Identification of ADPKD-Related Key miRNAs by Constructing a miRNA-mRNA Interaction Network and Experimental Verification
Identification of ADPKD-Related Key miRNAs by Constructing a miRNA-mRNA Interaction Network and Experimental Verification
Hui Fang*, Kai Xue and Teng Yang
Fig. 1.
DEMs and DEGs screening. a, DEMs screening (2 DEMs were screened, 1 up and 1 down). b, DEGs screening (1090 DEGs were screened).
Fig. 2.
miRNA-mRNA network expression in ADPKD.
Fig. 4.
The expression levels of target genes (A and B) and (C-F) the alteration of target gene after transfection in WT9-12.
a, miR-199a-3p miRNA; b, miR-107 miRNA; c, PRKCE; d, TMED5; e, RUNX1T1; f, FGF2.
Notes: n= 6 per group. # P <0.05 vs. NC. Data are Mean ± SE. Similar results were obtained after repeating the experiment three times.
Fig. 3.
qRT-PCR validation of target mRNA expression in HK-2 and WT9-12 cells. a, PRKCE; b, RUNX1T1; c, SMARCE1; d, CALU; e, FGF2; f, TMED5; g, PNRC1.
Notes: n = 6 per group; *P <0.05 vs. HK-2. Data are Mean ± SE, results were obtained after repeating the experiment three times.
December 2023
Pakistan J. Zool., Vol. 55, Iss. 6, pp. 2501-3000
