Research Article

Impact of Ova Injection with Zinc Methionine on Some Blood Parameters and Glycogen Level of Broiler Chickens’ Exposed to Feed Fasting

Salman K.A.A1, Mohammed Khalil Ibrahim Al-Saeedi2, Hashim Hadi Al-Jebory3*

1College of Agricultural Engineering Sciences, Baghdad University, Iraq; 2College of Environmental Sciences, Al-Qasim Green University, Iraq; 3Agriculture College, Al-Qasim Green University, Iraq.

Received | April 13, 2024; Accepted | May 22, 2024; Published | June 27, 2024

*Correspondence | Hashim Hadi Al-Jebory, Agriculture College, Al-Qasim Green University, Iraq; Email: hashimhadi@agre.uoqasim.edu.iq

Citation | Salman KAA, Al-Saeedi MKI, Al-Jebory HH (2024). Impact of ova injection with zinc methionine on some blood parameters and glycogen level of broiler chickens exposed to feed fasting. Adv. Anim. Vet. Sci., 12(8):1532-1538.

DOI | https://dx.doi.org/10.17582/journal.aavs/2024/12.8.1532.1538

ISSN (Online) | 2307-8316

Copyright: 2024 by the authors. Licensee ResearchersLinks Ltd, England, UK.

This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).

Introduction

The fact that the chicks remain in the hatchery until their completion is a significant problem that must be addressed, as the chicks are exposed to tremendous pressure throughout the sorting and counting process, which lasts for a day or two without food or water, which leads to weight loss and weak immunity, and in order to improve growth after hatching, With live body weight, weight gain, and reduced embryo mortality, early feeding which includes injecting hatching eggs with a nutrient solution is believed to be critical (Dalouha et al., 2008). In Edens (2000), external feeding has improved the young’s hatchability, gastrointestinal development, body weight, and nutritional status (Uni and Verkt, 2004). Vitamins and trace elements, which are antioxidant components, are essential for protecting embryo tissues throughout their growth and development (Eid et al., 2003). In addition, because of the embryo’s increased metabolic rate, the embryo’s stores of nutrients are insufficient during the Pre-hatching stage (Yair et al., 2013). As a result, methods of introducing nutrients and nutritional supplements before hatching have been explored (Oliveira et al., 2015); one of these methods, the egg injection method, effectively intro-duces additional nutrients required for embryonic development (Foye et al., 2007; Kadam et al., 2008; Dlouha, 2008), as well according to Prasad and Kucuk (2002), Shaheen et al. (2009), and Salem et al. (2011), zinc is an essential trace element involved in more than 300 enzymes involved in carbohydrate and energy metabolism, protein synthesis and degradation, DNA synthesis, carbon dioxide transport, and many other reactions, zinc can be used as organic zinc (com-plexes comprising proteins and amino acids), inorganic zinc, or as a component of carbohydrates. Due to the higher bioavailability of organic zinc, organic sources of zinc have been gradually introduced in ova injection or broiler diets (Salim et al., 2011). Zinc methionine (ZnM) is organic zinc that is not chelated in the intestinal lumen by phytic acid because it contains no free divalent cations (Zaki and Al-Jebory, 2021; Al-Saeedi et al., 2022). Thus, it undergoes distinct metabolic pathways, improving zinc uptake (Burrell et al., 2004). Thus, this study aimed to ascertain how the injection of zinc and thief of hatching eggs affects glycogen levels and some blood markers in broiler chicks subjected to dietary fasting.

Materials and Methods

From February 5, 2020, to February 25, 2020, this experiment was carried out in the hatchery of Al-Shaflawi Poultry Company in the Al-Mahaweli Governorate, Babylon. Using a computerized electronic scale type SF-400, I weighed 300 eggs and found their average weight was 53±1 grams. They were injected with a zinc methionine solution in varying amounts on the eighteenth day of incubation. Following hatching, 15 chicks from each treatment were removed and fasted for 48 hours. One hour, Four treatments were used for the hatching egg treatments. They were as follows: T1: Injection 0.3 ml/ egg NaCl solution as a control treatment, T2, T3, and T4 injection 0.3 ml/ egg from zinc-methionine solution with levels (60, 80, 100 ppm). These concentrations were used by previous studies and in a manner consistent with the toxicity of zinc and methionine to embryos.

Solutions for injecting eggs

The egg injection solution was made using distilled water, and zinc-methionine powder was purchased from Zinpro, a US business.

