Research Article

Determination of Chemical Composition of Essential Oils Extracted from Conventional and Organically grown Basil (Ocimum Basilicum) from Different Geographical Regions

Riaz Shah1*, Shaikha Hilal Al Ismaili2, Sheikha S. Al-Siaby1, Amal Mohammed Al Nasiri2, Thuraiya Hafidh Al Maskari2, Jamal AlSabahi3 and Huda Al-Ruqaishi3

1College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud-123, Oman, Muscat, Sultanate of Oman; 2College of Science, Sultan Qaboos University, Sultanate of Oman; 3Central analytical laboratory, College of Agricultural and Marine Sciences, Sultan Qaboos University, Sultanate of Oman.

Received | October 01, 2020; Accepted | December 12, 2021; Published | February 24, 2022

*Correspondence | Riaz Shah, Department of Plant Sciences, College of Agricultural and Marine Sciences, Sultan Qaboos University, Al-Khoud-123, Oman, Muscat, Sultanate of Oman. Email: [email protected]

Citation | Shah, R., S.H. Al-Ismaili, S.S. Al-Siaby, A.M. Al-Nasiri, T.H. Al-Maskari, J. Al-Sabahi and H. Al-Ruqaishi. 2022. Determination of chemical composition of essential oils extracted from conventional and organically grown Basil (Ocimum Basilicum) from different Geographical Regions. Sarhad Journal of Agriculture, 38(2): 532-539.

DOI | https://dx.doi.org/10.17582/journal.sja/2022/38.2.532.539

Keywords | Dried basil, Essential oils, GCMS, Microwave extraction, Ocimum basilicum, Volatile compounds

Introduction

Plants are used as food additives, pharmaceuticals, fragrances and for herbal medicine purposes (Lucchesi et al., 2004; Politeo et al., 2007). Basil Ocimum basilicum (Lamiaceae) called Rehan in Arabic, is one of the aromatic herbs that are grown and used in most of the countries around the world (Politeo et al., 2007; Hanif et al., 2011). Basil leaves have distinctive aroma and both fresh and dried forms are used as a spice in meals. Fresh or dry basil are used as medicinal herbs to treat some diseases such as headaches, constipation, inflammation and coughs (Simon et al., 1999; Opalchenova and Obreshkova, 2003). Moreover, basil is believed to have antimicrobial, antioxidant and anticarcinogenic activity (Nurzynska-Wierdak, 2013).

The presence of essential oils and their composition determines the specific aroma of a plant. The presence of essential oils present in basil aerial parts contain 1,8-cineole, linalool, methyl cinnamate and methyl chavicol which are attributed to its aroma (Klimánková et al., 2008; Nurzynska-Wierdak, 2013; Bufalo et al., 2015).

Several techniques have been developed to extract the EO and volatile compounds from plants (Lucchesi et al., 2004). Traditional extraction methods have some difficulties and compounds like monoterpenes can undergo chemical changes or most volatile compounds can be lost during the solvent removal step (Lucchesi et al., 2004; Klimánková et al., 2008). A microwave extraction system was used in this study which heats the solvents and partition analytes from the sample matrix. This process uses least amount of solvent and there is no solvent removal step. Microwave-assisted extraction technology has been used to extract compounds from plants which facilitates the diffusion of the compounds into the extracting solvent (Flórez et al., 2015; Adetunji et al., 2017).

Composition of essential oil is influenced by many factors including the cultivation practices, cultivars, origin of the plant, colour of leaves, and the drying and extraction methods (Hussain et al., 2008; Bufalo et al., 2015). Geographical origins in particular affect compositions of essential oil. There are few studies that compare composition of essential oils extracted from commercially available fresh and dried, and organic and conventional basil. This study determines the essential oil volatile chemicals composition extracted from basil of different origin, state (fresh or dried) and growing conditions (organic or conventional) available in Oman using Gas Chromatograph Mass Spectrometer (GCMS).

