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Isolation and Genetic Characterization of Hobi- Like Pestivirus Circulating in Egyptian Cattle during 2019-2020

Isolation and Genetic Characterization of Hobi- Like Pestivirus Circulating in Egyptian Cattle during 2019-2020

Zeinab R. Aboezz1*, Ayman S. El-Habbaa1, Rania S. El-Mohamady2, Samia A. Elnagar2, Ehab M. El-Nahas1

1Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt; 2Viral Diseases Research Unit, Animal Reproduction Research Institute (ARRI), Agricultural Research Center (ARC), Giza, Egypt.


*Correspondence | Zeinab Aboezz, Virology Department, Faculty of Veterinary Medicine, Benha University, Moshtohor 13736, Kalubia, Egypt; Email: [email protected]

Figure 1:

Neighbor-joining Phylogenetic tree for partial 5` UTR nucleotide sequence of BVDV- Egypt-020-1ncp (MZ873104) and BVDV-Egypt-020-3ncp (MZ873105). strains compared with other BVDV-1, BVDV-2 and BVDV-3 reference sequence obtained from GenBank database. The scale bar represents the number of substitutions per nucleotide. Egypt-020-1ncp and BVDV-Egypt-020-3ncp strains from the present study are indicated by black circle.

Figure 2:

Nucleotide variations in 288bp 5UTR products of BVDV-Egypt-020-1ncp (original serum sample) and BVDV-Egypt-020- 2ncp (third passage on MDBK cells from serum). Dots or hide refer to nucleotide similarities while box refer to nucleotide differences.

Figure 3:

Nucleotide variations in 288bp 5UTR products of BVDV-Egypt-020-1ncp (original serum sample) and BVDV-Egypt-020- 3ncp (original ovary sample). Dots or hide refer to nucleotide similarities while box refer to nucleotide differences.

Advances in Animal and Veterinary Sciences

February

Adv. Anim. Vet. Sci., Vol. 13, Iss. 2,

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