Cardioprotective Effect of Naringenin against Myocardial Ischemia-Reperfusion Injury via Alteration of Apoptotic Signaling Pathway
Cardioprotective Effect of Naringenin against Myocardial Ischemia-Reperfusion Injury via Alteration of Apoptotic Signaling Pathway
Tao Ren1, Guiqiu Cao2, Xiao Han3, Feng Tan1, Qiaoli Chen4, Shicheng Yang5 and Haiyan Zhang6*
ABSTRACT
Ischemic heart disease (IHD) is a common multiple cardiovascular disease (CVD) in clinical settings. It is a major contributor to mortality and morbidity worldwide and causes a serious threat to human life and health. Naringenin, a flavonoid possesses the potent antioxidant potential and it is proposed to be useful in the treatment of CVD. In this experimental study, we aimed to scrutinize the cardio-protective effect of naringenin against the I/R induced myocardial injury and elucidate the possible mechanism of action. In vitro studies, the H9c2 cardiomyocytes cells were treated with naringenin or without naringenin and then subjected to I/R, respectively. At end of the experimental study, the rats were anesthetized and blood samples were collected to scrutinize the various parameters such as creatine kinase myocardial band (CK-MB), high-density lipoprotein (HDL), lactate dehydrogenase (LDH), creatine, troponin-T (TRT), cholesterol, C - reactive protein (CRP), and concentration of mitochondrial enzymes viz., Ca2+, Na+ and K+ ions were estimated in blood. Heart tissue was also isolated for caspase-3 activity. Our result showed that naringenin pretreatment significantly increased cardiac dysfunction via scavenged free radicals and a reduction of inflammatory reaction. Dose-dependent treatment of naringenin significantly altered the CK-MB, HDL, LDL, creatinine, cholesterol, CRP, Ca2+, Na+ and K+, respectively. A significant alleviating change in these biochemical parameters along with caspase-3 activity was noticed. Thus, in our study, we determined that I/R induced cardiac remodeling can be successfully mitigated by naringenin.
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