Epidemiological Surveillance for the Newly Classified Newcastle Disease Virus Genotype VII.1.1 in Chicken Flocks in Egypt
Epidemiological Surveillance for the Newly Classified Newcastle Disease Virus Genotype VII.1.1 in Chicken Flocks in Egypt
Sameh Abdel-Moez Ahmed Amer1*, Mohamed Abdel-Aziz Kutkat1, Mohamed Mahmoud Abdel-Baki1, Asmaa Mahmoud Maatouq1, Omnia Mohamed Kutkat2, Hagar Magdy Ahmed1, Khaled Mohamed El-Bayoumi1
Visualization of 1.5 % agarose gel electrophoresis NDV full F-gene amplification product.
M: 1 kb DNA ladder; Lane 1: Control positive for NDV; Lane 2and3: Negative NDV samples; Lane 4 and 5 and 6 and 7 and 8 and 9: Positive NDV specific bands of 1681 bp amplicon.
Partial amino acid alignment of the of F- gene cleavage site (112 to 117) of the identified NDV isolates of our study and other NDV strains circulating in Egypt related to velogenic NDV genotype VII 1.1, as well as other vaccinal strains related to NDV genotype II using Bio-edit program. The dot (.) represents identity while single alphabet represents the difference in the amino acid sequence. Underlined isolates are isolates of our study.
Phylogenetic relationship between the NDV isolates obtained in this study and other previously isolated in Egypt with some reference and vaccinal strains retrieved from the Genbank by Maximum likelihood method using Lasergene® software. Isolates of the study.
Amino acid (A.A) sequence identity of obtained NDV isolates with NDV strains circulating in Egypt and NDV vaccines showing identity and divergence percent based on A.A sequence comparison using Lasergene® software, black squares indicate identical sequence.
