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Exploration of Polymorphism Analysis of CD18 Gene with PCR-RFLP Technique in Friesian Holstein Crossbreed Dairy Cows in Batu, East Java, Indonesia

Exploration of Polymorphism Analysis of CD18 Gene with PCR-RFLP Technique in Friesian Holstein Crossbreed Dairy Cows in Batu, East Java, Indonesia

Tiara Widyaputri*, Aldryan Cristianto Pratama, Dian Vidiastuti, Yudit Oktanella

Faculty of Veterinary Medicine, Brawijaya University, Jl. Puncak Dieng, Kunci, Kalisongo, Dau, East Java, Indonesia, 65151

 
*Correspondence | Tiara Widyaputri, Faculty of Veterinary Medicine, Brawijaya University, Jl. Puncak Dieng, Kunci, Kalisongo, Dau, East Java, Indonesia, 65151; Email: [email protected]

Figure 1:

Electrophoresis results for DNA isolate 1% agarose concentration. Note: well 1= DNA marker 1kb (1000bp), Well 2-15= Sample.

Figure 2:

Electrophoresis results for PCR products 2% agarose concentration. Note: well 1= DNA marker 100bp, Well 2-11= Sample.

Figure 3:

Visualization of RFLP results with 2% electrophoresis. Note: Sample number 1-13, 13 samples produce two bands at 66 bp and 40 bp.

Equation 1

Advances in Animal and Veterinary Sciences

December

Vol. 12, Iss. 12, pp. 2301-2563

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