Avian Coronaviruses in Wild Birds along Brazilian Segment of Atlantic Flyway
Avian Coronaviruses in Wild Birds along Brazilian Segment of Atlantic Flyway
Barbosa Carla1*, Gregori Fabio2, Thomazelli Luciano1, Oliveira Amanda1, Araújo Jansen1, Ometto Tatiana1, Marcatti Roberta3, Nardi Marcelo3, Paludo Danielle4, Utecht Nathalia1 and Edison Durigon1
ABSTRACT
Degradation of natural habitats has been described by many previous studies as a major factor in the transmission of infectious diseases, once it leads to changes on migration patterns and dispersal of birds, as resting and feeding points become scarcer resulting in agglomeration of different species. One pathogen of greatest importance that may be dispersed by birds are the coronaviruses (CoVs). Despite its relevance and complexity, studies on the detection and genetic characterization of these viruses are scarce, especially in South American wild birds. Thus, this paper aims to detect and discuss the diversity of these agents in birds at migratory stopping and wintering sites, along Brazilian Segment of the Atlantic Flyway. Therefore, 738 avian samples from different regions of Brazil were subjected to a RT-Nested-PCR protocol, with primers targeting RdRp region. Positive samples were confirmed using Sanger followed by Ion Torrent S5 sequencing platforms and results were submitted to phylogenetic analysis. From total of 8 confirmed samples, 3 clustered to Deltacoronavirus genus and 5 to Gammacoronavirus. Two of those samples showed partial sequences of other regions of CoVs confirming their genotyping to Gammacoronavirus genus and proximity to important poultry diseases causative agents.
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