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Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Comparative Evaluation of Elisa Using A Semi-Nested 16SrRNA Pcr as a Master Test for Detection of Helicobacter Pylori Antigen in Human Stool and Feces of Dogs and Cats

Neven Waheeb1*, Sherif Marouf2, Essam Nasr3, Shaymaa Abdelmalek2 

1Veterinary Serum & Vaccine Research Institute - VSVRI, Egypt; 2Microbiology and immunology Faculty of Veterinary Medicine, Cairo University, Egypt; 3Head of Bacteriology Diagnostic Products Department Tuberculosis)Veterinary Serum and Vaccine Research Institute Abbasia, Cairo **Vice Chairman of the Vaccine Research Institute Abbasia, Cairo, Egypt.

*Correspondence | Neven Waheeb, Veterinary Serum & Vaccine Research Institute - VSVRI, Egypt; Email: [email protected] 

Figure 1

Agarose gel electrophoresis (1%) of 16sRNA using nested PCR showing; first run of heminested PCR using outer primers showing 1024 bp product and second run PCR using heminested primers. 250 bp product. 

Advances in Animal and Veterinary Sciences

1

Adv. Anim. Vet. Sci., Vol. 13, Iss. 1, pp. 1-216

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