Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Diagnosis of Staphylococcus aureus Infection in Bovine Mastitis using its Affinity Purified Fraction
Ehab A. Fouad1*, Khaled A. Abd El-Razik2 , Eman H. Abdel-Rahman3
SDS PAGE profile of S. aureus purified fraction showed 2 bands (2 peaks).
SDS PAGE profile of S. aureus Crude Antigen show 10 bands (10 peaks).
Mean OD values of cows’ positive sera against the crude antigen of S. aureus.
Mean OD values of cows’ positive sera against the purified fraction of S. aureus.
Potency of the purified fraction of S. aureus to antibodies of positive cows’ milk samples.
Significant validity of the stored purified fraction of S. aureus for one year at -20oC.
Validity evaluation of the stored purified (Series 2) S. aureus fraction for one year at -20oC compared with freshly purified fraction (Series 1).
Specificity evaluation of S. aureus purified fraction in comparison with different antigens in diagnosis of mastitis.