Studied traits

Physiological traits

Following hatching, blood samples were taken from chicks exposed to different food designs (6, 12, 24, and 48), and certain physiological traits were assessed. The levels of glucose, cholesterol, and the enzymes ALT and AST were measured in the chicks’ blood serum. Franey and Elias (1968) disclosed a method for assessing cholesterol levels using measurement equipment (Kit) from the German company Roche. The German business Roche provided the measuring instrument (Kit), which was used to assess the glucose levels. The concentration of triglycerides was estimated using the equation given by Grudy et al. (2004) in the method (Reitman and Frankel, 1957), and the enzymes ALT and AST were estimated using a measuring device (Kit) from the German company Roche. The anthrone technique calculates glycogen in the muscles, liver, and heart (Havenstein et al., 2007).

Statistical analysis

Multiple range tests were employed to analyze the significant differences between means, and a fully randomized design (CRD) was utilized to examine the impact of various treatments on all attributes (Duncan, 1955). The statistical analysis system (SAS, 2012) was used.

Results and Discussion

Table 1 displays the results of injecting zinc methionine into hatching eggs. T3 and T4 had lower cholesterol concentrations at six o’clock than T1 and T2; at 12, 24, and 48 hours, the T2, T3, and T4 treatments decreased significantly more than the T1 treatment.

Table 2 shows the effect of the study on triglyceride; at six hours, the T2 treatments had heights significantly (P≤ 0.05) compared to other treatments, and T1 increased in the T3 treatment, as well at 12 hours, the T1 and T2 treatments came heights (P≤ 0.05) respectively compared to T3 and, T4. Meanwhile, in 24 hours, there was no significant difference between treatments; in 48 hours, the T1 had a significant (P ≤ 0.05) increase compared to T3 and T4 treatments, and at the same time, the T3 treatment increased to the T4 treatment.

Table 3 shows the effect of treatments on glucose level at six hours of the T1 treatment had the heights (P≤ 0.05) on T3 and T4 while T3 increased on T4 treatment, in 12 hours to the T1 increase significantly (P≤ 0.05) compared other treatments at the same time the T3 and T4 treatments increase on T2 treatment.

 

Table 1: Impact of Ova Injection with zinc methionine on the cholesterol concentration of chicks exposed to fasting. (mean ± standard error).

Mean±stander error				Treatments
Cholesterol
48 h	24 h	١٢ h	Six h	Time
92.92±2.95 a	100.28±0.71 a	136.33±0.66 a	129.80±1.74 a	T1
82.06±1.47 b	67.91±2.87 c	123.47±2.10 b	128.72±1.85 a	T2
63.37±2.50 c	87.50±0.30 b	118.59±2.75 b	134.09±1.10 b	T3
79.21±1.76 b	84.12±1.00 b	105.62±1.84 c	107.90±1.16 c	T4
*	*	*	*	Significant

 

A significant difference was shown with different letters at 0.05*; T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table ٢: Impact of Ova Injection with zinc methionine on the triglyceride concentration of chicks exposed to fasting (mean ± standard error).

Mean±stander error				Treatments
Triglyceride
48 h	24 h	١٢ h	Six h	Time
70.83±1.87 a	57.42±2.28	97.13±2.01 a	70.67±1.25 b	T1
60.76±2.06 bc	55.29±1.48	83.60±0.94 b	75.55±2.18 a	T2
65.77±1.61 b	56.02±0.20	72.10±1.98 c	60.26±0.29 c	T3
55.40±0.45 c	57.58±1.99	73.92±1.75 c	66.65±1.50 bc	T4
*	NS	*	*	Significant

 

A significant difference was shown by means with different letters at the level 0.05*, NS not significant. T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table 3: Impact of Ova injection with zinc methionine on the glucose concentration of chicks exposed to fasting (mean ± standard error).

Mean±stander error				Treatments
Glucose
48 h	24 h	١٢ h	Six h	Time
162.60±2.95	179.38±2.93	198.83±2.05 a	215.48±3.20 a	T1
160.53±1.01	178.11±1.89	151.24±1.99 c	208.62±1.24 ab	T2
166.62±1.37	179.48±2.79	182.33±1.44 b	198.63±1.36 b	T3
160.05±2.17	172.49±3.16	181.57±1.68 b	172.29±1.21 c	T4
NS	NS	*	*	Significant

 

A significant difference was shown by means with different letters at the level 0.05*, NS not significant. T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

The zinc methionine treatments had the most excellent value GSH-px compared to the control treatment, and the T3 and T4 treatments were the best in 24 and 48 hours, respectively. Meanwhile, there was no significant difference among treatments in MDA.

Table 5 shows the effect of the study on the liver enzymes; there was no significant difference at 24 hours in AST enzyme and all time for ALT enzyme. As well as at 48 hours in AST enzyme, the T1 and T2 treatments were the heights (P≤ 0.05), respectively on T3 and T4 treatments.

