Youssef Lhor1*, Mounir Khayli1, Mohamed Bouslikhane2, Mehdi El Harrak3, Ouafaa Fassi Fihri2
Email: [email protected]
...2009. Interestingly, the infectivity rate of BTV was also higher during these periods indicating that high risk of infection is directly proportional to that of transmission vectors. Moreover, results demonstrate that the life stage “bumper” of the female imicola is the indicator of the infecting activity of C. imicola. Collectively, the distribution patterns and emergence of BT can be predicted by the abundance of the Culicoide...
Ghulam Ali Bajwa1, Faizah Abood2, Yusof Bin Ibrahim3
...perature and B. bassiana infectivity was analysed. Beauveria bassiana infection was in range of 27-30ºC. Based on these findings, it is concluded that B. bassiana has promising potential as microbial control agent against A. sciodoxa and should be exploited under field conditions....
Hira Akhter1, Bilal Aslam2*, Naveed Shahzad3, Tanzeela Farooq4, Muhammad Umer5 and Muhammad Hidayat Rasool2
...ers of 5.37 revealed the infectivity of the flock with H9N2 Influenza virus. To investigate the presence of virus in the study population, trachea, lung and intestine tissue samples were processed for RNA isolation and subjected to molecular detection of H9 gene of H9N2 subtypes using one step Reverse Transcriptase Polymerase Chain Reaction (RT-PCR). Out of 24 samples taken from each organ, 22, 21, and 18 samples from trachea, lungs, and intestine showed viral...
Shams Ur Rehman, Muhammad Arif and Abdul Mateen
Soil-borne viruses in major potato growing areas of Pakistan
...s expression, bait test, infectivity assays and double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Both the viruses were prevalent in Hazara and Malakand divisions. The average symptoms based incidence in Hazara divi-sion for PMTV was 8.83% and TRV was 6.80% while in Malakand division the average symptoms based inci-dence for PMTV was 8.46% and TRV was 9.95%. DAS-ELISA based average incidence in Hazara of PMTV and TRV was 17.34% and 11.99%...
Ali Murad Rahoo,1,2,* Tariq Mukhtar,1,3 Shoukat Ibrahim Abro,4 Barkat Ali Bughio5 and Rehana Kanwal Rahoo6
...fected by their level of infectivity and reproductive capacity. Therefore, in the present study the productivity of five entomopathogenic nematodes (Steinernema feltiae, S. kraussei, S. carpocapsae, Heterorhabditis bacteriophora and H. indica) were compared in Galleria mellonella larvae. The production of infective juveniles (IJ) in G. mellonella was significantly affected by nematode species. Significantly higher numbers of IJ were...
Jiji Li*, Tianyang Zu, Xiangli Dong, Xiao Yang, Wei Liu and Changwen Wu
...r fish, and revealed the infectivity of the large yellow croaker. Thus, these findings could help to develop methods of disease resistance in the aquaculture industry.
...
Ali Murad Rahoo1, Tariq Mukhtar2,*, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
...epends on their level of infectivity and reproductive capacity. Nematodes with higher levels of infectivity and reproduction within a specific target host may be more effective in controlling a particular insect under field conditions. The production of infective juveniles (IJ) from Galleria mellonella cadavers may differ among different species of EPN and hosts of various sizes. Therefore, the objective of conducting...
Ejaz Mahmood Ahmad Qureshi1,*, Amtul Bari Tabinda2, Seemal Vehra3 and Atif Yaqub4
...st (ELISA). To determine infectivity in a mosquito population, detection of the viruses in adult mosquitoes was used to calculate Minimum Infection Rate (MIR). Average MIR was highest being 3.52 and 2.94 in late rainy season in 2011 and 2012, respectively, while it was highest (3.06) in early post rainy season in 2013. Results also revealed significant correlation coefficient ‘r’ and coefficient ofdetermination r2 of MIR with air tempera...