Materials and Methods

Plant material

Intact plant in pots of conventional Omani and Indian basil and organic basil imported from Belgium (Vegobel) were purchased from a local market in AlKhoud, Oman. Pre-packaged (dried) conventional basil imported from United Arab Emirates (UAE) (Bayara) and organic basil originating from Egypt were also purchased from local market.

Essential oils extraction

Fresh Omani, Indian and Organic basil leaves (100 g) were placed in the microwave container to which distilled water (2.5 mL) was added before putting in the Milestone’s Ethos X microwave extraction system (Milestone, Italy) for 30 min at 280W. Dried basil (100 g), both conventional and organic, with distilled water (2.5 L) was kept in the microwave extraction system for 30 min at 280W. Water is a preferred solvent because it is safe and inexpensive. The extracted essential oil was collected, measured and stored at 4 °C until injection into GCMS. The volume of collected oil was then converted to mass using the known density of basil (0.957 g/ml at 25 °C). The yield of essential oil in percentage was calculated as follows:

Essential oils composition by GCMS

A drop of each of basil essential oil was added to 1 mL of hexane in separate vials and 1 μL with split ratio of 100:1 was injected into GCMS. The chemical composition analysis was achieved using Perkin Elmer Clarus 600 GC System, Perkin Elmer Clarus 600C MS with quadrupole mass analyzer and Rtx-5MS capillary column (30 m x 0.25 mm i.d.; film thickness 0.25 μm; 350 oC maximum temperature). Injection temperature was set as 280 oC and that of transfer line and ion source was 270 oC. Helium (99.9999% purity) was used as a carrier gas with constant flow rate of 1 mL/min. Ionization energy was 70 eV, mass spectra scan range was 40-550 amu and 60 oC oven temperature programmed rate of 8 oC per min to 280 oC and 25 min hold. The run time was 53.5 min. Components were identified using NIST 2011 v.2.3 and Wiley (9th edition) mass spectrum libraries.

 

Table 1: Yield of essential oil from different types of basil using advanced microwave extraction method.

Type of basil	Volume (µL) per 100 g	Yield (% v/w)
Fresh conventional Indian basil	475c	0.47c
Fresh conventional Omani basil	675d	0.67d
Fresh organic Belgian basil	325a	0.32a
Dried conventional basil (UAE)	375b	0.37b
Dried organic basil (Egypt)	388b	0.38b

 

Results and Discussion

Yield of essential oil

There was significant difference (F=35.9; df=4,9; p<0.001) in the amount of essential oil extracted from different samples (Table 1). The highest yield of essential oils was obtained from the conventional fresh Omani basil (675±25 µL) and the lowest from the organic fresh basil (325±12 µL). Dried conventional (UAE) and organic (Egypt) basil had similar oil yields.

Essential oil composition

Essential oil composition of organic and conventional fresh and dried basil (O. basilicum L.) from different geographical sources was identified using GCMS. The relative content of each volatile component is expressed as a relative percentage (100% is equivalent to the sum of all 42 detected compounds. A total of 42 compounds were detected in the essential oils from all the basil samples of which 38 were identified; 35 from fresh organic Belgian basil, 28 from dried organic basil (Egypt), 28 from dried conventional basil (UAE), 24 from fresh conventional Indian basil and 21 from fresh conventional Omani basil (Table 2 and Figure 2). The detected compounds included sesquiterpene hydrocarbons (α-bergamotene, β -caryofyllen, α-humulene), benzenoid compounds (methyl chavicol), monoterpene hydrocarbons (α-pinene, etc.) and oxygenated products (1,8-cineole, linalool, and camphor). Among the total volatile compounds 14 were common in all the basil types, which were α-pinene, camphene, β-pinene, cineole, β-linalool, camphor, α-terpineol, β-elemen, α-bergamotene, β-eudesmene, δ-guaiene, γ-muurolene, δ-cadinene and δ-cadinol. β-linalool was the main compound found in the essential oils of all basil types used (31.74-80.47%) except for dried conventional basil imported from UAE where it was the second most abundant after α-terpineol.

