafar Ghazi Abbas Al-Jebori 

...tive tissue layer due to differentiation of the most mesenchyme cells to smooth muscle fiber (muscularis interna and muscularis externa). Taken together, the present study showed that the large intestine in local awassi sheep fetuses in the first trimester of gestation was located relatively in the middle part of the abdominal cavity and covered completely by huge liver and where it started to move gradually to the caudal aspect at second trimester, behind the...

Journal of Animal Health and Production

November

Vol. 12, Sp. Iss. 1

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