Ali Murad Rahoo1,*, Tariq Mukhtar2, Barkat Ali Bughio3 and Rehana Kanwal Rahoo4
Shaikh Abdul Lateef, Zhou Lin, Wu Jian, Junjie Qian, Jonathan Hartanto Tan and Zheng Shusen*
...rchitecture, life cycle, infectivity mechanisms, immune escape strategies, disease epidemiology and preventive measures associated with HBV. The current antiviral therapeutics are not providing adequate protection against HBV. It is mandatory to characterize and devise novel antiviral therapies to control the spread of hepatitis ...
Abeer A. Feisall; B.A. Othman2; Kh.A. El-Dougdoug2 and H.S. Shalaby1
...and 9.The virus lost its infectivity after
exposure to UV for 50 min. at distance of 53 and 70 cm from the UV source. The
bacteriophage (St-1) was inactivated in presence of NaCl at concentration higher
than 15%; sodium benzoate at concentration of 0.5%; potassium sorbate at
concentration of 1.0 % and citric acid at concentration of 1.0% as well as in
presence of 5% of either Sodium hypochlorite or SD...
D. N. Abd-elshafy1, 2 and M. M. Bahgat2, 3
...ate of the virus. Plaque infectivity count assay was carried out using safe concentrations of the cocktail protease inhibitor MixG and its five components: ABESF, aprotinin, E-64, leupeptin and EDTA. Also quantification of the activities of both intracellular and secreted HepG2 proteases was carried out using specific chromogenic substrate at different ranges of pH values. Results showed that EDTA has intracellular metalloprotease inhibitory effect that enhanc...
Abd El-Hamid, M.I1, Seham, A.El-Zeedy1, El-Sanousi, A.A2, Reda, I.M2, Nehal, S. Saleh1, Abbas, A.M1
... essential role in virus infectivity and its function in entry of virus into cells and is considered as one of the most potent inducers of virus-neutralizing antibody among the spectrum of EHV-1 proteins .A wave of Abortions has been recorded in El Zahraa stud for Arabian Horses ,collected samples were either aborted fetal tissues either Lung and livers as well as Aborted placenta and nasal swaps from mares suffering from repeated Abortions, The current study ...
Abd El-Hamid, M.I1; Seham, A. ElZeedy1; El-Sanousi A.A2, Reda, I.M2, Nehal, S. Saleh1., Abbas, A.M1
...due to its role in virus infectivity and its function in entry of virus into cells and is considered as one of the most potent inducers of virus-neutralizing antibody among the spectrum of EHV-1 proteins based on sequence analysis and multiple alignment revealed single nucleotide substitution at the base pair number 121 from the start codon which give lead to single Amino Acid Substitution from CAG (Glutamine(Gln/Q)) which is considered as a polar Amino Acid t...
Ayman S. El-Habbaa1 and Mervat E. Radwan2
...d for cattle
infectivity and responsible for its persistence in the endemic areas. Such epidemiological data could
guide the application of efficient control strategies of BEF virus in Egypt.
... 
Reem M. Ramadan1, Fady Sayed Youssef2, Gehad Genidy Mohamed3, Sameh Hamed Ismail4, M.M. El-Bahy1, Shimaa Abdel-Radi1*
...destructive effects. The infectivity of sporulated oocysts exposed to LC50 & LC90 were evaluated by In vivo inoculation of broiler chicks. The results demonstrated that the increase in the rate of sporulation inhibition is directly related to the increase in C-Oo.Nc concentrations and exposure time. Analyzing the DNA damage in the oocysts exposed to different concentrations using comet assay revealed a significant direct variation (p ≤ .05) between the ...
S. Feroza1, A. G. Arijo1† and I. R. Zahid2
...seeds on Ascaridia galli infectivity in broiler chicken. A total of eighteen broiler birds were randomly selected that were divided into three groups (A, B and C) with 6 birds in each group. The birds were then artificially infected with Ascaridia galli @ 2000 eggs/bird. Ethanolic extracts of papaya and neem were applied to Group B and C, respectively while Group A was left untreated that served as control. The fecal egg count (FEC) was conducted on ...