Table 6 shows the effect of the treatments on liver glycogen; at six and 24 hours, there was a significant increase (P≤ 0.05) for T4 treatment on other treatments at the same time significant increase for the T2 and T3 treatments compared T1, at 12 hours the T4, and T3 were the heights significantly (P≤ 0.05) on T1, and T2 respectively, while at 48 hours the T3, and T4 had increase (P≤ 0.01) compared T1, and T2 treatments, the T1 increase on T2 treatment.

Table 7 shows that at hours 6 and 12, heart glycogen in T3 and T4 was significantly higher than in T1 and T2. Similarly, at hour 24, T3 and T4 had higher heart glycogen values than T1 and T2, with the T4 treatment having the most significant effect. T3 and T4 performed better than T1 and T2 after 48 hours, with T3 having the highest value for this trait.

 

Table ٤: Impact of Ova Injection with zinc methionine on the concentration of some triglyceride antioxidants in chicks exposed to fasting (mean ± standard error).

Mean±stander error				Treatments
MDA		GSH-px
48 h	24 h	48 h	24 h	Time
2.27±0.14	3.42±0.22	42.27±0.89 c	50.76±1.01 c	T1
2.36±0.31	2.98±0.75	50.21±0.50 ab	57.14±0.58 b	T2
2.31±0.24	3.15±0.18	48.37±0.28 b	63.09±0.55 a	T3
2.11±0.15	3.35±0.89	53.84±1.00 a	59.18±0.25 b	T4
NS	NS	*	*	Significant

 

A significant difference was shown by means with different letters at the level 0.05*, NS not significant. T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table ٥: Impact of Ova Injection with zinc methionine on the concentration of some liver enzymes in chicks exposed to fasting. (mean ± standard error).

Mean±stander error				Treatments
ALT		AST
48 h	24 h	48 h	24 h	Time
2.28±0.01	4.51±0.09	65.19±0.50 a	44.51±0.13	T1
2.21±0.04	4.33±0.10	56.36±0.24 b	45.17±0.51	T2
2.19±0.05	3.95±0.02	51.63±1.00 c	43.22±0.81	T3
2.30±0.08	4.45±0.06	53.71±0.62 c	44.36±0.95	T4
NS	NS	*	NS	Significant

 

A significant difference was shown by means with different letters at the level 0.05*, NS not significant. T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table 6: Impact of Ova Injection with zinc methionine on the liver glycogen concentration of chicks exposed to fasting. (mean ± standard error).

Mean±stander error				Treatments
Liver glycogen
48 h	24 h	١٢ h	Six h	Time
10.19±0.55 b	8.94±0.02 d	10.73±0.12 c	10.37±0.97 c	T1
8.98±0.20 c	11.91±0.39 c	11.00±1.52 c	14.55±1.30 b	T2
15.94±0.24 a	14.27±0.72 b	15.66±0.66 b	16.45±0.54 b	T3
16.87±0.83 a	19.17±0.48 a	19.28±0.29 a	20.67±0.58 a	T4
**	**	*	*	Significant

 

A significant difference was shown using different letters at 0.05* and 0.01**. T1 was the control treatment that injected NaCl, while T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table ٧: Impact of in Ova Injection with zinc methionine on Heart glycogen concentration of chicks exposed to fasting. (mean ± standard error).

Mean±stander error				Treatments
Heart glycogen
48 h	24 h	١٢ h	Six h	Time
12.38±1.01 c	10.72±1.49 c	10.67±1.70 b	12.49±0.93 b	T1
12.78±0.17 c	10.94±0.29 c	11.66±0.88 b	13.24±1.16 b	T2
16.87±0.92 a	16.33±0.51 b	17.65±0.67 a	18.35±0.39 a	T3
14.90±0.44 b	19.23±1.58 a	17.69±1.46 a	18.00±0.52 a	T4
*	*	*	*	Significant

 

A significant difference was shown using different letters at the level 0.05*. T1 was the control treatment that injected NaCl, T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table ٨: Impact of in Ova Injection with zinc methionine on the concentration of triceps muscle glycogen in chicks exposed to fasting. (mean ± standard error).

Mean±stander error				Treatments
Muscles glycogen
48 h	24 h	١٢ h	Six h	Time
15.28±1.07 c	19.80±1.23 b	17.24±1.30	16.91±1.54	T1
15.10±0.35 c	18.16±1.15 b	15.92±2.06	17.57±1.18	T2
19.31±1.98 a	21.95±2.04 a	16.92±2.92	18.27±2.75	T3
17.73±0.36 b	20.64±1.36 a	16.25±1.80	17.83±1.83	T4
**	*	NS	NS	Significant

 

A significant difference was shown by means with different letters at 0.05*,0.01**, and NS, which were insignificant. T1 was the control treatment that injected NaCl, while T2, T3, and T4 injected 0.3 ml of zinc-methionine solution per egg, with 60, 80, and 100 ppm, respectively.

