Antibacterial Efficacy of Manuka Honey against New Delhi Metallo-β-Lactamase Producing Gram Negative Bacteria Isolated from Blood Cultures
Antibacterial Efficacy of Manuka Honey against New Delhi Metallo-β-Lactamase Producing Gram Negative Bacteria Isolated from Blood Cultures
Muhammad Usman Qamar1, 2, Sidrah Saleem1, Usman Arshad1, Muhammad Farhan Rasheed3, Hasan Ejaz4, Naveed Shahzad5 and Shah Jahan6,*
Fig 1
Modified Hodge’s test for phenotypic carabpenemase detection. T, test organism; P, positive control (K. pneumoniae); N, negative control (E. coli).
Fig 2
Double disc potentiation method for the phenotypic detection of MBL producing bacteria. Positive results are shown by enlarged zone of inhibtion. MEM, meropenem disc; ETA, ertapenem disc; EDTA, ethylene-diamine tetra acetic acid.
Fig 3
PCR product of blaNDM-1gene on 1.5% agarose gel. N, negative control; P, positive control; T1-T5, test sample; L, DNA ladder.
Fig 4
Agar well diffusion assay for the determination of antibacterial activity of manuka honey. White arrows indicate the zone of inhibition (mm) produced by undiluted manuka honey against NDM-1 producing bacteria.
December 2017
Vol. 49, Iss. 6, Pages 1937-2341