A. M. Rahoo1,*, †, T. Mukhtar2, S. I. Abro3, S. R. Gowen1 and B. A. Bughio4
...nt in
soils, infectivity, persistence and pathogenicity for longer periods. In the present study, emergence of Steinernema
feltiae infective juveniles (IJs) from infected Galleria mellonella cadavers was monitored under moist and dry
conditions at 5 and 10oC. Greater numbers of IJs of S. feltiae recovered from G. mellonella cadavers kept at 10oC
than from those kept at 5oC. Likewise, significantly greater number ...
A.E. Ismail† and A.M. Kheir*
...valuate its influence on infectivity, development and
virulence of Heterodera zeae. The results revealed that the nematode final population and rate of build-up
correlated negatively with the host age. The nematode multiplied highly on the youngest seedlings; and was more
virulent on the youngest than on the oldest plants.
...
F. Shahina†, K.A. Tabassum and M.A. Habib*
... were assessed for their infectivity against the
cotton bollworm complex in field. EPNs cultured on Galleria mellonella L. and stored in distilled water at 5 °C,
were kept at room temperature for 24 hrs before use. The number of bollworms on plants before and 24 hrs after
EPN spray @ 1000 and 2000 juveniles/ml water were assessed for mortality percentage. All four species of insects,
viz., Helicoverpa armiger...
A. N. Mahar, N. D. Jan†, A. Q. Mahar and S. R. Gowen
...eniles (IJs) and maximum infectivity of black cricket nymphs as compared to other nematodes at 25 °C. On the other hand, both Heterorhabditids caused maximum mortality, production and infectivity as compared to two Steinernematids at 30 ° C. When different dose concentration levels were tested in the sand arena, all dose concentrations resulted satisfactory mortality but a high level of dose concentration (400 IJs / ...
S.A. El-Kewey1; R.A. Omar1; S.A. Sidaros2 and Samaa Abd El-Khalik3
... determined as 65oC, and infectivity was retained for one day only. The virus transmitted mechanically, and by means of Myzus persicue and Aphis fabue. Green peach aphid was found to be more effective in virus transmission than broad bean aphid. Virus transmission through vegetative propagation reached 100% in both cultivars (Chinese and Balady) with different degrees of symptoms severity. Plant height, leaf area. fresh and dry weight of bulb decreased signifi...
Ahmed, AmalA.; Zein, Salwa N. and Khatab, Eman A. H.
* Amal Abou El-Ela, A.
...he dilution end-point of infectivity was 10-3, the thermal inactivation point was 540C, and longevity in vitro was of 10-14 h at 250C. PNRSV was detected by double antibody sandwich-enzyme linked immunosorbent assay (DAS-ELISA). Immunocapture-reverse transcription-polymerase chain reaction (IC-RT-PCR) was used to amplify a 450 bp cDNA fragment from infected rose leaves (Rosa indica L.) using the primers Macl and Mac2 specific to Prunus necrotic ring spot virus...
Amal Abo El-Ela Ahmed; Eman A.H. Khatab; and M.S. Shafie
E.F. Mohamed1 and I.M. Sabra2
...in vitro and in vivo the infectivity of ToMV to a certain extent expressed as the number or local lesions induced by ToMV on Nicotiana sylvestris. This reduction increased gradually by increasing GA and IAA concentrations. ToMV inhibition percentages induced by GA and IAA varied according to the time of treatment (1, 2, 3, and 4 hours). High percentages of inhibition were recorded for in Vitro treatment. ToMV inhibition of pre-inoculation treatment was higher ...
E.F. Mohamedl and A.A. Owayss2
...d to Fabaceae. BBMV lost infectivity after 10 min at 95 on dilution of 10-3 and after 21 days storage at room temperature. BBMV was easily transmitted by sap. ELISA was used efficiently to detect BBMV in leaves of infected Faba bean plants. The present study was carried out to determine the effect or propolis on BBMV. In vitro experiment, propolis reduced the occurrence of mosaic symptoms on Faba bean plants to 80, 70, 60, 75 and 90% of infected plants immedia...
