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Improving the Efficiency of Campylobacter spp. Isolation from Livestock and Poultry in Ukraine

Improving the Efficiency of Campylobacter spp. Isolation from Livestock and Poultry in Ukraine

Natalia Shchur1,2*, Tetiana Mazur1, Orest Katsaraba3, Ihor Halka2, Ludmila Shalimova2, Larisa Moskalenko4, Tetiana Ponomariova-Herasymiuk4, Maksym Lusta4, Vitalii Nedosekov1

1Department of Epizootology, Microbiology and Virology, Faculty of Veterinary Medicine The National University of Life and Environmental Sciences of Ukraine, Potehin Str., 16, Kyiv, Ukraine; 2State Scientific Research Institute for Laboratory Diagnostics and Veterinary and Sanitary Expertise, Donetska Str., 30, Kyiv, Ukraine; 3Stepan Gzhytskyi National University of Veterinary Medicine and Biotechnologies Lviv, Pekarska Str., 50, Lviv, Ukraine; 4Communal non-commercial enterprise, Dniprovsk City Clinical Hospital No. 9, of the Dnipro City Council, Manuilivskyi Аve., 29, Dnipro, Ukraine.

 
*Correspondence | Natalia Shchur, Department of Epizootology, Microbiology and Virology, Faculty of Veterinary Medicine The National University of Life and Environmental Sciences of Ukraine, Potehin Str., 16, Kyiv, Ukraine; Email: [email protected]

Figure 1:

Campylobacter spp. in mixed culture. Gram-negative, thin, spirally curved bacteria that have one complete curl or resemble a ‘comma’ or ‘gull wings’. Gram stain. View under a Leica DM 5000 light microscope (immersion objective 100).

Table 1:

The results of isolation of Campylobacter spp. by the direct application method. 

Table 2:

The results of isolation of Campylobacter spp. according to the enrichment method.

Figure 2:

Cream colonies of Campylobacter spp. from a round shape with a shiny convex surface to the formation of a plaque on the surface of the medium with the effect of “swarming”. Campylobacter Agar, 24-hour cultivation.

Figure 3:

Greyish colonies of Campylobacter spp. on mCCD agar from rounded shape with metallic sheen to formation of plaque on the surface of the medium with a “swarming” effect, 24-hour cultivation.

Figure 4:

The cocci-like form of Campylobacter spp. Fuchsin staining. View under a Leica DM 5000 light microscope (immersion objective 100).

Advances in Animal and Veterinary Sciences

February

Adv. Anim. Vet. Sci., Vol. 13, Iss. 2,

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