 

Table 8 shows that at hours 6 and 12, there were no significant differences in muscle glycogen concentrations between the treatments; the quantity of muscle glycogen was increased at T3 and T4 than at T1 and T2, although there were significant differences (P ≤ 0.05) at hour 24. There was a very significant increase (P≤ 0.01) at hour 48, as treatment T3 recorded the highest significance for this treatment; it was more significant in T3 and T4 compared to T1 and T2.

Injections and treatments containing methionine zinc have proven effective in controlling glucose, glycogen, triglycerides, and cholesterol levels; zinc is one of the essential microminerals needed for development and is involved in several metabolic pathways, which explains why (Ezzati et al., 2013). Because of its role in building physiological tissues, boosting the production of muscles, proteins, and enzymes, improving avian health, and increasing the quantity of This is because zinc is necessary for the skeleton’s growth and for critical regulatory mechanisms that result in the synthesis of collagen, bone, and cartilage (Oliveira et al., 2015). By lessening the harm that oxidative stress produces, zinc also enhances the health of broiler chicks (Salami et al., 2015). This is because zinc stimulates the synthesis of metallothionine, a material abundant in p-glycoprotein. Zn binds and serves as a cofactor for superoxide dismutase and other antioxidants. According to Valko et al. (2016), it is connected to enzymes that preserve the integrity of cells connected to the immunological response, improving broiler chickens’ physiological performance and productivity. Zinc can also be absorbed in the stomach and attach to the intestinal protein metallothionein; alternatively, albumin can carry it to the liver, according to Prasad (1993) and Oteiza (1996). By attaching to cell membranes, Zinc functions as a direct antioxidant in biological tissues by reducing the generation of free radicals and replacing certain metallic elements like iron (Fe) and copper (Cu) at their union sites (Powell, 2000; Prasad and Kucuk, 2002). In ova, injecting zn-me into the hatching eggs may have some impact because methionine is engaged in various metabolic pathways within the body (Chen et al., 1993). Zinc aids the body in avoiding oxidative stress by preventing lipid peroxidation and glutathione depletion (Prasad, 1997).

Zinc reduces oxidative stress and promotes antioxidants, so AST enzyme activity at T2, T3, and T4 is reduced in birds (Richard and Preston, 2006). Zinc may protect polyunsaturated fatty acids from oxidative damage by acting as an antioxidant in cell and liver membranes and maintaining the liver’s selective permeability. This membrane prevents enzyme leakage from the cell’s interior to the outside, it accomplishes this by scavenging free radicals, interrupting the cascade of free radical reactions, and preventing metal ions from exiting the tissues. Alternatively, methionine might be the reason since it promotes oxidation and helps produce the enzyme glutathione peroxidase. Zinc methionine injection has a high concentration of glutathione peroxidase enzyme since methionine is required to synthesize this enzyme. These prior findings align with the results of Shin et al. (2015) and our study.

Conclusions and Recommendations

Based on previous results that showed an improvement in the levels of cholesterol, triglycerides, glucose, AST, and glycogen when zinc methionine was injected, this is because zinc has antioxidant properties, helps build body tissues, and stimulates the building of protein, muscles, glycogen, and enzymes. Zinc may have a positive role in enhancing the growth and digestion of nutrients, which is reflected positively in the performance and immunity of chicks; various types of stress, including food, also lead to a deterioration in the glycogen level, especially in the muscles, this is due to the hormone corticosterone, which causes the catabolism of glycogen, the results showed an improvement in the level of glycogen in the treatments compared to the control, this gives an idea of the importance of methionine and zinc in the processes of construction and catabolism within the cells, and that they are essential, in many metabolic pathways and the manufacture of antioxidant compounds, it could be a future study using nano-methionine-zinc, as they may have a more significant role in improving the performance of hatched chicks.

Acknowledgement

The authors would like to express their special thanks and gratitude to the Department of Animal Production/College of Agriculture/ Al-Qasim Green University, Baghdad University, and Al-Anwar company for all their kind and helpful support during the study period.

Novelty Statement

This study is the first in Iraqi/Babylonian government to use zinc-methionine to enhance chick growth.

Author’s Contribution

Each contributor made an equal contribution to the paper.

Conflict of interest

The authors have declared no conflict of interest.

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