The specific aroma and flavour of plants depends on the presence and composition of essential oils. Different factors affect the essential oil yield and its chemical composition. These factors include different cultivars and their growing conditions (conventional vs organic) and, harvesting, drying and extraction techniques (Merk et al., 1988; Muzika et al., 1989; Klimánková et al., 2008; Lee et al., 2005).

The essential oil yield of 475-675 µL per 100 g in our study is comparably to 450-1110 µL per 100 g extracted from different cultivars of fresh conventionally grown basil. However, a volume of 325 µL per 100 g reported from fresh organic Belgian basil is lower than the 400-1100 µL per 100 g reported for the same cultivars of fresh organic basil (Klimánková et al., 2008; Bufalo et al., 2015). The reason for the lower quantity of EO from fresh organic basil in study may not be because of the growing conditions (conventional vs organic) but because of difference in cultivar and the long time spent between transit and sample analysis. The fresh Indian basil also had lower yield of EO because of the same reason. The essential oil components are volatile and increase in the time between harvesting/dispatch and analysis had decreased the yield of EO (Klimánková et al., 2008). Moreover, the growth conditions during transit are also not optimal for the plant (when whole plants are shipped).

On other hand the drying process will also reduce the quantity and composition of EO. Various physicochemical processes, e.g. oxidation, evaporation, etc during the drying change the aromatic volatile compounds influencing the aroma and the quality of the dried products (Jerković et al., 2001). In dried basil a decrease in sesquiterpene hydrocarbons, benzenoid compounds, and monoterpene hydrocarbons and increase in oxygenated products has been reported (Klimánková et al., 2008). The exact composition and drying techniques of the commercially-available dried basil used in our study were unknown and this might be the reason of varied composition (Chalchat and Özcan, 2008).

Direct comparison of fresh vs dry samples from different region, keeping in mind the available data, is not reliable because there are too many parameters to compare. We, therefore avoided to focus too much on this comparison, but focused more on the yield and composition.

Several studies were conducted to identify the chemical composition of basil from many regions of the world. The profile of O. basilicum oil determined in this study is almost similar to many studies (Klimánková et al., 2008; Zheljazkov et al., 2008; Chenni et al., 2016). One study on chemical composition of Omani basil indicated linalool (69.9%) as the main compound while geraniol, 1,8-cineole, bergamotene and geranyl acetate were also found (Hanif et al., 2011). These values are similar to our findings and the variations could be contributed to the cultivar, extraction process (Clevenger type distillation apparatus) and the part of the plant used (whole plant with flowers). Linalool, 1,8-cineole and eugenol were also identified as dominant components in Egyptian basil oil (Ismail, 2006). The essential oil of a native northeast Indian O. basilicum had very high camphor (42.1%)

 

Table 2: Volatile compounds identified in different basil samples determined by GC-MS and expressed as a relative percentage. 100% is equivalent to the sum of all identified compounds for that sample.

