Effect of Lab Prepared and Commercially Available Activation Media on the Milt Quality and Fertilization Rate of Clarias gariepinus (Bruchell, 1822) African Catfish
Effect of Lab Prepared and Commercially Available Activation Media on the Milt Quality and Fertilization Rate of Clarias gariepinus (Bruchell, 1822) African Catfish
Zarifshan Malik1, Muhammad Ramzan Ali2*, Hasina Basharat2, Aziz Ahmed2 and Sabina Noor1
ABSTRACT
African Catfish (Clarias gariepinus) is an improved source of nutrition because of its high biological value, high protein deposition compared to the fat content and low cholesterol level. African catfish is cultured widely all over the world through artificial propagation, but male gametes cannot be collected through simple hand stripping method. Therefore, the males require to be sacrificed in order to get the milt from the testes. As millions of sperms are present in per ml of milt so, in order to utilize milt more efficiently, various researches are based on use of different types of activation or dilution media. Therefore, the lab prepared activation media A and B, commercial activation media C and fresh water were used in this experiment to compare their effects. Experimental layout was CRD (Complete Randomized Design) with four treatments: control, media A, media B and media C (Actifish) with three replications for each. The study was conducted at National Agriculture Research Center (NARC), Islamabad, Pakistan. Seeds of African catfish were brought from Thailand which underwent acclimatization under the local culture condition. After attaining sexual maturity, six males and five females were selected for the study. Milt was collected and diluted using the above-mentioned activation media by using dilution ratio of 1:29. Sperm quality assessment of diluted milt was carried out for different traits such as sperm motility, sperm motility duration and sperm viability. Fertilization rate was determined by using same diluted milt aliquots. The results indicated that sperm quality indices and fertilization rate of milt treated with activation media show better results in comparison to control. Best results with respect to all parameters were obtained from experimental group treated with Commercial activation media (C) while lowest values were obtained for control group. The commercially available, Activation media could be useful for increasing the seed production in commercial level.
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