Ganoderma lucidum as a Biocontrol agent for Management of Alternaria solani, A Pathogen of Early Blight of Tomato
Muhammad Asif1*, Ahmad Ali Shahid1,2 and Nasir Ahmad3
1Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; 2Center of Excellence and Molecular Biology, University of the Punjab, Lahore, Pakistan; 3School of Biological Sciences, University of the Punjab Lahore, Pakistan.
*Correspondence | Muhammad Asif, Insititute of Agricultural Sciences, University of the Punjab, Lahore, Pakistan; Email: ali_asif514@yahoo.com
Figure 1:
A) Purigied mycelial growth, B) Microphotograph of conidial strutures of Alternatia solani on PDA at 100x (Labomed CXL, Labo America inc.)
Figure 2:
A) Basidiocatp, B) Mycelial growth, C) Hmenium, D) Basidiospores of G. lucidum.
Figure 3:
Antagonistic effect of G. lucidum against A. solani; A) Control (A. solani) B anc C) Dual culture (G. lucidum in white colony and A. solani in black colony showing growth zone of inhibition).
Figure 4:
Activity assay of crude protein extract aginst A. solani. Different alphabet letters indicate significant difference at 0.05 probability level.
Figure 5:
Antigungal potential of crude protein extract of G. lucidum agaist A. sonani. A) Control, B) 0.5mg/ml, C)1mg/ml, D) 2mg/ml.
Figure 6:
MIC of crude protein against A. solani by using Microspectropotmetry. Different alphabet letters indicate significant difference at 0.05 probability level.