S. No.			Basil type
			Conventional			Organic
	Name	tR (minutes)	Fresh (Omani)	Fresh (Indian)	Dried (UAE)	Fresh (Belgian)	Dried (Egypt)
1	α-Pinene	4.19	1.36	0.76	1.66	9.40	6.78
2	Camphene	4.43	0.08	0.06	0.08	0.31	0.30
3	2,4- Thujadiene	4.52	--	--	--	0.15	0.13
4	β-Pinene	4.86	0.83	0.83	0.49	0.83	0.95
5	Octamethyl cyclotetrasiloxane	5.02	--	--	0.13	0.19	0.12
6	4-Carene	5.43	--	0.28	0.06	0.20	0.30
7	1,8-Cineole	5.72	6.46	5.49	5.93	5.60	8.55
8	γ-Terpinene	6.40	--	0.20	0.11	--	--
9	β-Ocimene	6.52	--	--	--	--	0.30
10	Fenchone	6.83	--	0.06	0.09	--	--
11	β-Linalool	6.88	80.47	75.14	13.12	31.75	46.43
12	Camphor	7.69	0.48	0.50	0.47	1.36	0.89
13	Isoborneol	8.10	--	--	0.08	0.27	0.28
14	Terpinen-4-ol	8.43	--	0.15	0.26	0.52	0.57
15	α-Terpineol	8.72	0.45	0.56	71.58	0.62	0.79
16	Tarragon	8.96	--	--	--	5.77	--
17	Estragole (methyl chavicol)	9.26	0.74	0.16	--	--	7.26
18	Geraniol	10.06	4.81	7.11	--	--	--
19	Bornyl acetate	11.17	--	--	0.37	2.88	1.40
20	Unidentified	11.57	--	--	0.06	0.32	1.31
21	Copaene	11.81	--	--	0.04	0.16	0.13
22	β-Elemen	12.10	0.19	3.98	0.40	3.90	1.06
23	Caryophyllene	12.34	0.14	--	0.72	--	1.13
24	Methyl eugenol	12.44	--	--	--	10.08	--
25	α-Bergamotene	12.52	2.01	2.49	1.19	5.14	3.97
26	(E)-β-Farnesene	12.79	--	0.05	--	1.39	--
27	Humulene	12.87	0.05	0.07	0.35	--	--
28	α-Caryophyllene	12.93	0.08	0.04	--	1.75	--
29	β-Gurjunene	13.12	--	--	--	0.34	--
30	Unidentified	13.21	--	--	--	0.15	--
31	Germacrene D	13.30	--	--	--	2.19	--
32	β-Eudesmene	13.44	0.33	0.26	0.56	0.75	1.82
33	Eremophilene	13.57	--	--	--	1.17	--
34	δ-Guaiene	13.70	0.23	0.29	0.31	0.72	0.84
35	γ-Muurolene	13.82	0.14	0.12	0.10	1.97	0.44
36	δ-Cadinene	13.95	0.27	0.27	0.37	0.40	1.63
37	Unidentified	14.55	0.03	0.09	0.15	0.18	--
38	Unidentified	14.71	0.07	--	0.03	0.12	--
39	Spathulenol	14.77	--	--	--	0.40	0.16
40	Caryophyllene oxide	15.21	--	--	0.31	0.80	1.40
41	Unidentified	15.55	--	--	--	1.28	1.38
42	δ-Cadinol	15.61	0.77	1.05	0.98	6.97	7.62

 

(Purkayastha and Nath, 2006). The main constituents of the basil oil from French Polynesia were (E)-methyl cinnamate (62.3%) and (Z)-methyl cinnamate (8.6%) (Adam et al., 2009). Methyl chavicol (37.6-43.0%) and linalool (28.9-33.4%) were found as the major compounds in the EO in two varieties of O. basilicum from Iran while another variety was rich in geranyl acetate (45.6%) but low in linalool (25.6%) (Safari et al., 2014). Different cultivars may show a similar pattern of volatile compounds but the relative amount of major components, for example, linalool, eugenol, 1,8-cineole, and bergamotene, etc usually differ (Klimánková et al., 2008).

 

The different volatile compounds found in essential oil of basil have different properties. Many authors reported β-linalool as the main component of basil essential oil in previous studies (Mondello et al., 2002; Telci et al., 2006; Hussain et al., 2008; Bufalo et al., 2015). European basil oil especially Bulgarian basil oil is considered as a high quality essential oil due to higher linalool contents (71.4%) (Jirovetz et al., 2001; Sifola and Barbieri, 2006). Linalool is a terpene alcohol found in many spices and flowers and has anti-inflammatory, antibacterial, antifungal and antiviral properties (Politeo et al., 2007). Linalool due to its pleasant scent has been used in perfumed hygiene products and cleaning agents including soaps, detergents, shampoos, and lotions.

Essential oils extracted from aromatic plants has a monoterpene called geraniol. Geraniol has been found effective against breast, liver, colon, prostate, pancreatic, skin, lung and kidney cancers (Koziol et al., 2015; Cho et al., 2016). Geraniol is classified as safe food additive by the Food and Drug Administration (FDA) of the United States. Geraniol is also approved for use within allergenic epicutaneous patch tests used as an aid in the diagnosis of allergic contact dermatitis (ACD).

β-elemene belongs to the class of organic compounds known as elemane sesquiterpenoids which has antibacterial properties (Noriega et al., 2019). Methyl chavicol (estragole) have shown antioxidant and antilipase activities which may make it useful in the treatment of diseases (Panten and Surburg, 2018). Isobornyl acetate is used in perfuming soap, bath products, and air fresheners, and production of camphor (Purkayastha and Nath, 2006). 

A strong negative correlation (r = -0.88; r2 = 0.77; p = 0.04) was observed between the amount of oils extracted and the number of compounds detected. The fresh organic basil had yielded the least oil but had the highest number of compounds. Although the EO yield of the fresh organic basil was very low due to long transit and poor growth conditions the compounds present in the EO did not deteriorate and we were able to extract them. The essential oil extracted from conventional and organic basil did not differ much in terms of profile but composition did differ. Organic basil had much higher amount of α-pinene and δ-cadinol compared to conventional basil while β-linalool was high in conventional basil (Figure 1). Some additional compounds were detected only in organic basil (Egyptian and Belgian) although in very low percentages (0.12-2.87%). Examples of these compounds were spathulenol, 2,4-Thujadiene, caryophyllene oxide, octamethyl cyclotetrasiloxane, bornyl acetate, isoborneol and caryophyllene. Methyl eugenol was found only in organic fresh Belgian basil samples. We did not detect methyl chavicol in fresh organic basil and a significantly lower level of methyl chavicol has been reported in one organically grown cultivar compared to the same cultivar grown conventionally (Klimánková et al., 2008). Some reports demonstrated that organic and conventional fertilizers or growing conditions did not modify the profile composition of sweet basil essential oils but the relative percentages of volatile compounds were found different among cultivars (Klimánková et al., 2008; Bufalo et al., 2015).

The difference in the amounts and the types of volatile compounds from basil from different countries in current study may be due to genetic factors, local environmental conditions, plant nutrition and different genotype (cultivars) (Klimánková et al., 2008). Apart from

 

many factors, linalool, 1,8-cineole, methyl cinnamate, and methyl chavicol are the main volatile compounds responsible for the typical basil aroma (Lee et al., 2005). Based on essential oil chemotypes classification by Lawrence (1988), Omani and Indian fresh basil was linalool-rich, Belgian organic basil was methyl eugenol-rich, Egyptian dried basil was methyl chavicol-rich (Lawrence, 1988). UAE dried basil was terpineol-rich.

Conclusions and Recommendations

The essential oil yield and component profiles were seen to be responsive to the plant origin and/or growing environment. A total of 14 volatile compounds were common in all the basil types; α-pinene, β-pinene, camphene, cineole, β-linalool, camphor, α-terpineol, β-elemen, α-bergamotene, β-eudesmene, δ-guaiene, γ-muurolene, δ-cadinene and δ-cadinol. β-linalool was the main compound identified in the essential oils of analyzed basil types used (31.74-80.47%). Higher number of volatile compounds was detected in organic compared to commercially grown basil.

Novelty Statement

The fourteen stable compounds identified from basil could be considered as chemical markers for basil. Higher number of volatile compounds were detected in organic compared to commercially grown basil.

Authors’ Contributions

Riaz Shah: Prepared and edited the manuscript.

Shaikha Hilal Al-Ismaili, Sheikha S. Al-Siaby, Amal Mohammed Al-Nasiri and Thuraiya Hafidh Al-Maskari: Arranged and prepared samples, extracted essential oils and conducted GCMS analysis.

Jamal Al-Sabahi and Huda Al-Ruqaishi: Helped in GCMS analysis and requisition relevant data.

Conflict of interest

The authors have declared no conflict of interest.

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